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Factors influencing survival of bovi...
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Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa./
作者:
Delgado Callisaya, Pedro Angel.
面頁冊數:
56 p.
附註:
Source: Masters Abstracts International, Volume: 47-03, page: 1470.
Contained By:
Masters Abstracts International47-03.
標題:
Agriculture, Animal Culture and Nutrition. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=1461453
ISBN:
9780549961802
Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa.
Delgado Callisaya, Pedro Angel.
Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa.
- 56 p.
Source: Masters Abstracts International, Volume: 47-03, page: 1470.
Thesis (M.S.)--University of Arkansas, 2008.
Numerous factors have been attributed to altering the sex ratio of offspring, but the mechanisms by which these factors could alter sex ratio are unknown. Currently, modified flow cytometry is the only proven method for sorting X- from Y-bearing sperm. However, this technique is not widely available, is expensive, and is not practical in some species where the typical insemination dose is billions of sperm. If a natural skewing occurs in some ejaculates, then identifying those ejaculates and possibly predicting their occurrence would be of great, practical benefit. The most efficient use of semen with a skewed ratio of either X- or Y-bearing sperm would be fresh extension and storage so that reduced insemination doses could be used. Therefore, the experiments were designed to: (1) determine the ratio of X- to Y-chromosome bearing spermatozoa in individual ejaculates of semen and the relationship of any skewed sex ratio with routine morphological evaluation and computer assisted sperm analysis; (2) evaluate sperm viability over time, after dilution and refrigerator storage of fresh semen extended in either synthetic cauda epididymal plasma (CEP2), or in a low in sodium medium (CJ2) and supplemented with either AlbuMAX or egg yolk. To confirm that the ratio of X- to Y-chromosome bearing spermatozoa varies among individual ejaculates of semen, semen was collected for 6 beef bulls weekly for 6 consecutive weeks. Sperm DNA was recovered and quantitative real-time PCR was used to determine the ratio of X- to Y-bearing sperm in each ejaculate. Differences were found in the portion of X-bearing sperm among ejaculates from 4 of the 6 bulls evaluated. In some bulls, significant negative correlations were found between the percentage of X-bearing sperm in an ejaculate and overall, progressive and rapid motility, and sperm track speed. Morphological assessment suggested that the percent live sperm and sperm defects may be negatively correlated with the portion of X-bearing sperm in an ejaculate. In the second study, semen was collected weekly from 4 bulls and assigned within bulls, across treatments. After extension in either CEP2 or CJ2 containing either egg yolk or AlbuMAX, semen was cooled to 4°C, then evaluated for 7 days, using a computer assisted sperm analysis (CASA) system. Total and progressive motility of spermatozoa in CEP2 and CJ2-AlbuMAX were similar (P=0.85 and P=0.23 respectively), but both were lower (P<0.01) when compared to CJ2-yolk. Fewer spermatozoa had rapid motility in CEP2 and CJ2-AlbuMAX compared to CJ2-yolk (P<0.01). Spermatozoa straightness and linearity were greater in CJ2-AlbuMAX and in CJ2-yolk than in CEP2 (P<0.01). The mean velocity (VAP) was greater (P<0.01) in CJ2-AlbuMAX than either CEP2 and CJ2-yolk. The calculated linear velocity (VSL) of spermatozoa in CJ-2-AlbuMAX was superior (P<0.01) to either CJ2-yolk or CEP2 treatment. The calculated curvilinear velocity (VCL) of spermatozoa in CEP2 was lower than CJ2-AlbuMAX (P=0.01), but it was similar with CJ2-yolk (P=0.54). The mean width of sperm head oscillation (ALH) in CEP2 was statistically similar to CJ2-AlbuMAX and CJ2-yolk (P=0.95 and P=0.69 respectively). The number of lateral oscillatory movements of the sperm head around the mean trajectory (BCF) was higher in CJ2-yolk extender compared with CJ2-AlbuMAX and CEP2 extenders (P<0.01). Spermatozoa in CJ2-AlbuMAX had higher BCF than in CEP2 (P<0.01). Overall, every sperm parameter measured by the CASA system was equal to or higher for spermatozoa stored 7 days in CJ2 medium as compared to CEP2. The CJ2 medium supplemented with egg yolk is a viable alternative to other semen extenders for storage of fresh semen.
