東華大學圖書館 |

Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement./
作者:	Fenstemaker, Sean Michael.
面頁冊數:	1 online resource (267 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
標題:	Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30013413click for full text (PQDT)
ISBN:	9798351442853

Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement.
Fenstemaker, Sean Michael.

Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement. - 1 online resource (267 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--The Ohio State University, 2021.

Includes bibliographical references

Plant breeders have used wild relatives as a source of genetic diversity for biotic and abiotic stress mitigation since the early 20th century. This natural allelic diversity is a vital resource for crop improvement. The focus of this dissertation was to use tomato as a model to compare two methods of accessing trait diversity from wild relatives: introgression breeding and grafting. The specific aims were to estimate the genetic and environmental contributions to trait delivery methods and assess their relative efficacy and limitations. An accession of the endemic Galapagos tomato, S. galapagense LA1141, provided allelic variation for the genetic dissection of purple fruit pigmentation and tolerance to water deficit stress. Accessions of S. pimpinellifolium and S. habrochaites were used as parents to develop interspecific hybrid rootstock for multi-year, multi-location field trials.The first objective was to determine the chemical and genetic basis of purple pigmentation. Accession LA1141 and a processing tomato, OH8245, were used to develop populations for the simultaneous characterization and introgression of traits. The breeding strategy employed repeated backcrossing (BC) followed by inbreeding (S). The LA1141 x OH8245 populations provided plant materials to identify genetic factors that underlie quantitative trait loci (QTL) while introducing these traits into a commercially viable genetic background. I genotyped the LA1141 x OH8245 BC2S3 generation with single nucleotide polymorphisms, created a linkage map, and conducted composite interval mapping. Anthocyanins were identified as causal pigments, and QTL analysis revealed genetic regions that explained as much as 35% of the variation in color. These analyses led to the identification of candidate genes. Subsequent sequence and phylogenetic analyses supported a conservation of mechanism leading to purple fruit, while identifying novel alleles at the Anthocyanin fruit, atroviolacium, and uniform ripening loci. The second objective was to describe the genetic basis of water deficit stress tolerance derived from LA1141, compare phenotyping methodologies for large germplasm screens, and evaluate selection strategies. A digital phenotyping workflow based on infrared images was developed to assess water deficit stress. QTLs were identified and explained 9% of the phenotypic variation. Advanced lines chosen using phenotypic and genomic selection performed better than randomly advanced lines. The third objective was to determine if I could access tolerance to water deficit stress derived from LA1141 through grafting. Grafting improved plant physiological status but did not maintain yield and quality under deficit irrigation in the greenhouse evaluation. My fourth objective tested the efficacy of grafting to improve cultivated tomatoes in the field by using rootstocks developed from crosses with S. pimpinellifolium and S. habrochaites. Data from these field trials were used to quantify the genetic contributions of the parents used for the development of interspecific hybrid rootstocks. Grafting doubled the yield of a high-lycopene scion while maintaining levels of potentially bioactive carotenoids. This dissertation provides a framework for comparing the relative merits of introgression breeding and grafting as a method of trait delivery from tomato wild relatives as a source of genetic diversity.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798351442853Subjects--Topical Terms:

555447
Horticulture.
Subjects--Index Terms:

Introgression breedingIndex Terms--Genre/Form:

