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Porcine embryogenesis and effects of...
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Mississippi State University., Animal and Dairy Sciences.
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Porcine embryogenesis and effects of mycotoxins on early pig development.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Porcine embryogenesis and effects of mycotoxins on early pig development./
作者:
Wang, Hongfeng.
面頁冊數:
89 p.
附註:
Adviser: Erdogan Memili.
Contained By:
Masters Abstracts International47-01.
標題:
Agriculture, Animal Culture and Nutrition. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=1457887
ISBN:
9780549714019
Porcine embryogenesis and effects of mycotoxins on early pig development.
Wang, Hongfeng.
Porcine embryogenesis and effects of mycotoxins on early pig development.
- 89 p.
Adviser: Erdogan Memili.
Thesis (M.S.)--Mississippi State University, 2008.
The effect of culture media and mycotoxin on porcine preimplantation embryonic development has been investigated. In the first experiment, NCSU-23 significantly decreased porcine embryonic cleavage and blastocyst rates compared to the PZM-3 medium. BAX and BCL2L1 transcription levels were similar in blastocysts cultured in both media. Porcine embryonic cleavage rates were significantly decreased in the presence of cycloheximide, and both alpha-amanitin and cycloheximide completely inhibited blastocyst formation. In the mycotoxin experiment, porcine embryonic cleavage rates decreased in 10 muM alpha-ZEA group, while blastocyst rates decreased in 30mu M alpha-ZEA group (P<0.05). Total cell numbers of blastocysts decreased in the 10 muM alpha-ZEA group (P<0.05). The transcription levels of POU5F1 and BCL2L1 were similar, while that of BAX and the BAX/BCL2L1 ratio significantly increased in 3 muM and 10 muM alpha-ZEA groups compared to the control group.
ISBN: 9780549714019Subjects--Topical Terms:
1017857
Agriculture, Animal Culture and Nutrition.
Porcine embryogenesis and effects of mycotoxins on early pig development.
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The effect of culture media and mycotoxin on porcine preimplantation embryonic development has been investigated. In the first experiment, NCSU-23 significantly decreased porcine embryonic cleavage and blastocyst rates compared to the PZM-3 medium. BAX and BCL2L1 transcription levels were similar in blastocysts cultured in both media. Porcine embryonic cleavage rates were significantly decreased in the presence of cycloheximide, and both alpha-amanitin and cycloheximide completely inhibited blastocyst formation. In the mycotoxin experiment, porcine embryonic cleavage rates decreased in 10 muM alpha-ZEA group, while blastocyst rates decreased in 30mu M alpha-ZEA group (P<0.05). Total cell numbers of blastocysts decreased in the 10 muM alpha-ZEA group (P<0.05). The transcription levels of POU5F1 and BCL2L1 were similar, while that of BAX and the BAX/BCL2L1 ratio significantly increased in 3 muM and 10 muM alpha-ZEA groups compared to the control group.
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