ISBN: 9780549961802Subjects--Topical Terms:
1017857
Agriculture, Animal Culture and Nutrition.
Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa.
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Numerous factors have been attributed to altering the sex ratio of offspring, but the mechanisms by which these factors could alter sex ratio are unknown. Currently, modified flow cytometry is the only proven method for sorting X- from Y-bearing sperm. However, this technique is not widely available, is expensive, and is not practical in some species where the typical insemination dose is billions of sperm. If a natural skewing occurs in some ejaculates, then identifying those ejaculates and possibly predicting their occurrence would be of great, practical benefit. The most efficient use of semen with a skewed ratio of either X- or Y-bearing sperm would be fresh extension and storage so that reduced insemination doses could be used. Therefore, the experiments were designed to: (1) determine the ratio of X- to Y-chromosome bearing spermatozoa in individual ejaculates of semen and the relationship of any skewed sex ratio with routine morphological evaluation and computer assisted sperm analysis; (2) evaluate sperm viability over time, after dilution and refrigerator storage of fresh semen extended in either synthetic cauda epididymal plasma (CEP2), or in a low in sodium medium (CJ2) and supplemented with either AlbuMAX or egg yolk. To confirm that the ratio of X- to Y-chromosome bearing spermatozoa varies among individual ejaculates of semen, semen was collected for 6 beef bulls weekly for 6 consecutive weeks. Sperm DNA was recovered and quantitative real-time PCR was used to determine the ratio of X- to Y-bearing sperm in each ejaculate. Differences were found in the portion of X-bearing sperm among ejaculates from 4 of the 6 bulls evaluated. In some bulls, significant negative correlations were found between the percentage of X-bearing sperm in an ejaculate and overall, progressive and rapid motility, and sperm track speed. Morphological assessment suggested that the percent live sperm and sperm defects may be negatively correlated with the portion of X-bearing sperm in an ejaculate. In the second study, semen was collected weekly from 4 bulls and assigned within bulls, across treatments. After extension in either CEP2 or CJ2 containing either egg yolk or AlbuMAX, semen was cooled to 4°C, then evaluated for 7 days, using a computer assisted sperm analysis (CASA) system. Total and progressive motility of spermatozoa in CEP2 and CJ2-AlbuMAX were similar (P=0.85 and P=0.23 respectively), but both were lower (P<0.01) when compared to CJ2-yolk. Fewer spermatozoa had rapid motility in CEP2 and CJ2-AlbuMAX compared to CJ2-yolk (P<0.01). Spermatozoa straightness and linearity were greater in CJ2-AlbuMAX and in CJ2-yolk than in CEP2 (P<0.01). The mean velocity (VAP) was greater (P<0.01) in CJ2-AlbuMAX than either CEP2 and CJ2-yolk. The calculated linear velocity (VSL) of spermatozoa in CJ-2-AlbuMAX was superior (P<0.01) to either CJ2-yolk or CEP2 treatment. The calculated curvilinear velocity (VCL) of spermatozoa in CEP2 was lower than CJ2-AlbuMAX (P=0.01), but it was similar with CJ2-yolk (P=0.54). The mean width of sperm head oscillation (ALH) in CEP2 was statistically similar to CJ2-AlbuMAX and CJ2-yolk (P=0.95 and P=0.69 respectively). The number of lateral oscillatory movements of the sperm head around the mean trajectory (BCF) was higher in CJ2-yolk extender compared with CJ2-AlbuMAX and CEP2 extenders (P<0.01). Spermatozoa in CJ2-AlbuMAX had higher BCF than in CEP2 (P<0.01). Overall, every sperm parameter measured by the CASA system was equal to or higher for spermatozoa stored 7 days in CJ2 medium as compared to CEP2. The CJ2 medium supplemented with egg yolk is a viable alternative to other semen extenders for storage of fresh semen.
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