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Mitochondrial DNA analyses for speci...

University of Puerto Rico, Mayaguez (Puerto Rico).

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Mitochondrial DNA analyses for species identification of snappers from Caribbean waters.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Mitochondrial DNA analyses for species identification of snappers from Caribbean waters./
Author:	Rodriguez-Santiago, Aurea E.
Description:	81 p.
Notes:	Adviser: Ernesto Otero Morales.
Contained By:	Dissertation Abstracts International69-09B.
Subject:	Agriculture, Fisheries and Aquaculture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3327352
ISBN:	9780549796909

Mitochondrial DNA analyses for species identification of snappers from Caribbean waters.
Rodriguez-Santiago, Aurea E.

Mitochondrial DNA analyses for species identification of snappers from Caribbean waters. - 81 p.

Adviser: Ernesto Otero Morales.

Thesis (Ph.D.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.

This study examined phylogenetic relationships among fifteen species of the Lutjanidae family occurring within the Caribbean Basin, based on mitochondrial 12S rDNA analysis. Previous investigations have limited their scope to species occurring in the western Atlantic (WA) and Cuba, or to several species within the Lutjaninae subfamily. This is the first phylogenetic study that includes all 3 subfamilies of lutjanids occurring in the Caribbean. We identified diagnostic polymorphisms within the mitochondrial DNA (mtDNA) for 15 lutjanid species in the Caribbean. Specimens were obtained from local catches at La Parguera, Puerto Real and Rincon, western Puerto Rico and Catano, northern Puerto Rico. DNA variation was quantified through the use of polymerase-chain-reaction (PCR) amplification of fragments corresponding to 450 bp of the mtDNA 12S rRNA gene followed by sequencing. Intraspecific variation was not found within any species for the adult specimens analyzed during this study. Assessment of phylogenetic relationships of species was conducted using Neighbor Joining (NJ) and Bayesian Inference (BI) analyses. Phylogenetic relationships within the subfamily Lutjaninae remained rather unresolved for some species. Nevertheless, our study suggests that even if not strongly supported, the groups found are associated according to morphology, habitat or feeding preferences. In addition, the consistency in the sequence data for each species in this study demonstrates that the 12S rRNA gene is a reliable tool for taxonomic identification within this family. As these sequences constitute a sort of molecular key for all the 15 species of lutjanids studied, those were used to identify unknown samples of lutjanid larvae. Larval specimens were obtained from plankton tows using a 300 mum mesh net. Seven species were identified among the collected larvae: Lutjanus apodus, Lutjanus synagris, Lutjanus analis, Lutjanus griseus, Lutjanus mahogoni, Ocyurus chrysurus and Rhomboplites aurorubens. Identification of these larvae increases our understanding of early larval stages taxonomy. In addition, this information is useful for the design of research leading to the description of spawning, dispersal and recruitment patterns, as well as habitat selection for the species. These analyses are of vital relevance for assessments regarding the establishment of Marine Protected Areas (MPAs) as a management option to restore diminishing stocks of fish populations.

ISBN: 9780549796909Subjects--Topical Terms:

1020913
Agriculture, Fisheries and Aquaculture.

Mitochondrial DNA analyses for species identification of snappers from Caribbean waters.
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245 1 0 $a Mitochondrial DNA analyses for species identification of snappers from Caribbean waters.
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500 $a Adviser: Ernesto Otero Morales.
500 $a Source: Dissertation Abstracts International, Volume: 69-09, Section: B, page: 5246.
502 $a Thesis (Ph.D.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.
520 $a This study examined phylogenetic relationships among fifteen species of the Lutjanidae family occurring within the Caribbean Basin, based on mitochondrial 12S rDNA analysis. Previous investigations have limited their scope to species occurring in the western Atlantic (WA) and Cuba, or to several species within the Lutjaninae subfamily. This is the first phylogenetic study that includes all 3 subfamilies of lutjanids occurring in the Caribbean. We identified diagnostic polymorphisms within the mitochondrial DNA (mtDNA) for 15 lutjanid species in the Caribbean. Specimens were obtained from local catches at La Parguera, Puerto Real and Rincon, western Puerto Rico and Catano, northern Puerto Rico. DNA variation was quantified through the use of polymerase-chain-reaction (PCR) amplification of fragments corresponding to 450 bp of the mtDNA 12S rRNA gene followed by sequencing. Intraspecific variation was not found within any species for the adult specimens analyzed during this study. Assessment of phylogenetic relationships of species was conducted using Neighbor Joining (NJ) and Bayesian Inference (BI) analyses. Phylogenetic relationships within the subfamily Lutjaninae remained rather unresolved for some species. Nevertheless, our study suggests that even if not strongly supported, the groups found are associated according to morphology, habitat or feeding preferences. In addition, the consistency in the sequence data for each species in this study demonstrates that the 12S rRNA gene is a reliable tool for taxonomic identification within this family. As these sequences constitute a sort of molecular key for all the 15 species of lutjanids studied, those were used to identify unknown samples of lutjanid larvae. Larval specimens were obtained from plankton tows using a 300 mum mesh net. Seven species were identified among the collected larvae: Lutjanus apodus, Lutjanus synagris, Lutjanus analis, Lutjanus griseus, Lutjanus mahogoni, Ocyurus chrysurus and Rhomboplites aurorubens. Identification of these larvae increases our understanding of early larval stages taxonomy. In addition, this information is useful for the design of research leading to the description of spawning, dispersal and recruitment patterns, as well as habitat selection for the species. These analyses are of vital relevance for assessments regarding the establishment of Marine Protected Areas (MPAs) as a management option to restore diminishing stocks of fish populations.
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