東華大學圖書館 |

Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes./
作者:	Wang, Weizheng.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	185 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
Contained By:	Dissertations Abstracts International85-06B.
標題:	Chemistry. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30818163
ISBN:	9798381174298

Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes.
Wang, Weizheng.

Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 185 p.

Source: Dissertations Abstracts International, Volume: 85-06, Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2023.

Colorimetric detection methods have attracted increasing attention recently and are now used for routine analysis in different areas due to their unique properties, including friendly expense, easy operation, miniaturization, fast results, and portability. Since antibiotics exhibit complex chemical structures and small sizes in the agri-food system and environment with low concentrations, developing a sensing system for antibiotics detection remains a considerable challenge, such as complicated sample pre-treatment requirements, sophisticated instruments, and highly qualified personnel that boost the detection cost. Moreover, after analysis, most sensing systems can only detect a certain antibiotic residue in the resource which may further reduce the detection efficiency. Hence, researchers have contributed to the multiple bio-recognizer and transducers investigations and studies.Titanium carbide (Ti3C2Tx), known as MXene, is currently among the most attractive 2- dimensional (2D) nanomaterials worldwide for a wide range of applications involving (bio)- sensors, energy storage devices, clinical medication, pharmaceutical industries, etc., owing to their superior properties. This nanomaterial is found to mimic the catalytical behavior of horseradish peroxidase (HRP) in this dissertation that can catalyze the conversion of the enzymatic substrate to their final products and turns the solution color from white, transparent colorless to the corresponding color. Thanks to this technology, the goal of visual detection of multiple targets could come true.In this dissertation, a sensing system was developed based on Ti3C2Tx MXene and its derived hierarchical nanostructured enzyme nano-mimic known as nanozymes that can be used for colorimetric detection of different antibiotic residues in food materials.To develop the multi-antibiotic sensing system, we first started to synthesize the few-layered-Ti3C2Tx MXene (OFL-Ti-MN) that was selectively etched its bulky ceramic (MAX phase) by using the minimally intensive layer delamination (MILD) method. The fabricated OFL-Ti-MN was first explored with its enzymatic nano-mimic behavior by using a typical enzymatic system of hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB) under optimal reaction condition. It can facilitate to trigger of the redox reaction between two substrates (H2O2/TMB) and help turn the white-transparent solution bluish-green. The affinity of OFL-Ti-MN against TMB is higher than that of HRP. On the contrary, the affinity of OFL-Ti-MN for H2O2 is much lower, resulting from the partial oxidation of fabrication OFL-Ti-MN and the vacancy defect of the OFL-Ti-MN Ti surface. However, the color change was inhibited as kanamycin (KAN) and tetracycline (TC) were introduced into this system (OFL-Ti-MN/TMB/H2O2). Based on this strategy, an OFL-Ti-MN-based colorimetric method for KAN and TC detection was successfully architected, showing the limit of detection (LOD) of 15.28 nM and 615.28 nM for KAN and TC, respectively. The exploration of intrinsic peroxidase-mimic properties of OFL-Ti-MN uncovered the plausible mechanism of the inhibition effect of KAN and TC on color change. This provides an alternative way to detect antibiotics and opens the door to discovering the antibiotic function mechanism and extending the OFL-Ti-MN application (Chapters III & IV).A hierarchical nanostructure of Au nanoparticle (Au NPs) doped layered Ti3C2Tx MXene (L L-Ti3C2Tx NS) (MA2) was designed and synthesized via a facile one-step Au precursor (HAuCl4{acute}{80}{Ostrok}3H2O) was reduced directly on the surface of L-Ti3C2Tx NS without adding extra reducing agents. The final MA2 nanocomposite shows the catalase-like properties in the TMB/H2O2 system, with a significantly higher experimental affinity (~ 17 folds) to H2O2 than that of OFL-Ti-MN due to the synergic effect of Au NPs and L-Ti3C2Tx NS. When ampicillin (AMP){A0}or penicillin G (Pen G) exists in the catalase nano-mimic system (MA2/TMB/H2O2), the bluish-green color does not appear, providing a great opportunity to manufacture a colorimetric method for AMP and Pen G detection. As a result, this sensing method showed LOD for AMP and Pen G detection as 7.51 nM and 68.9 nM, respectively, with a great linear relationship of 116.82 nM ~ 3.51 {phono}{aelig}M and 35.1 nM ~ 584 nM for AMP and Pen G, respectively. The proposed mechanism indicates that the MA2 can mimic the catalytic action of natural catalase in the presence of H2O2. Moreover, this further extended the investigation and application of MXene and MXene-based nanocomposites (Chapter V).{A0}

ISBN: 9798381174298Subjects--Topical Terms:

