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Electrical detection of DNA and inte...

Princeton University.

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Electrical detection of DNA and integration with nano-fluidic channels.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Electrical detection of DNA and integration with nano-fluidic channels./
Author:	Tung, Chih-Kuan.
Description:	135 p.
Notes:	Adviser: Robert H. Austin.
Contained By:	Dissertation Abstracts International69-10B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3332433
ISBN:	9780549847663

Electrical detection of DNA and integration with nano-fluidic channels.
Tung, Chih-Kuan.

Electrical detection of DNA and integration with nano-fluidic channels. - 135 p.

Adviser: Robert H. Austin.

Thesis (Ph.D.)--Princeton University, 2008.

The elongation of genomic length DNA in confining nanochannels is not only a fascinating exercise in polymer dynamics, but also is of great interest in biotechnology because the elongation of the confined molecule is directly proportional to the actual length of the molecule in basepairs. Precision length measurements of genomic length DNA molecules are useful because most of the mutations are not point mutations, but a rearrangement, insertion or deletion of a variety of lengths of segments of the genome within the genome itself. Conducting such measurements without an expensive optical microscope will be of great value for genomic analysis, and electrical measurements should provide the highest resolution. In this thesis, measurement methods using field-effect transistors (FETs) and electrochemical measurements are presented, and we demonstrate methods to integrate the electronic devices with the nano-fluidics.

ISBN: 9780549847663Subjects--Topical Terms:

1019105
Biophysics, General.

Electrical detection of DNA and integration with nano-fluidic channels.
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100 1 $a Tung, Chih-Kuan. $3 1036440
245 1 0 $a Electrical detection of DNA and integration with nano-fluidic channels.
300 $a 135 p.
500 $a Adviser: Robert H. Austin.
500 $a Source: Dissertation Abstracts International, Volume: 69-10, Section: B, page: .
502 $a Thesis (Ph.D.)--Princeton University, 2008.
520 $a The elongation of genomic length DNA in confining nanochannels is not only a fascinating exercise in polymer dynamics, but also is of great interest in biotechnology because the elongation of the confined molecule is directly proportional to the actual length of the molecule in basepairs. Precision length measurements of genomic length DNA molecules are useful because most of the mutations are not point mutations, but a rearrangement, insertion or deletion of a variety of lengths of segments of the genome within the genome itself. Conducting such measurements without an expensive optical microscope will be of great value for genomic analysis, and electrical measurements should provide the highest resolution. In this thesis, measurement methods using field-effect transistors (FETs) and electrochemical measurements are presented, and we demonstrate methods to integrate the electronic devices with the nano-fluidics.
520 $a Since DNA molecules are negatively charged in an aqueous solution, FETs can be used to detect the electric fields from the molecules. Our studies show that different characteristic I-V curves are seen when DNA molecules are present in the solution, for FET devices made of carbon nanotubes and poly-silicon film. We also demonstrate that some easy electrical measurements can be used to detect DNA or the bases of DNA by their electrochemical properties.
520 $a To integrate the electrical detection with the fluidics, we present two different methods to achieve that goal. A channel made with silicon oxide lift-off and anodic bonding can be used to integrate electronics with microchannels, and a self-sealed parylene capped nanochannel can be easily integrated with the electronics. The ability to integrate electronics with nanochannels can eventually lead to an all-integrated single-piece genomic diagnostic system.
590 $a School code: 0181.
650 4 $a Biophysics, General. $3 1019105
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710 2 $a Princeton University. $3 645579
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790 1 0 $a Austin, Robert H., $e advisor
791 $a Ph.D.
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