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Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease./
作者:	Srinivasan, Divya.
面頁冊數:	1 online resource (78 pages)
附註:	Source: Masters Abstracts International, Volume: 84-10.
Contained By:	Masters Abstracts International84-10.
標題:	Animal diseases. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30351564click for full text (PQDT)
ISBN:	9798377678502

Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease.
Srinivasan, Divya.

Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease. - 1 online resource (78 pages)

Source: Masters Abstracts International, Volume: 84-10.

Thesis (M.E.)--McGill University (Canada), 2022.

Includes bibliographical references

Newcastle Disease has been causing much economic loss and food security issues in Sub-Saharan Africa. The risks associated with a zoonotic virus outbreak includes animal to human transmission. Vaccines against Newcastle Disease Virus have been in production in Africa, using chicken egg-based methods which are more expensive and have less throughput. There is a need to produce vaccines more efficiently. A recombinant adenovirus vectored NDV vaccine was developed in the earlier phase of the project. The goal of this research endeavor was to develop a more stable formulation for the developed Ad-CMV-F virus. An empirical screening test was performed to identify possible excipients and evaluated with TCID50 assay to determine the infectious titers in formulation after stress testing the virus formulation in temperatures of 4ºC, 37 ºC, and 22 ºC. Excipients that showed a good result was further combined in formulations and compared against each other after exposure at 37 ºC for a week using TCID50 assay and ddPCR. The stability of the virus that was purified with CsCl gradient ultracentrifugation was compared with the clarified product of tangential flow filtration, after exposure to a week of 37 ºC and performing TCID50 assay. It was shown that trehalose 2% and polysorbate-80 in combination were effective as a formulation in maintaining the infectious titers.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798377678502Subjects--Topical Terms:

3181862
Animal diseases.
Index Terms--Genre/Form:

542853
Electronic books.

Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease.
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245 1 0 $a Formulation Development of Recombinant Adenovirus-Based Vaccine for Newcastle Disease.
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300 $a 1 online resource (78 pages)
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500 $a Source: Masters Abstracts International, Volume: 84-10.
500 $a Advisor: Kamen, Amine.
502 $a Thesis (M.E.)--McGill University (Canada), 2022.
504 $a Includes bibliographical references
520 $a Newcastle Disease has been causing much economic loss and food security issues in Sub-Saharan Africa. The risks associated with a zoonotic virus outbreak includes animal to human transmission. Vaccines against Newcastle Disease Virus have been in production in Africa, using chicken egg-based methods which are more expensive and have less throughput. There is a need to produce vaccines more efficiently. A recombinant adenovirus vectored NDV vaccine was developed in the earlier phase of the project. The goal of this research endeavor was to develop a more stable formulation for the developed Ad-CMV-F virus. An empirical screening test was performed to identify possible excipients and evaluated with TCID50 assay to determine the infectious titers in formulation after stress testing the virus formulation in temperatures of 4ºC, 37 ºC, and 22 ºC. Excipients that showed a good result was further combined in formulations and compared against each other after exposure at 37 ºC for a week using TCID50 assay and ddPCR. The stability of the virus that was purified with CsCl gradient ultracentrifugation was compared with the clarified product of tangential flow filtration, after exposure to a week of 37 ºC and performing TCID50 assay. It was shown that trehalose 2% and polysorbate-80 in combination were effective as a formulation in maintaining the infectious titers.
520 $a La maladie de Newcastle est a l'origine de nombreuses pertes economiques et de problemes de securite alimentaire en Afrique subsaharienne. Les risques associes a une epidemie de virus zoonotique comprennent la transmission de l'animal a l'homme. Des vaccins contre le virus de la maladie de Newcastle ont ete produits en Afrique, en utilisant des methodes a base d'oeufs de poule qui sont plus couteuses et ont un rendement moindre. Il est necessaire de produire des vaccins plus efficacement. Un vaccin NDV vectorise par un adenovirus recombinant a ete developpe dans la phase precedente du projet. L'objectif de ce projet de recherche etait de developper une formulation plus stable pour le virus Ad-CMV-F developpe. Un test de criblage empirique a ete effectue pour identifier les excipients possibles, et evalue avec le test TCID50 pour determiner les titres infectieux dans la formulation apres avoir teste la formulation du virus a des temperatures de 4ºC, 37ºC, et 22ºC. Les excipients qui ont donne un bon resultat ont ete combines dans des formulations et compares les uns aux autres apres une exposition a 37 ºC pendant une semaine en utilisant le test TCID50 et la ddPCR. La stabilite du virus qui a ete purifie par ultracentrifugation en gradient de CsCl a ete comparee au produit clarifie de la filtration a flux tangentiel, apres exposition a une semaine a 37 ºC et realisation du test TCID50. Il a ete demontre que le trehalose a 2 % et le polysorbate-80 en combinaison etaient efficaces en tant que formulation pour maintenir les titres infectieux.
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