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Sequence diversity and expression of...

University of Puerto Rico, Mayaguez (Puerto Rico).

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Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments./
作者:	Rodriguez-Castano, Gina Paola.
面頁冊數:	182 p.
附註:	Adviser: Arturo A. Massol-Deya.
Contained By:	Masters Abstracts International47-02.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1458836
ISBN:	9780549798422

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
Rodriguez-Castano, Gina Paola.

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments. - 182 p.

Adviser: Arturo A. Massol-Deya.

Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.

Nitrous oxide (N2O) is a greenhouse gas produced in different microbial processes. Nitrous oxide reductases (N2ORs) are enzymes that catalyze N2O transformation to N2. Understanding the diversity of nitrous oxide reductases genes (nosZ ) is crucial to predict and manage emissions. This study developed oligonucleotides for amplifying a broader range of nosZ genes to assess their diversity in different environments. Sequences obtained were different from traditional nosZ sequences (62% amino acid similarity). Neither of the clone sequences fell into the traditional NosZ phylogenetic clades, but grouped with nontraditional NosZ sequences from Anaeromyxobacter spp., Magnetospirillum spp., Desulfitobacterium hafniense, and Dechloromonas aromatica. The detection of nosZ genes in the environment was achieved by ISRT-PCR/FISH using an internal fluorescently-labeled NosZ943 probe. We demonstrate the existence of many variants of nosZ gene. These variants could represent a high functional diversity for reducing N2O.

ISBN: 9780549798422Subjects--Topical Terms:

1017734
Biology, Microbiology.

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
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245 1 0 $a Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
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502 $a Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.
520 $a Nitrous oxide (N2O) is a greenhouse gas produced in different microbial processes. Nitrous oxide reductases (N2ORs) are enzymes that catalyze N2O transformation to N2. Understanding the diversity of nitrous oxide reductases genes (nosZ ) is crucial to predict and manage emissions. This study developed oligonucleotides for amplifying a broader range of nosZ genes to assess their diversity in different environments. Sequences obtained were different from traditional nosZ sequences (62% amino acid similarity). Neither of the clone sequences fell into the traditional NosZ phylogenetic clades, but grouped with nontraditional NosZ sequences from Anaeromyxobacter spp., Magnetospirillum spp., Desulfitobacterium hafniense, and Dechloromonas aromatica. The detection of nosZ genes in the environment was achieved by ISRT-PCR/FISH using an internal fluorescently-labeled NosZ943 probe. We demonstrate the existence of many variants of nosZ gene. These variants could represent a high functional diversity for reducing N2O.
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