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Investigating Ultrasound for 3D Biofabrication and Non-Destructive Quality Monitoring of Biomimetic Engineered Tissues.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Investigating Ultrasound for 3D Biofabrication and Non-Destructive Quality Monitoring of Biomimetic Engineered Tissues./
作者:	Asif, Suleman.
面頁冊數:	1 online resource (185 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Contained By:	Dissertations Abstracts International84-12B.
標題:	Tissue engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30463940click for full text (PQDT)
ISBN:	9798379650155

Investigating Ultrasound for 3D Biofabrication and Non-Destructive Quality Monitoring of Biomimetic Engineered Tissues.
Asif, Suleman.

Investigating Ultrasound for 3D Biofabrication and Non-Destructive Quality Monitoring of Biomimetic Engineered Tissues. - 1 online resource (185 pages)

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

Thesis (Ph.D.)--North Carolina State University, 2023.

Includes bibliographical references

The overall goal of this dissertation is to investigate ultrasound for biofabrication and nondestructive quality monitoring of biomimetic engineered tissue constructs to help accelerate the translation of tissue engineering technologies from labs to scalable manufacturing systems, and ultimately, to clinics.In order to engineer functional tissue constructs, it is crucial to develop biofabrication processes that enable cellular and extracellular matrix (ECM) organizations within the constructs to mimic the unique micro-architectural characteristics of native tissues. Recently, an ultrasoundassisted biofabrication (UAB) process was developed that uses acoustic radiation forces from standing bulk acoustic standing waves (SBAW) to preferentially organize cellular arrays within hydrogel constructs. This dissertation investigates UAB for fabrication of composite tissue constructs using biofunctionalized bioinks containing cellular spheroids, and biomaterial additives that provide anchor surfaces for cells to attach and produce anisotropic ECM to mimic anisotropic organization of native tissues.Development of new non-destructive, non-invasive, label-free, and real-time quality monitoring techniques is equally important and necessary to complement the advances in biofabrication processes. Many current assessment methods are destructive, offline, and timeintensive, and typically not suitable for application in scalable tissue manufacturing systems. Ultrasound technologies have the potential to be non-invasive, non-destructive, alternatives. To this end, this dissertation also investigates a quantitative ultrasound (QUS) approach for assessment of biological critical quality attributes (CQA) of engineered tissue constructs.The primary objectives of this dissertation are as follows:1. Understand the process physics of ultrasound-assisted patterning of cellular spheroids, collagen microaggregates, and polycaprolactone (PCL) microfibers in composite bioinks using analytical and computational modeling and experimental validation.2. Experimentally characterize the material-process-structure-function interrelationships in UAB of anisotropic tissue constructs using composite bioinks.3. Characterize the ultrasound propagation characteristics in hydrogels and the relationships between ultrasound attenuation spectroscopy metrics, and cell size and cell concentration using computational modeling and experimental validation.4. Investigate applications of QUS to monitor the functional characteristics of cells in biomimetic tissue engineered constructs in culture over time. The dissertation work has resulted in a validated analytical and computational framework that describes the acoustic pressure distribution and acoustic radiation forces acting on spheroids and bioadditives, and their associated patterning time characteristics in UAB of composite tissue constructs. Outcomes of experimental studies highlight the effects of material and process parameters such as spheroid density, bioadditives concentration, and ultrasound frequency on cellular responses, ECM formation, and mechanical properties of the composite constructs. In parallel, computational models that can accurately predict the cell size and concentration based on attenuation of ultrasound waves traversing the hydrogel constructs have been developed and experimentally validated under different scenarios using a new non-invasive assessment setup. In addition, two different applications of QUS to monitor the functional characteristics of cells in tissue engineered constructs are presented. Ultrasound attenuation spectroscopy was found to be effective in evaluating cell proliferation in anisotropic tissue constructs and detection of system failure and interruptions in the tissue growth process. Ultrasound attenuation was also found to be sensitive to differentiation of stem cells in long-term culture. The knowledge generated in this dissertation will pave the pathway for development of more advanced ultrasound technologies for biofabrication and non-destructive quality assessment of biomimetic engineered tissues.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379650155Subjects--Topical Terms:

823582
Tissue engineering.
Index Terms--Genre/Form:

542853
Electronic books.

Investigating Ultrasound for 3D Biofabrication and Non-Destructive Quality Monitoring of Biomimetic Engineered Tissues.
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