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A polymeric micro total analysis sys...

University of California, Irvine.

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A polymeric micro total analysis system for single-cell analysis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	A polymeric micro total analysis system for single-cell analysis./
作者:	Lai, Hsuan-Hong.
面頁冊數:	140 p.
附註:	Adviser: Guann Pyng Li.
Contained By:	Dissertation Abstracts International69-01B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3296267
ISBN:	9780549410959

A polymeric micro total analysis system for single-cell analysis.
Lai, Hsuan-Hong.

A polymeric micro total analysis system for single-cell analysis. - 140 p.

Adviser: Guann Pyng Li.

Thesis (Ph.D.)--University of California, Irvine, 2008.

The advancement of microengineering has enabled the manipulation and analysis of single cells, which is critical in understanding the molecular mechanisms underlying the basic physiological functions from the point of view of modern biologists. Unfortunately, analysis of single cells remains challenging from a technical perspective, mainly because of the miniature nature of the cell and the high throughput requirements of the analysis. Lab-on-a-chip (LOC) emerges as a research field that shows great promise in this perspective.

ISBN: 9780549410959Subjects--Topical Terms:

586156
Chemistry, Analytical.

A polymeric micro total analysis system for single-cell analysis.
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245 1 2 $a A polymeric micro total analysis system for single-cell analysis.
300 $a 140 p.
500 $a Adviser: Guann Pyng Li.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0467.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2008.
520 $a The advancement of microengineering has enabled the manipulation and analysis of single cells, which is critical in understanding the molecular mechanisms underlying the basic physiological functions from the point of view of modern biologists. Unfortunately, analysis of single cells remains challenging from a technical perspective, mainly because of the miniature nature of the cell and the high throughput requirements of the analysis. Lab-on-a-chip (LOC) emerges as a research field that shows great promise in this perspective.
520 $a We have demonstrated a micro total analysis system (mu-TAS) combining chip-based electrophoretic separation, fluorescence detection, and a pulsed Nd:YAG laser cell lysis system, in a Poly(dimethylsiloxane) (PDMS) microfluidic analytical platform for the implementation of single-cell analysis. To accomplish the task, a polymeric microfluidic device was fabricated and UV graft polymerization surface modification techniques were used. To optimize the conditions for the surface treatment techniques, the modified surfaces of PDMS were characterized using AIR-IR spectrum and sessile water drop contact angle measurements, and in-channel surfaces were characterized by their electroosmotic flow mobility.
520 $a Accurate single-cell analysis relies on rapid cell lysis and therefore an optical measure of fast cell lysis was implemented and optimized in a microscopic station. The influences of pulse energy and the location of the laser beam with respect to the cell in the microchannel were explored. The observation from the cell disruption experiments suggested that the cell lysis was enabled mainly via a thermo-mechanical instead of a plasma-mediated mechanism.
520 $a Finally, after chip-based electrophoresis and a laser-induced fluorescence (LIF) detection system were incorporated with the laser lysis system in a microfluidic analytical station, a feasibility demonstration of single-cell analysis was implemented. The analytical platform exhibited the capability of fluidic transportation, optical lysis of single cells, separation, and analysis of the lysates by electrophoresis and LIF detection. In comparison with the control experiment, the migration times of the fluorescent signals for the cytosolic fluorophores were in good agreement with those for the standard fluorophores, which confirmed the feasibility of the analytical processes.
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791 $a Ph.D.
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