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Fli-1 in B cell proliferation and sy...

Medical University of South Carolina.

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Fli-1 in B cell proliferation and systemic lupus erythematosus.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Fli-1 in B cell proliferation and systemic lupus erythematosus./
作者:	Bradshaw, Sarah Gail.
面頁冊數:	102 p.
附註:	Adviser: Gary Gilkeson.
Contained By:	Dissertation Abstracts International69-02B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3303305
ISBN:	9780549496779

Fli-1 in B cell proliferation and systemic lupus erythematosus.
Bradshaw, Sarah Gail.

Fli-1 in B cell proliferation and systemic lupus erythematosus. - 102 p.

Adviser: Gary Gilkeson.

Thesis (Ph.D.)--Medical University of South Carolina, 2008.

PBMC from patients with SLE and splenic lymphocytes from murine models of SLE demonstrate increased expression of the ETS transcription factor Fli-1. Transgenic overexpression of Fli-1 in normal mice leads to SLE-like disease. These findings suggest investigation of the role of Fli-1 in SLE may elucidate etiology of the disease and potential therapies. The MRL/lpr mouse is a model of SLE exhibiting increased lymphocytic expression of Fli-1. Reduction of Fli-1 expression by heterozygousity at the Fli-1 locus (Fli-1+/-) results in decreased auto- and alloantibodies, proteinuria, and renal pathology; and drastically decreased mortality. Disruption of the C-terminal transactivation domain (Fli-1 DeltaCTA/DeltaCTA, reduces the ability of Fli-1 to activate target genes, but does not affect expression or DNA-binding. Effect of Fli-1 deficiency on B cell proliferation was evaluated in three models--- Fli-1+/-MRL/lpr, Fli-1 +/- C57BL/6, and Fli-1DeltaCTA/DeltaCTA C57BL/6 mice. Proliferative responses of naive B cells from Fli-1-deficient mice to anti-1gM, a combination of PMA and ionomycin, CpG DNA, or LPS were significantly reduced compared to those of Fli-1-wild-type mice of similar strain. Expression of corresponding mitogen receptors was also evaluated. Surface expression of BCR components IgM and Igalpha, TLR4, and TLR9, and intracellular expression of TLR9 was only slightly and inconsistently reduced in naive B cells of Fli-1-deficient mice compared to Fli-1-wild-type mice of similar strain. Transcript expression of several TLR and BCR signaling-related genes was also evaluated. IL12a transcripts were upregulated in Fli-1-deficient MRL/lpr B cells. Transcription of the gene encoding the transcription factor NFAT, a known activator of proliferation, was downregulated in Fli-1-deficient MRL/lpr B cells. The results of these studies clearly demonstrate that Fli-1 deficiency affects B cell proliferative responses to mitogens, independent of BCR and TLR expression. Also, IL12a and NFAT were identified as potentially regulated by Fli-1. Based on published evidence, the differential expression of these genes may dampen proliferative responses of B cells to mitogens. Ultimately, this may be a mechanism by which overexpression of Fli-1 contributes to B cell hyperactivity and subsequent SLE pathogenesis.

ISBN: 9780549496779Subjects--Topical Terms:

1017719
Biology, Molecular.

Fli-1 in B cell proliferation and systemic lupus erythematosus.
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