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Improving Greenhouse Floriculture Crop Quality Using Biotechnology and Beneficial Microbes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Improving Greenhouse Floriculture Crop Quality Using Biotechnology and Beneficial Microbes./
作者:	Lin, Yiyun.
面頁冊數:	1 online resource (247 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
Contained By:	Dissertations Abstracts International84-02B.
標題:	Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29337825click for full text (PQDT)
ISBN:	9798834084693

Improving Greenhouse Floriculture Crop Quality Using Biotechnology and Beneficial Microbes.
Lin, Yiyun.

Improving Greenhouse Floriculture Crop Quality Using Biotechnology and Beneficial Microbes. - 1 online resource (247 pages)

Source: Dissertations Abstracts International, Volume: 84-02, Section: B.

Thesis (Ph.D.)--The Ohio State University, 2021.

Includes bibliographical references

Floriculture plants are economically important crops that benefit the environment and our society. The value of floriculture crops largely depends on the plant quality. To better serve the needs of the fast-developing floriculture industry, novel approaches are needed to enhance crop quality more efficiently and sustainably. The overall goal of this study was to understand and improve the quality of floriculture crops using biotechnology and beneficial microbial inoculants.Flower longevity is a valued quality trait of floriculture crops. The regulation of flower longevity is determined by the programmed cell death (PCD) process in flower petals. Autophagy is one of the crucial regulators of PCD, and it is involved in the regulation of nutrient recycling during petal senescence. To determine the role of autophagy in petal senescence, the expression of two essential autophagy genes Autophagy Gene 6 (PhATG6) and Phosphoinositide 3-Kinase (PhPI3K) were silenced in Petunia x hybrida `Picobella Blue' using Virus-induce Gene Silencing (VIGS). PhATG6- and PhPI3K-silenced plants showed decreased flower longevity, indicating that autophagy plays a negative role in petal senescence regulation. To further understand the function of autophagy during petal senescence, stable transgenic P. hybrida `Mitchell Diploid' plants with knocked-out (KO) PhATG6 were generated using CRISPR/Cas9. Compared to the wild-type controls, the PhATG6-KO lines exhibited decreased flower longevity, earlier production of ethylene, accelerated expression of PCD-related genes, and inefficient nutrient remobilization. These results confirmed the negative regulatory role of PhATG6 in petal senescence and suggested that autophagy modulated petal senescence and nutrient recycling through an ethylene-mediated process. Additionally, VIGS PhATG6 and PhPI3K plants showed reduced flower numbers and decreased plant biomass, and PhATG6-KO lines produced lower seed pod weight, supporting additional functions of autophagy in plant development. Our study provided genetic information for the improvement of floriculture crop quality through biotechnology.An easy-to-implement strategy to improve the quality of floriculture crops is through the application of microbial biostimulants in greenhouse production. Thirteen biostimulant products were evaluated in greenhouse-grown petunia (P. hybrida `Carpet White') and zinnia (Zinnia elegans `Magellan Ivory') produced with lower than optimal fertilizer rates. Three effective biostimulants were found to improve plant growth, health, and quality, as measured by plant size (growth index), leaf chlorophyll content (SPAD index), shoot biomass, root: shoot ratio, leaf size, flower biomass, flower number, and leaf mineral nutrient content. Plants treated with certain biostimulants performed equivalent to or better than the untreated plants grown at optimal fertility. In summary, the application of carefully selected plant biostimulants is an effective and sustainable solution for floriculture crop quality improvement.The sustainable growth of the floriculture industry requires continuous improvements to production practices and floriculture crop quality. Novel strategies and evidence-driven practices should be applied to establish inclusive and collaborative programs for the consistent improvement of floriculture crops. Both plant biotechnology and the application of beneficial microbes have been shown effective in characterizing and enhancing plant quality in this study.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798834084693Subjects--Topical Terms:

555447
Horticulture.
Subjects--Index Terms:

GreenhouseIndex Terms--Genre/Form:

542853
Electronic books.

Improving Greenhouse Floriculture Crop Quality Using Biotechnology and Beneficial Microbes.
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520 $a Floriculture plants are economically important crops that benefit the environment and our society. The value of floriculture crops largely depends on the plant quality. To better serve the needs of the fast-developing floriculture industry, novel approaches are needed to enhance crop quality more efficiently and sustainably. The overall goal of this study was to understand and improve the quality of floriculture crops using biotechnology and beneficial microbial inoculants.Flower longevity is a valued quality trait of floriculture crops. The regulation of flower longevity is determined by the programmed cell death (PCD) process in flower petals. Autophagy is one of the crucial regulators of PCD, and it is involved in the regulation of nutrient recycling during petal senescence. To determine the role of autophagy in petal senescence, the expression of two essential autophagy genes Autophagy Gene 6 (PhATG6) and Phosphoinositide 3-Kinase (PhPI3K) were silenced in Petunia x hybrida `Picobella Blue' using Virus-induce Gene Silencing (VIGS). PhATG6- and PhPI3K-silenced plants showed decreased flower longevity, indicating that autophagy plays a negative role in petal senescence regulation. To further understand the function of autophagy during petal senescence, stable transgenic P. hybrida `Mitchell Diploid' plants with knocked-out (KO) PhATG6 were generated using CRISPR/Cas9. Compared to the wild-type controls, the PhATG6-KO lines exhibited decreased flower longevity, earlier production of ethylene, accelerated expression of PCD-related genes, and inefficient nutrient remobilization. These results confirmed the negative regulatory role of PhATG6 in petal senescence and suggested that autophagy modulated petal senescence and nutrient recycling through an ethylene-mediated process. Additionally, VIGS PhATG6 and PhPI3K plants showed reduced flower numbers and decreased plant biomass, and PhATG6-KO lines produced lower seed pod weight, supporting additional functions of autophagy in plant development. Our study provided genetic information for the improvement of floriculture crop quality through biotechnology.An easy-to-implement strategy to improve the quality of floriculture crops is through the application of microbial biostimulants in greenhouse production. Thirteen biostimulant products were evaluated in greenhouse-grown petunia (P. hybrida `Carpet White') and zinnia (Zinnia elegans `Magellan Ivory') produced with lower than optimal fertilizer rates. Three effective biostimulants were found to improve plant growth, health, and quality, as measured by plant size (growth index), leaf chlorophyll content (SPAD index), shoot biomass, root: shoot ratio, leaf size, flower biomass, flower number, and leaf mineral nutrient content. Plants treated with certain biostimulants performed equivalent to or better than the untreated plants grown at optimal fertility. In summary, the application of carefully selected plant biostimulants is an effective and sustainable solution for floriculture crop quality improvement.The sustainable growth of the floriculture industry requires continuous improvements to production practices and floriculture crop quality. Novel strategies and evidence-driven practices should be applied to establish inclusive and collaborative programs for the consistent improvement of floriculture crops. Both plant biotechnology and the application of beneficial microbes have been shown effective in characterizing and enhancing plant quality in this study.
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