516420
Chemistry.
Subjects--Index Terms:

Antibiotics

Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes.
LDR:05780nmm a2200385 4500 001 2403625
005 20241118135853.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798381174298
035 $a (MiAaPQ)AAI30818163
035 $a AAI30818163
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wang, Weizheng. $3 3286300
245 1 0 $a Colorimetric Sensing of Antibiotic Residues in Foods With MXene and MXene-Based Nanozymes.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 185 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
500 $a Advisor: Gunasekaran, Sundaram.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2023.
520 $a Colorimetric detection methods have attracted increasing attention recently and are now used for routine analysis in different areas due to their unique properties, including friendly expense, easy operation, miniaturization, fast results, and portability. Since antibiotics exhibit complex chemical structures and small sizes in the agri-food system and environment with low concentrations, developing a sensing system for antibiotics detection remains a considerable challenge, such as complicated sample pre-treatment requirements, sophisticated instruments, and highly qualified personnel that boost the detection cost. Moreover, after analysis, most sensing systems can only detect a certain antibiotic residue in the resource which may further reduce the detection efficiency. Hence, researchers have contributed to the multiple bio-recognizer and transducers investigations and studies.Titanium carbide (Ti3C2Tx), known as MXene, is currently among the most attractive 2- dimensional (2D) nanomaterials worldwide for a wide range of applications involving (bio)- sensors, energy storage devices, clinical medication, pharmaceutical industries, etc., owing to their superior properties. This nanomaterial is found to mimic the catalytical behavior of horseradish peroxidase (HRP) in this dissertation that can catalyze the conversion of the enzymatic substrate to their final products and turns the solution color from white, transparent colorless to the corresponding color. Thanks to this technology, the goal of visual detection of multiple targets could come true.In this dissertation, a sensing system was developed based on Ti3C2Tx MXene and its derived hierarchical nanostructured enzyme nano-mimic known as nanozymes that can be used for colorimetric detection of different antibiotic residues in food materials.To develop the multi-antibiotic sensing system, we first started to synthesize the few-layered-Ti3C2Tx MXene (OFL-Ti-MN) that was selectively etched its bulky ceramic (MAX phase) by using the minimally intensive layer delamination (MILD) method. The fabricated OFL-Ti-MN was first explored with its enzymatic nano-mimic behavior by using a typical enzymatic system of hydrogen peroxide (H2O2) and 3,3',5,5'-tetramethylbenzidine (TMB) under optimal reaction condition. It can facilitate to trigger of the redox reaction between two substrates (H2O2/TMB) and help turn the white-transparent solution bluish-green. The affinity of OFL-Ti-MN against TMB is higher than that of HRP. On the contrary, the affinity of OFL-Ti-MN for H2O2 is much lower, resulting from the partial oxidation of fabrication OFL-Ti-MN and the vacancy defect of the OFL-Ti-MN Ti surface. However, the color change was inhibited as kanamycin (KAN) and tetracycline (TC) were introduced into this system (OFL-Ti-MN/TMB/H2O2). Based on this strategy, an OFL-Ti-MN-based colorimetric method for KAN and TC detection was successfully architected, showing the limit of detection (LOD) of 15.28 nM and 615.28 nM for KAN and TC, respectively. The exploration of intrinsic peroxidase-mimic properties of OFL-Ti-MN uncovered the plausible mechanism of the inhibition effect of KAN and TC on color change. This provides an alternative way to detect antibiotics and opens the door to discovering the antibiotic function mechanism and extending the OFL-Ti-MN application (Chapters III & IV).A hierarchical nanostructure of Au nanoparticle (Au NPs) doped layered Ti3C2Tx MXene (L L-Ti3C2Tx NS) (MA2) was designed and synthesized via a facile one-step Au precursor (HAuCl4{acute}{80}{Ostrok}3H2O) was reduced directly on the surface of L-Ti3C2Tx NS without adding extra reducing agents. The final MA2 nanocomposite shows the catalase-like properties in the TMB/H2O2 system, with a significantly higher experimental affinity (~ 17 folds) to H2O2 than that of OFL-Ti-MN due to the synergic effect of Au NPs and L-Ti3C2Tx NS. When ampicillin (AMP){A0}or penicillin G (Pen G) exists in the catalase nano-mimic system (MA2/TMB/H2O2), the bluish-green color does not appear, providing a great opportunity to manufacture a colorimetric method for AMP and Pen G detection. As a result, this sensing method showed LOD for AMP and Pen G detection as 7.51 nM and 68.9 nM, respectively, with a great linear relationship of 116.82 nM ~ 3.51 {phono}{aelig}M and 35.1 nM ~ 584 nM for AMP and Pen G, respectively. The proposed mechanism indicates that the MA2 can mimic the catalytic action of natural catalase in the presence of H2O2. Moreover, this further extended the investigation and application of MXene and MXene-based nanocomposites (Chapter V).{A0}
590 $a School code: 0262.
650 4 $a Chemistry. $3 516420
650 4 $a Food science. $3 3173303
650 4 $a Materials science. $3 543314
650 4 $a Nanoscience. $3 587832
653 $a Antibiotics
653 $a Colorimetric biosensor
653 $a MXene
653 $a Nanozyme
690 $a 0485
690 $a 0794
690 $a 0359
690 $a 0565
710 2 $a The University of Wisconsin - Madison. $b Biological Systems Engineering. $3 2049925
773 0 $t Dissertations Abstracts International $g 85-06B.
790 $a 0262
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30818163