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Selecting for CRISPR Mutagenesis in Tomatoes with Counter Selectable Markers DAAO and HSVtk.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Selecting for CRISPR Mutagenesis in Tomatoes with Counter Selectable Markers DAAO and HSVtk./
作者:	Huang, Kaiwen.
面頁冊數:	1 online resource (78 pages)
附註:	Source: Masters Abstracts International, Volume: 79-07.
Contained By:	Masters Abstracts International79-07.
標題:	Molecular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10623098click for full text (PQDT)
ISBN:	9780355451610

Selecting for CRISPR Mutagenesis in Tomatoes with Counter Selectable Markers DAAO and HSVtk.
Huang, Kaiwen.

Selecting for CRISPR Mutagenesis in Tomatoes with Counter Selectable Markers DAAO and HSVtk. - 1 online resource (78 pages)

Source: Masters Abstracts International, Volume: 79-07.

Thesis (M.S.)--University of California, Davis, 2017.

Includes bibliographical references

With advanced understanding of the recently discovered CRISPR (clustered regularly interspaced short palindromic repeats) /Cas (CRISPR-associated) gene editing system, we are now able to perform more precise editing on desired loci. Nevertheless, current plant genome editing methods often require a tissue culture approach, which usually takes a great amount of time and labor to achieve. In this study, we aimed at utilizing the CRISPR technology to modify the tomato genome, and develop a novel method to select for mutagenesis with counter selectable markers (CSMs), in which plants undergo the processes of mutagenesis, decapitation, and selection, resulting in regeneration of new shoots. The CSM is designed to lead to the regeneration of mutated plants only. We have conducted tests on several potential CSMs and established a feasible way to deliver selecting agents to plants. We have also performed multiplex editing by targeting two genes in tomato, and the result was promising.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780355451610Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

Counter selectionIndex Terms--Genre/Form:

542853
Electronic books.

Selecting for CRISPR Mutagenesis in Tomatoes with Counter Selectable Markers DAAO and HSVtk.
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500 $a Source: Masters Abstracts International, Volume: 79-07.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Britt, Anne B.
502 $a Thesis (M.S.)--University of California, Davis, 2017.
504 $a Includes bibliographical references
520 $a With advanced understanding of the recently discovered CRISPR (clustered regularly interspaced short palindromic repeats) /Cas (CRISPR-associated) gene editing system, we are now able to perform more precise editing on desired loci. Nevertheless, current plant genome editing methods often require a tissue culture approach, which usually takes a great amount of time and labor to achieve. In this study, we aimed at utilizing the CRISPR technology to modify the tomato genome, and develop a novel method to select for mutagenesis with counter selectable markers (CSMs), in which plants undergo the processes of mutagenesis, decapitation, and selection, resulting in regeneration of new shoots. The CSM is designed to lead to the regeneration of mutated plants only. We have conducted tests on several potential CSMs and established a feasible way to deliver selecting agents to plants. We have also performed multiplex editing by targeting two genes in tomato, and the result was promising.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Molecular biology. $3 517296
650 4 $a Botany. $3 516217
650 4 $a Plant sciences. $3 3173832
653 $a Counter selection
653 $a Crispr
653 $a Daao
653 $a Hsvtk
653 $a Multiplex editing
653 $a Tomato
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10623098 $z click for full text (PQDT)