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A transgenic model for isolation of ...

Duke University., Pathology.

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A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging./
作者:	Jayawardena, Tilanthi Malaka.
面頁冊數:	148 p.
附註:	Advisers: Daniel J. Kenan; Salvatore V. Pizzo.
Contained By:	Dissertation Abstracts International69-12B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3337164
ISBN:	9780549918035

A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging.
Jayawardena, Tilanthi Malaka.

A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging. - 148 p.

Advisers: Daniel J. Kenan; Salvatore V. Pizzo.

Thesis (Ph.D.)--Duke University, 2008.

Angiogenesis, the growth of new blood vessels, is required for the progression of proliferative diseases such as cancer. The identification of angiogenesis as a "common denominator" in society's most important diseases has lead to a widespread effort to define the molecular mechanisms that regulate the growth, remodeling, and maintenance of new blood vessels. Endothelial cells (EC) line the inner surface of blood vessels and play a significant role in regulating the balance between states of vascular quiescence and active remodeling. Previous reports suggest that ECs from discrete vascular beds have distinct gene expression profiles and subsequently specialized functional roles. Characterizing the differential expression patterns exhibited by the tumor vasculature will guide the development of therapeutic and diagnostic agents that directly target cancer.

ISBN: 9780549918035Subjects--Topical Terms:

1017719
Biology, Molecular.

A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging.
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100 1 $a Jayawardena, Tilanthi Malaka. $3 1064510
245 1 2 $a A transgenic model for isolation of endothelial-specific transcripts by ribonucleoprotein tagging.
300 $a 148 p.
500 $a Advisers: Daniel J. Kenan; Salvatore V. Pizzo.
500 $a Source: Dissertation Abstracts International, Volume: 69-12, Section: B, page: 7293.
502 $a Thesis (Ph.D.)--Duke University, 2008.
520 $a Angiogenesis, the growth of new blood vessels, is required for the progression of proliferative diseases such as cancer. The identification of angiogenesis as a "common denominator" in society's most important diseases has lead to a widespread effort to define the molecular mechanisms that regulate the growth, remodeling, and maintenance of new blood vessels. Endothelial cells (EC) line the inner surface of blood vessels and play a significant role in regulating the balance between states of vascular quiescence and active remodeling. Previous reports suggest that ECs from discrete vascular beds have distinct gene expression profiles and subsequently specialized functional roles. Characterizing the differential expression patterns exhibited by the tumor vasculature will guide the development of therapeutic and diagnostic agents that directly target cancer.
520 $a We have developed a novel ribonucleoprotein (RNP) technology that enables the isolation of tumor endothelium-specific mRNAs from bulk tumor and host tissues. The RNP-tag is an engineered RNA-binding protein, Poly (A) binding protein (PABP) that binds almost all actively translated mRNAs within a cell. The PABP is tagged with an engineered epitope to enable specific co-immunoprecipitation of all tagged PABP complexes within a tissue sample. Specific expression of the RNP-tag using the Tie2 endothelium-specific promoter in transgenic mice ensures that RNP-tag is expressed only within the vascular endothelium of any transgenic tissue. Addition of tag-specific antibodies to a total tissue extract from a tumor formed in a transgenic mouse therefore enables the robust enrichment of tumor endothelium mRNAs. The enriched mRNAs can be read out using a number of sensitive analytic methods, including RNAse protection assay, quantitative real-time PCR, microarrays, and deep sequencing approaches.
520 $a We present here the construction and validation of a transgenic mouse expressing Flag-tagged PABP under the control of the Tie2 promoter. Our data show that, in our transgenic mouse model, Flag-tagged PABP is expressed specifically in the vascular endothelium. Additionally, quantitative real-time PCR and microarray analyses indicate that the RNP-tag platform represents a specific and efficient tool that can be used to discover changes in vascular endothelial gene expression. We anticipate use of the technology to identify novel vascular addresses that could significantly improve cancer diagnostics, therapeutics and imaging.
590 $a School code: 0066.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Medicine and Surgery. $3 1017756
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710 2 0 $a Duke University. $b Pathology. $3 1064509
773 0 $t Dissertation Abstracts International $g 69-12B.
790 $a 0066
790 1 0 $a Abraham, Soman N. $e committee member
790 1 0 $a Dewhirst, Mark W. $e committee member
790 1 0 $a Hale, Laura P. $e committee member
790 1 0 $a Keene, Jack D. $e committee member
790 1 0 $a Kenan, Daniel J., $e advisor
790 1 0 $a Kontos, Christopher D. $e committee member
790 1 0 $a Pizzo, Salvatore V., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3337164