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The Role of Glutamate in the Medial ...

Nisanov, Rudolf.

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The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach./
Author:	Nisanov, Rudolf.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2024,
Description:	130 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
Contained By:	Dissertations Abstracts International85-07B.
Subject:	Behavioral sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30989082
ISBN:	9798381429169

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.
Nisanov, Rudolf.

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach. - Ann Arbor : ProQuest Dissertations & Theses, 2024 - 130 p.

Source: Dissertations Abstracts International, Volume: 85-07, Section: B.

Thesis (Ph.D.)--City University of New York, 2024.

This item must not be sold to any third party vendors.

A series of experiments were conducted to assess the role of glutamatergic stimulation in the dorsal and ventral regions of the medial prefrontal cortex (mPFC) in both the acquisition and expression of reward-related learning using a Pavlovian conditioning paradigm, the conditioned-approach paradigm. Rats with surgically implanted cannulas were exposed to Pavlovian conditioning sessions that occurred on three alternative days (acquisition) or seven consecutive days (expression). These 60-min conditioning sessions consisted of 30 pairings of light (CS) and food pellets (US) presented under a random time schedule. After a two-day break, rats underwent a session with no CS or US and a CS-only (extinction) test session on the following day. For the acquisition of conditioned approach, NMDA receptors were blocked with AP5 prior to each of the three conditioning sessions. For expression, AMPA receptors were blocked with CNQX prior to the CS-only test session. The results from the acquisition experiments show substantially greater responding for the CS (conditioned approach) in rats with inhibited NMDA receptors in both regions of mPFC in comparison to control groups. In the expression experiments, however, inhibition of AMPA receptors in the dorsal region resulted in significant{A0}dose-dependent impairment of conditioned approach but in the ventral region it resulted in dose-dependent facilitation of conditioned approach in comparison to the respective control groups. An RNAscope was employed to assess whether the CS - a food-paired light stimulus -activated more neurons than the light stimulus not explicitly paired with food (neutral stimulus). The results revealed that the CS resulted in significantly higher neuronal activation than the non-CS and that the neuronal population that was affected were glutamatergic neurons. The final experiment used chemogenetics to inhibit glutamatergic projections from dmPFC to NAc, finding a significant impairment in conditioned approach expression. Confocal microscopy revealed the pathway originating from dmPFC glutamatergic cells to NAc, supporting the role of top-down executive control in learned behavior expression.

ISBN: 9798381429169Subjects--Topical Terms:

529833
Behavioral sciences.
Subjects--Index Terms:

Conditioned approach

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.
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300 $a 130 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
500 $a Advisor: Ranaldi, Robert.
502 $a Thesis (Ph.D.)--City University of New York, 2024.
506 $a This item must not be sold to any third party vendors.
520 $a A series of experiments were conducted to assess the role of glutamatergic stimulation in the dorsal and ventral regions of the medial prefrontal cortex (mPFC) in both the acquisition and expression of reward-related learning using a Pavlovian conditioning paradigm, the conditioned-approach paradigm. Rats with surgically implanted cannulas were exposed to Pavlovian conditioning sessions that occurred on three alternative days (acquisition) or seven consecutive days (expression). These 60-min conditioning sessions consisted of 30 pairings of light (CS) and food pellets (US) presented under a random time schedule. After a two-day break, rats underwent a session with no CS or US and a CS-only (extinction) test session on the following day. For the acquisition of conditioned approach, NMDA receptors were blocked with AP5 prior to each of the three conditioning sessions. For expression, AMPA receptors were blocked with CNQX prior to the CS-only test session. The results from the acquisition experiments show substantially greater responding for the CS (conditioned approach) in rats with inhibited NMDA receptors in both regions of mPFC in comparison to control groups. In the expression experiments, however, inhibition of AMPA receptors in the dorsal region resulted in significant{A0}dose-dependent impairment of conditioned approach but in the ventral region it resulted in dose-dependent facilitation of conditioned approach in comparison to the respective control groups. An RNAscope was employed to assess whether the CS - a food-paired light stimulus -activated more neurons than the light stimulus not explicitly paired with food (neutral stimulus). The results revealed that the CS resulted in significantly higher neuronal activation than the non-CS and that the neuronal population that was affected were glutamatergic neurons. The final experiment used chemogenetics to inhibit glutamatergic projections from dmPFC to NAc, finding a significant impairment in conditioned approach expression. Confocal microscopy revealed the pathway originating from dmPFC glutamatergic cells to NAc, supporting the role of top-down executive control in learned behavior expression.
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