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Viral encapsulation of emulsion and ...

University of California, Los Angeles.

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Viral encapsulation of emulsion and nanoemulsion droplets.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Viral encapsulation of emulsion and nanoemulsion droplets./
作者:	Wilking, Connie Chang.
面頁冊數:	89 p.
附註:	Adviser: Thomas G. Mason.
Contained By:	Dissertation Abstracts International70-03B.
標題:	Biology, Virology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3350555
ISBN:	9781109057690

Viral encapsulation of emulsion and nanoemulsion droplets.
Wilking, Connie Chang.

Viral encapsulation of emulsion and nanoemulsion droplets. - 89 p.

Adviser: Thomas G. Mason.

Thesis (Ph.D.)--University of California, Los Angeles, 2008.

Cowpea Chlorotic Mottle Virus (CCMV) is a spherical plant virus having a diameter of 28 nm. The virus has a capsid, or shell of 180 identical coat proteins, that surrounds single-stranded RNA inside. In this dissertation, we report the first demonstration of the self-assembly of viral coat proteins around nano- and micro-scale emulsion droplets to form capsules, or containers that can mimic the native virus. In native viruses, the viral coat protein serves as a barrier to protect the virus' interior contents, the genomic RNA or DNA, necessary for replication. We have discovered that the CCMV protein, under a wide range of pH and ionic strength conditions, can also encapsulate emulsion droplets. We further demonstrate that coating emulsion droplets with viral protein can be accomplished with various outcomes, including emulsions encapsulated by a single layer of protein, a double layer, or multiple layers. We find evidence of ordered packing of the protein on the surfaces as well as defects, indicative of the inability of the protein to anneal on the surface of the droplets, thereby forming in a manner that involves some aspects of colloidal self-assembly as well as non-equilibrium jamming. We can employ these self-assembled viral containers for the drug-delivery of hydrophobic substances. Since viruses have the ability to readily penetrate specific cells, it may be possible to target delivery of particular drugs to certain cells simply by tailoring the type of viral coating on the surface of the droplets. Thus, the creation of virally encapsulated emulsion droplets can potentially enhance selective targeting and cell penetration, delivering the contents of droplets loaded with hydrophobic drug molecules.

ISBN: 9781109057690Subjects--Topical Terms:

1019068
Biology, Virology.

Viral encapsulation of emulsion and nanoemulsion droplets.
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520 $a Cowpea Chlorotic Mottle Virus (CCMV) is a spherical plant virus having a diameter of 28 nm. The virus has a capsid, or shell of 180 identical coat proteins, that surrounds single-stranded RNA inside. In this dissertation, we report the first demonstration of the self-assembly of viral coat proteins around nano- and micro-scale emulsion droplets to form capsules, or containers that can mimic the native virus. In native viruses, the viral coat protein serves as a barrier to protect the virus' interior contents, the genomic RNA or DNA, necessary for replication. We have discovered that the CCMV protein, under a wide range of pH and ionic strength conditions, can also encapsulate emulsion droplets. We further demonstrate that coating emulsion droplets with viral protein can be accomplished with various outcomes, including emulsions encapsulated by a single layer of protein, a double layer, or multiple layers. We find evidence of ordered packing of the protein on the surfaces as well as defects, indicative of the inability of the protein to anneal on the surface of the droplets, thereby forming in a manner that involves some aspects of colloidal self-assembly as well as non-equilibrium jamming. We can employ these self-assembled viral containers for the drug-delivery of hydrophobic substances. Since viruses have the ability to readily penetrate specific cells, it may be possible to target delivery of particular drugs to certain cells simply by tailoring the type of viral coating on the surface of the droplets. Thus, the creation of virally encapsulated emulsion droplets can potentially enhance selective targeting and cell penetration, delivering the contents of droplets loaded with hydrophobic drug molecules.
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