語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
The role of muscle fiber types in mo...
~
Purdue University., Animal Sciences.
FindBook
Google Book
Amazon
博客來
The role of muscle fiber types in modulating lean growth.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
The role of muscle fiber types in modulating lean growth./
作者:
Zeng, Caiyun.
面頁冊數:
181 p.
附註:
Advisers: David E. Gerrard; Alan Grant.
Contained By:
Dissertation Abstracts International69-10B.
標題:
Agriculture, Animal Culture and Nutrition. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3334073
ISBN:
9780549857549
The role of muscle fiber types in modulating lean growth.
Zeng, Caiyun.
The role of muscle fiber types in modulating lean growth.
- 181 p.
Advisers: David E. Gerrard; Alan Grant.
Thesis (Ph.D.)--Purdue University, 2008.
The overall objective of this research was to investigate the role of muscle fiber types in modulating skeletal muscle growth and development. In the first study, we used MyHC IIX- and IIB- null mice to examine clenbuterol-induced skeletal muscle growth. Four-week-old mice were administered with clenbuterol (20 parts per million in drinking water) for 2 weeks. In soleus muscles, which normally contain no IIX or IIB fibers, the hypertrophic effect of clenbuterol was not observed in any genotypes. Further study demonstrated that clenbuterol induced new fiber type formation only in soleus of wild type mice (IIX and IIB fibers) and IIB null (IIX fibers) mice, not in IIX null mice, suggesting that transitional expression of type IIB MyHC depends on the presence of IIX MyHC. Meanwhile, Clenbuterol induced hypertrophy was blunted in fast muscles (gastrocnemius, TA and EDL) of IIX or IIB null mice, demonstrating that the presence of IIX and IIB muscle fibers is essential for clenbuterol-induced skeletal muscle hypertrophy. Finally, the loss of total fiber number contributed to the reduced muscle growth in IIB null mice and clenbuterol failed to rescue this fiber loss. In the second study, we demonstrated that extensor digitorum longus (EDL) and tibialis anterior (TA) muscles in both wild type and IIB null have the same number of muscle fibers at birth, suggesting that loss of IIB MyHC gene does not alter prenatal muscle fiber formation. Although the gastrocnemius muscles in both wild type and IIB null mice have the same amount of muscle fibers at age of 2 weeks postnatal, the gastrocnemius muscle loses almost 50% of total fiber number by 4 weeks of age postnatal and maintains the existing muscle fiber until adult period of life. In the third study, we hypothesized that skeletal muscle specific overexpression of peroxisome proliferator-activated receptor gammar coactivator 1 alpha (PGC-1alpha) might rescue loss of total fiber number and subsequent muscle mass by driving formation of slower muscle fibers and/or maintaining integrate neuromuscular junction in gastrocnemius muscle of IIB null mice. The proportion of IIA fibers was increased by 18% at the expense of proportion of IIX fibers when PGC-1alpha was overexpressed in gastrocnemius of IIB null mice. Moreover, PGC-1alpha significantly increased total fiber number by 43% in gastrocnemius of IIB null mice. Taken together, IIB muscle fiber is necessary to maintain total fiber number and the subsequent muscle growth, and skeletal muscle specific overexpression of PGC-1alpha partially rescue the loss of total fiber number in gastrocnemius of IIB null mice. These data reveal new insights into understanding the mechanisms controlling postnatal maintenance of total fiber number in skeletal muscles.
ISBN: 9780549857549Subjects--Topical Terms:
1017857
Agriculture, Animal Culture and Nutrition.
The role of muscle fiber types in modulating lean growth.
LDR
:03769nmm 2200301 a 45
001
863265
005
20100721
008
100721s2008 ||||||||||||||||| ||eng d
020
$a
9780549857549
035
$a
(UMI)AAI3334073
035
$a
AAI3334073
040
$a
UMI
$c
UMI
100
1
$a
Zeng, Caiyun.
$3
1031171
245
1 4
$a
The role of muscle fiber types in modulating lean growth.
300
$a
181 p.
500
$a
Advisers: David E. Gerrard; Alan Grant.
500
$a
Source: Dissertation Abstracts International, Volume: 69-10, Section: B, page: .
502
$a
Thesis (Ph.D.)--Purdue University, 2008.
520
$a
The overall objective of this research was to investigate the role of muscle fiber types in modulating skeletal muscle growth and development. In the first study, we used MyHC IIX- and IIB- null mice to examine clenbuterol-induced skeletal muscle growth. Four-week-old mice were administered with clenbuterol (20 parts per million in drinking water) for 2 weeks. In soleus muscles, which normally contain no IIX or IIB fibers, the hypertrophic effect of clenbuterol was not observed in any genotypes. Further study demonstrated that clenbuterol induced new fiber type formation only in soleus of wild type mice (IIX and IIB fibers) and IIB null (IIX fibers) mice, not in IIX null mice, suggesting that transitional expression of type IIB MyHC depends on the presence of IIX MyHC. Meanwhile, Clenbuterol induced hypertrophy was blunted in fast muscles (gastrocnemius, TA and EDL) of IIX or IIB null mice, demonstrating that the presence of IIX and IIB muscle fibers is essential for clenbuterol-induced skeletal muscle hypertrophy. Finally, the loss of total fiber number contributed to the reduced muscle growth in IIB null mice and clenbuterol failed to rescue this fiber loss. In the second study, we demonstrated that extensor digitorum longus (EDL) and tibialis anterior (TA) muscles in both wild type and IIB null have the same number of muscle fibers at birth, suggesting that loss of IIB MyHC gene does not alter prenatal muscle fiber formation. Although the gastrocnemius muscles in both wild type and IIB null mice have the same amount of muscle fibers at age of 2 weeks postnatal, the gastrocnemius muscle loses almost 50% of total fiber number by 4 weeks of age postnatal and maintains the existing muscle fiber until adult period of life. In the third study, we hypothesized that skeletal muscle specific overexpression of peroxisome proliferator-activated receptor gammar coactivator 1 alpha (PGC-1alpha) might rescue loss of total fiber number and subsequent muscle mass by driving formation of slower muscle fibers and/or maintaining integrate neuromuscular junction in gastrocnemius muscle of IIB null mice. The proportion of IIA fibers was increased by 18% at the expense of proportion of IIX fibers when PGC-1alpha was overexpressed in gastrocnemius of IIB null mice. Moreover, PGC-1alpha significantly increased total fiber number by 43% in gastrocnemius of IIB null mice. Taken together, IIB muscle fiber is necessary to maintain total fiber number and the subsequent muscle growth, and skeletal muscle specific overexpression of PGC-1alpha partially rescue the loss of total fiber number in gastrocnemius of IIB null mice. These data reveal new insights into understanding the mechanisms controlling postnatal maintenance of total fiber number in skeletal muscles.
590
$a
School code: 0183.
650
4
$a
Agriculture, Animal Culture and Nutrition.
$3
1017857
690
$a
0475
710
2
$a
Purdue University.
$b
Animal Sciences.
$3
1031160
773
0
$t
Dissertation Abstracts International
$g
69-10B.
790
$a
0183
790
1 0
$a
Gerrard, David E.,
$e
advisor
790
1 0
$a
Grant, Alan,
$e
advisor
790
1 0
$a
Hannon, Kevin
$e
committee member
790
1 0
$a
Spurlock, Michael
$e
committee member
791
$a
Ph.D.
792
$a
2008
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3334073
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9076642
電子資源
11.線上閱覽_V
電子書
EB W9076642
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入