東華大學圖書館 |

Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis./
作者:	Lopez Dauphin, Nahyr Arelis.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	206 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-08, Section: B.
Contained By:	Dissertations Abstracts International84-08B.
標題:	Analytical chemistry. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30246580
ISBN:	9798371976024

Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis.
Lopez Dauphin, Nahyr Arelis.

Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 206 p.

Source: Dissertations Abstracts International, Volume: 84-08, Section: B.

Thesis (Ph.D.)--State University of New York at Buffalo, 2023.

Advances in liquid chromatography (LC) are highly connected with the development of chromatographic column technology. Herein, fibrous silica particles (FSPs) with a diameter of 1.0 μm were investigated as a potential new support material for LC column technology. Their inherent high surface area may provide a means to increase sample loadability. FSPs were derivatized with n-octyltrimethoxy silane (C8-TMS) to evaluate their chromatographic characteristics under reversed-phase (RP) conditions. The particles were examined via FTIR spectroscopy, gas adsorption analysis, thermogravimetric analysis, and scanning electron microscopy before and after functionalization with C8-TMS. In addition, LC columns were packed with functionalized and bare FSPs to evaluate their chromatographic performance using high-performance liquid chromatography (HPLC). Columns packed with bare FSPs exhibited the behavior that is consistent with hydrophilic interaction liquid chromatography (HILIC), while the C8-TMS-functionalized FSPs showed RP characteristics. The separation of probe compounds in a column packed with FSPs deteriorated over time as it was exposed to high pressures. Morphological analysis of the packing material by scanning electron microscopy (SEM) after exposing the packed column to high pressures (up to 12,000 psi) showed the breakage of the particles, suggesting that the 1 μm diameter FSPs are not mechanical stable under the high-pressure condition used in HPLC. In a second study, the adsorptive characteristics of porous and fibrous silica particles functionalized with fluorophilic, and anion-exchange moieties were examined for the adsorption of per and polyfluoroalkyl substances (PFAS) to enhance detectability in LC mass spectrometry (MS) for potential application in the analysis of PFAS in the environment. Fibrous silica particles, as well as traditional porous silica particles, were reacted with different silanes such as aminopropyltriethoxysilane (APTES) and tridecafluoro-1,1,2,2 tetrahydrooctyl triethoxy silane (C8-TFT), resulting in surface coverages of 1.96 and 1.34 μmol/m2 for the porous silica, and 4.43 and 3.32 μmol/m2 respectively for the fibrous silica particles. Dispersive-solid phase extraction (D-SPE) experiments showed the adsorption of long and short-chain PFAS using the modified particles. Several studies, such as loading capacity, adsorption, preliminary elution, and partition coefficient experiments, were performed to understand the behavior and adsorbent capabilities. The loading capacity and adsorption experiments using 10 mg of the fibrous particles modified to contain both C8-TFT and APTES showed the adsorption of perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), heptafluorobutyric acid (HFBA), and hexafluoropropylene oxide-dimer acid (Gen-X) in the range of 10 to 25000 ng/L. Elution experiments revealed that PFOS was extracted completely with ACN. In contrast, the best elution solvent for PFOA, HFBA, and Gen-X was 75:25 acetonitrile/water. Lastly, preliminary studies have shown that a human-derived biofluid (i.e., ascites) has antibacterial activity against Acinetobacter baumannii. However, the compound(s) responsible for the antibacterial activity and the complete profile of this fluid remains unknown. An HPLC method was used to enhance the separation of highly polar components in an ascites biofluid. Previous work on the ascitic fluid had shown antibacterial activity of two isolated early eluting HPLC peaks.The separation method was modified by extending the column length, resulting in higher resolution, which allowed the separation of new species from the early eluting bands. These were fractionated into four different fractions, and their antibacterial activities against A. baumannii were tested; however, the separated species did not show antibacterial properties. In addition, analysis of the ascites fluid via capillary electrophoresis revealed the amount of acetate and formate present in the sample, 0.74 and 4.34 mM. Efforts continue to further separate and identify the different components in the ascitic fluid.

