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Relationships between motility, fert...

Clemson University.

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Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity./
Author:	Mook, Jennifer Leigh.
Description:	151 p.
Notes:	Adviser: Ashby B. Bodine, II.
Contained By:	Dissertation Abstracts International68-03B.
Subject:	Biology, Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3256194

Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity.
Mook, Jennifer Leigh.

Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity. - 151 p.

Adviser: Ashby B. Bodine, II.

Thesis (Ph.D.)--Clemson University, 2007.

This research had 3 goals. First, differences between subjective and objective motility analysis was determined using bull semen samples. Total motility (MOT) of bull semen samples (N=117) was measured three ways: subjective analysis manually on a microscope and objective analysis on 2 different computer-assisted semen analysis (CASA) systems: Hamilton-Thorne IVOS and Minitube SpermVision (SV). CASA analysis also measures progressive motility (PRO) and related kinematic parameters. Bull semen motility analysis indicated a difference (p<0.05) between average MOT: 83.06 (IVOS), 75.84 (manual), 65.48 (SV), and PRO: 44.25 (IVOS) and 60.07(SV). There were positive relationships (p<0.0001) between manual MOT and both IVOS MOT (r=0.32) and SV MOT (r=0.59). The relationship between IVOS and SV MOT and PRO was also notable (r=0.70 for both MOT and PRO).Subjects--Topical Terms:

1017816
Biology, Physiology.

Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity.
LDR:04153nam 2200277 a 45 001 862103
005 20100720
008 100720s2007 ||||||||||||||||| ||eng d
035 $a (UMI)AAI3256194
035 $a AAI3256194
040 $a UMI $c UMI
100 1 $a Mook, Jennifer Leigh. $3 1029877
245 1 0 $a Relationships between motility, fertility and cellular characteristics of spermatozoa, and the ability of platelet-activating factor to influence porcine productivity.
300 $a 151 p.
500 $a Adviser: Ashby B. Bodine, II.
500 $a Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1484.
502 $a Thesis (Ph.D.)--Clemson University, 2007.
520 $a This research had 3 goals. First, differences between subjective and objective motility analysis was determined using bull semen samples. Total motility (MOT) of bull semen samples (N=117) was measured three ways: subjective analysis manually on a microscope and objective analysis on 2 different computer-assisted semen analysis (CASA) systems: Hamilton-Thorne IVOS and Minitube SpermVision (SV). CASA analysis also measures progressive motility (PRO) and related kinematic parameters. Bull semen motility analysis indicated a difference (p<0.05) between average MOT: 83.06 (IVOS), 75.84 (manual), 65.48 (SV), and PRO: 44.25 (IVOS) and 60.07(SV). There were positive relationships (p<0.0001) between manual MOT and both IVOS MOT (r=0.32) and SV MOT (r=0.59). The relationship between IVOS and SV MOT and PRO was also notable (r=0.70 for both MOT and PRO).
520 $a Second, the ability of platelet-activating factor (PAF) to improve litter survival parameters and fertilization/embryonic death rates were assessed by adding PAF (1x10-7M/mL) to extended high/low fertility boar semen prior to artificial insemination of gilts synchronized with MATRIX(TM). Gilts either farrowed (N=67) or were slaughtered at day 30 of gestation (N=28). PAF treatment of boar semen did not improve litter productivity parameters. Notable variation in litter parameters was observed with low fertility semen use. Gilt weight at time of insemination and boar fertility were the primary factors influencing litter parameters and fertilization/embryonic death rates. Semen motility did not have an effect on litter parameters. Season did not impact litter parameters when high fertility semen was used, but use of low fertility semen in the spring resulted in lower (p<0.05) litter sizes.
520 $a Third, two cellular features that have been associated with fertility, PAF receptor (PAFr) and ubiquitin (UBI), were quantitatively measured in formaldehyde-fixed samples via fluorescence on a FACScan Analyzer (Becton Dickinson) in bull (N=95) and boar (N=26) semen samples to evaluate their relationship to motility and pig litter parameters. Boar semen motility was measured on an IVOS. Analysis of cellular parameters showed that increasing UBI was inversely related to CASA MOT and PRO of bull but not pig samples. Increasing PAFr was associated with reductions in bull but not pig PRO. There was no discernable relationship between UBI and boar fertility, but there was a positive relationship (p<0.05) between UBI in samples not washed with a modified Tyrode's albumin/lactate/pyruvate (TALP) solution prior to fixation and litter parameters: number born total (r=0.55), number born alive (r=0.59), average litter weight (r=0.62), and average wean weight (r=0.60). Washing boar samples with TALP increased measurable levels of PAFr and UBI fluorescence (p<0.05). The data indicate that UBI may be related to features associated with boar fertility, but more work is needed to elucidate the effect of washing on overall fluorescence. Treatment of boar semen with PAF did not impact gilt productivity as expected, but future work needs to evaluate treatment concentrations and interaction of PAF with extender components.
590 $a School code: 0050.
650 4 $a Biology, Physiology. $3 1017816
690 $a 0719
710 2 $a Clemson University. $3 997173
773 0 $t Dissertation Abstracts International $g 68-03B.
790 $a 0050
790 1 0 $a Bodine, Ashby B., II, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3256194