東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation./
作者:	Harden, James Terrell.
面頁冊數:	1 online resource (163 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
標題:	Spleen. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29342278click for full text (PQDT)
ISBN:	9798351496115

Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation.
Harden, James Terrell.

Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation. - 1 online resource (163 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--Stanford University, 2022.

Includes bibliographical references

There's a multitude of functions that our immune system has to carry out in order to sustain the human body. It actively protects us from cancers, diseasecausing microbes, and toxins, all while maintaining tolerance to non-dangerous foreign substances such as pollen and gut bacteria. It also has additional regulatory functions that impact pregnancy, tissue recovery, and wound healing. The dynamic and multi-dimensional properties of our immune system that makes it so remarkable are the same properties that cause us problems in solid-organ transplantation and causes acute rejection. Our immune system views genetically different tissue of the transplanted organ as a dangerous foreign substance similar to a pathogen and attacks it. The ongoing challenge is to determine how we can circumvent the natural defense mechanisms of the immune system without sacrificing its numerous regulatory functions.The datasets described in this thesis address this challenge by applying high-dimensional cytometry analysis to deep profile the peripheral immune cells involved in acute rejection using a murine model of transplantation. More than 40 different markers are used to obtain a highly granular and multi-parametric proteomic overview of the immune signature that defines acute rejection. This analysis is performed in spleen and lymph node over multiple days post-transplant from day 1 to day 7. T cells, NK cells, monocytes, macrophages, and dendritic cells are expansively profiled to ascertain the differences in protein composition that associate with acute rejection. This high resolution characterization of the immune response reveals a unique phenotypic signature of rejection that is evident by day 5 post-transplantation, and clearly distinguishes rejecting from non-rejecting grafts. This alloimmune phenotype is furthermore conserved across two distinct models of acute rejection. Lastly, strategies to prolong allograft survival are investigated with the use of plasmacytoid dendritic cells (pDC). The results reported here reveal tolerogenic properties that are unique to pDC when compared to conventional dendritic cells. The molecular profile of these pDC points to a potential mechanism of harnessing the immune system's natural immune suppressive capabilities to prolong graft survival. Taken together, these data provide a comprehensive and dynamic map of the alloimmune response in solid organ transplant and provides a foundation for unveiling new therapeutic strategies that can effectively delay graft rejection.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798351496115Subjects--Topical Terms:

3561759
Spleen.
Index Terms--Genre/Form:

542853
Electronic books.

Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation.
LDR:03894nmm a2200397K 4500 001 2359817
005 20230917195255.5
006 m o d
007 cr mn ---uuuuu
008 241011s2022 xx obm 000 0 eng d
020 $a 9798351496115
035 $a (MiAaPQ)AAI29342278
035 $a (MiAaPQ)STANFORDct259fz7496
035 $a AAI29342278
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Harden, James Terrell. $3 3700432
245 1 0 $a Establishing a Comprehensive and Dynamic Map of the Alloimmune Response in Solid Organ Transplantation.
264 0 $c 2022
300 $a 1 online resource (163 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
500 $a Advisor: Krams, Sheri.
502 $a Thesis (Ph.D.)--Stanford University, 2022.
504 $a Includes bibliographical references
520 $a There's a multitude of functions that our immune system has to carry out in order to sustain the human body. It actively protects us from cancers, diseasecausing microbes, and toxins, all while maintaining tolerance to non-dangerous foreign substances such as pollen and gut bacteria. It also has additional regulatory functions that impact pregnancy, tissue recovery, and wound healing. The dynamic and multi-dimensional properties of our immune system that makes it so remarkable are the same properties that cause us problems in solid-organ transplantation and causes acute rejection. Our immune system views genetically different tissue of the transplanted organ as a dangerous foreign substance similar to a pathogen and attacks it. The ongoing challenge is to determine how we can circumvent the natural defense mechanisms of the immune system without sacrificing its numerous regulatory functions.The datasets described in this thesis address this challenge by applying high-dimensional cytometry analysis to deep profile the peripheral immune cells involved in acute rejection using a murine model of transplantation. More than 40 different markers are used to obtain a highly granular and multi-parametric proteomic overview of the immune signature that defines acute rejection. This analysis is performed in spleen and lymph node over multiple days post-transplant from day 1 to day 7. T cells, NK cells, monocytes, macrophages, and dendritic cells are expansively profiled to ascertain the differences in protein composition that associate with acute rejection. This high resolution characterization of the immune response reveals a unique phenotypic signature of rejection that is evident by day 5 post-transplantation, and clearly distinguishes rejecting from non-rejecting grafts. This alloimmune phenotype is furthermore conserved across two distinct models of acute rejection. Lastly, strategies to prolong allograft survival are investigated with the use of plasmacytoid dendritic cells (pDC). The results reported here reveal tolerogenic properties that are unique to pDC when compared to conventional dendritic cells. The molecular profile of these pDC points to a potential mechanism of harnessing the immune system's natural immune suppressive capabilities to prolong graft survival. Taken together, these data provide a comprehensive and dynamic map of the alloimmune response in solid organ transplant and provides a foundation for unveiling new therapeutic strategies that can effectively delay graft rejection.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Spleen. $3 3561759
650 4 $a Dendritic cells. $3 1001571
650 4 $a Transplants & implants. $3 3562683
650 4 $a Neutrophils. $3 878084
650 4 $a Antibodies. $3 709277
650 4 $a Cytotoxicity. $3 3682273
650 4 $a Lymphatic system. $3 3685740
650 4 $a Immune system. $3 689864
650 4 $a Cytokines. $3 687114
650 4 $a Lymphocytes. $3 895384
650 4 $a Inflammation. $3 662994
650 4 $a Flow cytometry. $3 600581
650 4 $a Antigens. $3 700737
650 4 $a Algorithms. $3 536374
650 4 $a Clustering. $3 3559215
650 4 $a Heart. $3 578987
650 4 $a Genotype & phenotype. $3 3561790
650 4 $a Protein expression. $3 3554057
650 4 $a Cellular biology. $3 3172791
650 4 $a Computer science. $3 523869
650 4 $a Genetics. $3 530508
650 4 $a Immunology. $3 611031
650 4 $a Medicine. $3 641104
650 4 $a Morphology. $3 591167
650 4 $a Surgery. $3 707153
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0379
690 $a 0984
690 $a 0369
690 $a 0982
690 $a 0564
690 $a 0287
690 $a 0576
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Stanford University. $3 754827
773 0 $t Dissertations Abstracts International $g 84-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29342278 $z click for full text (PQDT)