東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies./
作者:	Rohner, Eduarde.
面頁冊數:	1 online resource (60 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
標題:	Medical research. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29316541click for full text (PQDT)
ISBN:	9798351490830

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
Rohner, Eduarde.

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies. - 1 online resource (60 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--Karolinska Institutet (Sweden), 2022.

Includes bibliographical references

In the heart, phospholamban (PLN) is a key regulator of calcium cycling through itsinhibitory effect on the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. For morethan two decades, modulating calcium channels has been a strong focus of prospectivecardiac therapeutics with a recent emphasis on targeting PLN itself, partially due to theprogressive uncovering of several cardiomyopathic PLN human mutations. Ourunderstanding of the physiological and pathophysiological mechanisms regulating ormisregulating calcium cycling in humans in not fully understood. Therefore, careful study ofnaturally occurring human mutations can offer important insights into these mechanisms.This thesis focuses on developing novel investigative and potential therapeutic tools tomechanically dissect and treat phospholamban mediated cardiomyopathies.The constituent papers compiled in this thesis validates these novel and innovativeapproaches which utilize an mRNA modular system to model and dissect core mechanisticdifferences caused by cardiomyopathic phospholamban mutations in embryonic stem cellderived cardiomyocytes. Additionally, this thesis showcases the usage of single-chain VHHantibodies (intrabodies) to express, target and modulate PLN intracellularly in vitro and in vivo with promising results validating its usage as a potential therapy and valuableinvestigative tool.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798351490830Subjects--Topical Terms:

1556686
Medical research.
Index Terms--Genre/Form:

542853
Electronic books.

A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
LDR:02681nmm a2200337K 4500 001 2365690
005 20231218204638.5
006 m o d
007 cr mn ---uuuuu
008 241011s2022 xx obm 000 0 eng d
020 $a 9798351490830
035 $a (MiAaPQ)AAI29316541
035 $a (MiAaPQ)Karolinska_1061648091
035 $a AAI29316541
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Rohner, Eduarde. $3 3706554
245 1 2 $a A Modular MRNA Approach to Study and Treat Phospholamban Mediated Cardiomyopathies.
264 0 $c 2022
300 $a 1 online resource (60 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
500 $a Advisor: Chien, Kenneth R. ; Sahara, Makoto ; Santoro, Federica ; Louch, William E.
502 $a Thesis (Ph.D.)--Karolinska Institutet (Sweden), 2022.
504 $a Includes bibliographical references
520 $a In the heart, phospholamban (PLN) is a key regulator of calcium cycling through itsinhibitory effect on the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. For morethan two decades, modulating calcium channels has been a strong focus of prospectivecardiac therapeutics with a recent emphasis on targeting PLN itself, partially due to theprogressive uncovering of several cardiomyopathic PLN human mutations. Ourunderstanding of the physiological and pathophysiological mechanisms regulating ormisregulating calcium cycling in humans in not fully understood. Therefore, careful study ofnaturally occurring human mutations can offer important insights into these mechanisms.This thesis focuses on developing novel investigative and potential therapeutic tools tomechanically dissect and treat phospholamban mediated cardiomyopathies.The constituent papers compiled in this thesis validates these novel and innovativeapproaches which utilize an mRNA modular system to model and dissect core mechanisticdifferences caused by cardiomyopathic phospholamban mutations in embryonic stem cellderived cardiomyocytes. Additionally, this thesis showcases the usage of single-chain VHHantibodies (intrabodies) to express, target and modulate PLN intracellularly in vitro and in vivo with promising results validating its usage as a potential therapy and valuableinvestigative tool.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Medical research. $2 bicssc $3 1556686
650 4 $a Physiology. $3 518431
650 4 $a Medicine. $3 641104
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0564
690 $a 0719
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Karolinska Institutet (Sweden). $3 3557748
773 0 $t Dissertations Abstracts International $g 84-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29316541 $z click for full text (PQDT)