ISBN: 9798371976024Subjects--Topical Terms:

3168300
Analytical chemistry.
Subjects--Index Terms:

Electrophoresis

Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis.
LDR:05312nmm a2200361 4500 001 2401339
005 20241022112551.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798371976024
035 $a (MiAaPQ)AAI30246580
035 $a AAI30246580
035 $a 2401339
040 $a MiAaPQ $c MiAaPQ
100 1 $a Lopez Dauphin, Nahyr Arelis. $0 (orcid)0000-0002-5570-7260 $3 3771430
245 1 0 $a Evaluation of Fibrous Silica Particles for Chromatographic Applications and the Analysis of Human-Derived Biofluids by HPLC and Capillary Electrophoresis.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 206 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-08, Section: B.
500 $a Advisor: Colon, Luis A.
502 $a Thesis (Ph.D.)--State University of New York at Buffalo, 2023.
520 $a Advances in liquid chromatography (LC) are highly connected with the development of chromatographic column technology. Herein, fibrous silica particles (FSPs) with a diameter of 1.0 μm were investigated as a potential new support material for LC column technology. Their inherent high surface area may provide a means to increase sample loadability. FSPs were derivatized with n-octyltrimethoxy silane (C8-TMS) to evaluate their chromatographic characteristics under reversed-phase (RP) conditions. The particles were examined via FTIR spectroscopy, gas adsorption analysis, thermogravimetric analysis, and scanning electron microscopy before and after functionalization with C8-TMS. In addition, LC columns were packed with functionalized and bare FSPs to evaluate their chromatographic performance using high-performance liquid chromatography (HPLC). Columns packed with bare FSPs exhibited the behavior that is consistent with hydrophilic interaction liquid chromatography (HILIC), while the C8-TMS-functionalized FSPs showed RP characteristics. The separation of probe compounds in a column packed with FSPs deteriorated over time as it was exposed to high pressures. Morphological analysis of the packing material by scanning electron microscopy (SEM) after exposing the packed column to high pressures (up to 12,000 psi) showed the breakage of the particles, suggesting that the 1 μm diameter FSPs are not mechanical stable under the high-pressure condition used in HPLC. In a second study, the adsorptive characteristics of porous and fibrous silica particles functionalized with fluorophilic, and anion-exchange moieties were examined for the adsorption of per and polyfluoroalkyl substances (PFAS) to enhance detectability in LC mass spectrometry (MS) for potential application in the analysis of PFAS in the environment. Fibrous silica particles, as well as traditional porous silica particles, were reacted with different silanes such as aminopropyltriethoxysilane (APTES) and tridecafluoro-1,1,2,2 tetrahydrooctyl triethoxy silane (C8-TFT), resulting in surface coverages of 1.96 and 1.34 μmol/m2 for the porous silica, and 4.43 and 3.32 μmol/m2 respectively for the fibrous silica particles. Dispersive-solid phase extraction (D-SPE) experiments showed the adsorption of long and short-chain PFAS using the modified particles. Several studies, such as loading capacity, adsorption, preliminary elution, and partition coefficient experiments, were performed to understand the behavior and adsorbent capabilities. The loading capacity and adsorption experiments using 10 mg of the fibrous particles modified to contain both C8-TFT and APTES showed the adsorption of perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), heptafluorobutyric acid (HFBA), and hexafluoropropylene oxide-dimer acid (Gen-X) in the range of 10 to 25000 ng/L. Elution experiments revealed that PFOS was extracted completely with ACN. In contrast, the best elution solvent for PFOA, HFBA, and Gen-X was 75:25 acetonitrile/water. Lastly, preliminary studies have shown that a human-derived biofluid (i.e., ascites) has antibacterial activity against Acinetobacter baumannii. However, the compound(s) responsible for the antibacterial activity and the complete profile of this fluid remains unknown. An HPLC method was used to enhance the separation of highly polar components in an ascites biofluid. Previous work on the ascitic fluid had shown antibacterial activity of two isolated early eluting HPLC peaks.The separation method was modified by extending the column length, resulting in higher resolution, which allowed the separation of new species from the early eluting bands. These were fractionated into four different fractions, and their antibacterial activities against A. baumannii were tested; however, the separated species did not show antibacterial properties. In addition, analysis of the ascites fluid via capillary electrophoresis revealed the amount of acetate and formate present in the sample, 0.74 and 4.34 mM. Efforts continue to further separate and identify the different components in the ascitic fluid.
590 $a School code: 0656.
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Environmental studies. $3 2122803
653 $a Electrophoresis
653 $a Polyfluoroalkyl substances
653 $a Silica
690 $a 0486
690 $a 0477
710 2 $a State University of New York at Buffalo. $b Chemistry. $3 1035962
773 0 $t Dissertations Abstracts International $g 84-08B.
790 $a 0656
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30246580