542853
Electronic books.

Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement.
LDR:05115nmm a2200517K 4500 001 2362599
005 20231102121840.5
006 m o d
007 cr mn ---uuuuu
008 241011s2021 xx obm 000 0 eng d
020 $a 9798351442853
035 $a (MiAaPQ)AAI30013413
035 $a AAI30013413
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Fenstemaker, Sean Michael. $3 3703343
245 1 0 $a Using Wild Relatives as a Source of Traits through Introgression Breeding and Grafting for Tomato Improvement.
264 0 $c 2021
300 $a 1 online resource (267 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
500 $a Advisor: Fancis, David.
502 $a Thesis (Ph.D.)--The Ohio State University, 2021.
504 $a Includes bibliographical references
520 $a Plant breeders have used wild relatives as a source of genetic diversity for biotic and abiotic stress mitigation since the early 20th century. This natural allelic diversity is a vital resource for crop improvement. The focus of this dissertation was to use tomato as a model to compare two methods of accessing trait diversity from wild relatives: introgression breeding and grafting. The specific aims were to estimate the genetic and environmental contributions to trait delivery methods and assess their relative efficacy and limitations. An accession of the endemic Galapagos tomato, S. galapagense LA1141, provided allelic variation for the genetic dissection of purple fruit pigmentation and tolerance to water deficit stress. Accessions of S. pimpinellifolium and S. habrochaites were used as parents to develop interspecific hybrid rootstock for multi-year, multi-location field trials.The first objective was to determine the chemical and genetic basis of purple pigmentation. Accession LA1141 and a processing tomato, OH8245, were used to develop populations for the simultaneous characterization and introgression of traits. The breeding strategy employed repeated backcrossing (BC) followed by inbreeding (S). The LA1141 x OH8245 populations provided plant materials to identify genetic factors that underlie quantitative trait loci (QTL) while introducing these traits into a commercially viable genetic background. I genotyped the LA1141 x OH8245 BC2S3 generation with single nucleotide polymorphisms, created a linkage map, and conducted composite interval mapping. Anthocyanins were identified as causal pigments, and QTL analysis revealed genetic regions that explained as much as 35% of the variation in color. These analyses led to the identification of candidate genes. Subsequent sequence and phylogenetic analyses supported a conservation of mechanism leading to purple fruit, while identifying novel alleles at the Anthocyanin fruit, atroviolacium, and uniform ripening loci. The second objective was to describe the genetic basis of water deficit stress tolerance derived from LA1141, compare phenotyping methodologies for large germplasm screens, and evaluate selection strategies. A digital phenotyping workflow based on infrared images was developed to assess water deficit stress. QTLs were identified and explained 9% of the phenotypic variation. Advanced lines chosen using phenotypic and genomic selection performed better than randomly advanced lines. The third objective was to determine if I could access tolerance to water deficit stress derived from LA1141 through grafting. Grafting improved plant physiological status but did not maintain yield and quality under deficit irrigation in the greenhouse evaluation. My fourth objective tested the efficacy of grafting to improve cultivated tomatoes in the field by using rootstocks developed from crosses with S. pimpinellifolium and S. habrochaites. Data from these field trials were used to quantify the genetic contributions of the parents used for the development of interspecific hybrid rootstocks. Grafting doubled the yield of a high-lycopene scion while maintaining levels of potentially bioactive carotenoids. This dissertation provides a framework for comparing the relative merits of introgression breeding and grafting as a method of trait delivery from tomato wild relatives as a source of genetic diversity.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Horticulture. $3 555447
650 4 $a Botany. $3 516217
650 4 $a Plant sciences. $3 3173832
650 4 $a Plant biology. $3 3186449
650 4 $a Agricultural engineering. $3 3168406
650 4 $a Bioengineering. $3 657580
650 4 $a Genetics. $3 530508
653 $a Introgression breeding
653 $a Grafting
653 $a Anthocyanin
653 $a Abiotic stress
653 $a High throughput phenotyping
653 $a Water deficit
653 $a Tomato wilt
653 $a Solanum galapagense
653 $a Solanum pimpinellifolium
653 $a Solanum habroachaites
653 $a Phylogenetics
653 $a Purple tomato
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0479
690 $a 0309
690 $a 0471
690 $a 0202
690 $a 0369
690 $a 0539
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a The Ohio State University. $b Horticulture and Crop Science. $3 1676892
773 0 $t Dissertations Abstracts International $g 84-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30013413 $z click for full text (PQDT)