東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility./
作者:	Chappell, Timothy Michael.
面頁冊數:	1 online resource (223 pages)
附註:	Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
Contained By:	Dissertations Abstracts International85-03B.
標題:	Plant sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30316190click for full text (PQDT)
ISBN:	9798380161695

Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility.
Chappell, Timothy Michael.

Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility. - 1 online resource (223 pages)

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

Thesis (Ph.D.)--University of Georgia, 2023.

Includes bibliographical references

Soybean is one of the most widely grown crops in the world. The advent of genetic engineering in soybean has allowed genetic manipulation of soybean for a variety of modifications. Development of transgenic soybean plants is primarily through the use of particle bombardment to deliver DNA. This is because soybean is recalcitrant to the most commonly used method for genetic engineering, Agrobacterium-mediated transformation. Resistance to the microbe Agrobacterium by almost all soybean genotypes implicates the soybean immune system. A single line has been previously identified as extremely susceptible to Agrobacterium, the soybean accession Peking, identified as PI 548402. This accession is important for understanding how Agrobacterium susceptibility is controlled in most soybean varieties, thus opening the door to genetic engineering of more soybean varieties. Chapter one contains a brief history of soybeans in plant breeding and plant immunity, with a focus on previous work characterizing Agrobacterium and soybean interactions. Chapter two describes an identified candidate gene for susceptibility in soybean and subsequent testing with Agrobacterium, modified strains, and candidate gene knockouts. Chapter three details the development of a mapping population derived from a Peking x Century inbred line and a resistant line, Jack or PI 540556, to determine the regions of the genome responsible for susceptibility, with further analysis of candidate genes identified within this region using RNA-Seq. Collectively, these chapters address finding the genomic regions in soybean that control susceptibility to Agrobacterium, using this knowledge to identify what changes could be implemented in the future to make transformation of all soybean genotypes possible. With these goals in mind, the objective of expanding Agrobacterium susceptibility to previously restricted soybean genotypes can be accomplished.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798380161695Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

AgrobacteriumIndex Terms--Genre/Form:

542853
Electronic books.

Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility.
LDR:03239nmm a2200385K 4500 001 2363176
005 20231116093816.5
006 m o d
007 cr mn ---uuuuu
008 241011s2023 xx obm 000 0 eng d
020 $a 9798380161695
035 $a (MiAaPQ)AAI30316190
035 $a AAI30316190
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Chappell, Timothy Michael. $3 3703925
245 1 0 $a Dissecting the Genetic Basis of Soybean-Agrobacterium Compatibility.
264 0 $c 2023
300 $a 1 online resource (223 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
500 $a Advisor: Parrott, Wayne.
502 $a Thesis (Ph.D.)--University of Georgia, 2023.
504 $a Includes bibliographical references
520 $a Soybean is one of the most widely grown crops in the world. The advent of genetic engineering in soybean has allowed genetic manipulation of soybean for a variety of modifications. Development of transgenic soybean plants is primarily through the use of particle bombardment to deliver DNA. This is because soybean is recalcitrant to the most commonly used method for genetic engineering, Agrobacterium-mediated transformation. Resistance to the microbe Agrobacterium by almost all soybean genotypes implicates the soybean immune system. A single line has been previously identified as extremely susceptible to Agrobacterium, the soybean accession Peking, identified as PI 548402. This accession is important for understanding how Agrobacterium susceptibility is controlled in most soybean varieties, thus opening the door to genetic engineering of more soybean varieties. Chapter one contains a brief history of soybeans in plant breeding and plant immunity, with a focus on previous work characterizing Agrobacterium and soybean interactions. Chapter two describes an identified candidate gene for susceptibility in soybean and subsequent testing with Agrobacterium, modified strains, and candidate gene knockouts. Chapter three details the development of a mapping population derived from a Peking x Century inbred line and a resistant line, Jack or PI 540556, to determine the regions of the genome responsible for susceptibility, with further analysis of candidate genes identified within this region using RNA-Seq. Collectively, these chapters address finding the genomic regions in soybean that control susceptibility to Agrobacterium, using this knowledge to identify what changes could be implemented in the future to make transformation of all soybean genotypes possible. With these goals in mind, the objective of expanding Agrobacterium susceptibility to previously restricted soybean genotypes can be accomplished.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Plant sciences. $3 3173832
650 4 $a Plant pathology. $3 3174872
650 4 $a Molecular biology. $3 517296
650 4 $a Genetics. $3 530508
653 $a Agrobacterium
653 $a Soybean
653 $a Genetic engineering
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0479
690 $a 0480
690 $a 0307
690 $a 0369
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a University of Georgia. $b Plant Breeding, Genetics and Genomics - PHD. $3 3703926
773 0 $t Dissertations Abstracts International $g 85-03B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30316190 $z click for full text (PQDT)