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Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis./
Author:	Keong, Lee Choon.
Description:	1 online resource (230 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
Contained By:	Dissertations Abstracts International84-04B.
Subject:	Nitric oxide. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29352268click for full text (PQDT)
ISBN:	9798352682562

Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis.
Keong, Lee Choon.

Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis. - 1 online resource (230 pages)

Source: Dissertations Abstracts International, Volume: 84-04, Section: B.

Thesis (Ph.D.)--National University of Singapore (Singapore), 2020.

Includes bibliographical references

The delivery of bioactive molecules through the skin remains an attractive route of administration in osteoarthritis (OA) due to the local accumulation at the administration site while reducing systemic side effects. Among the available technologies, a proniosome gel is a suitable dermal delivery platform comprising non-ionic surfactants that self-assemble into de-hydrated vesicles. By exploiting the enhanced skin permeation properties of non-ionic surfactants and by modulating the hydration ability of the proniosome gel, the active ingredients can be efficiently released at the administration site with minimal mechanical force In thisstudy, berberine was used as a model anti-inflammatory compound to demonstrate that a combination of Span® 80 and Tween® 20 in a Span® 60-based proniosome gel led to a high loading of berberine and its successful release from the formulation and into the skin. This was confirmed by advanced in silico simulations as well as through an ex vivo skin permeation study. Concurrently, an in vitro model of OA using primary mouse chondrocytes demonstrated that berberine, at a concentration of 1 µg/mL, was sufficient to restore the production of sulphated glycosaminoglycans (sGAG) to levels comparable to healthy chondrocytes. This amount proved to be below the cytotoxic concentration (IC50 = 33 µg/mL) on skin keratinocytes. In a mouse model of OA, the optimised proniosome gel formulation was consistently able to deliver berberine to exert its pharmacological effects by attenuating inflammation and pain, while minimising cartilage degeneration. Taken together, these data demonstrate the feasibility of adopting proniosome gel as a dermal delivery platform for berberine in managing inflammation and pain in OA.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798352682562Subjects--Topical Terms:

750512
Nitric oxide.
Index Terms--Genre/Form:

542853
Electronic books.

Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis.
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100 1 $a Keong, Lee Choon. $3 3699460
245 1 0 $a Dermal Delivery of Berberine for Suppression of Pain and Inflammation in Knee Osteoarthritis.
264 0 $c 2020
300 $a 1 online resource (230 pages)
336 $a text $b txt $2 rdacontent
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500 $a Source: Dissertations Abstracts International, Volume: 84-04, Section: B.
500 $a Advisor: Pastorin, Giorgia.
502 $a Thesis (Ph.D.)--National University of Singapore (Singapore), 2020.
504 $a Includes bibliographical references
520 $a The delivery of bioactive molecules through the skin remains an attractive route of administration in osteoarthritis (OA) due to the local accumulation at the administration site while reducing systemic side effects. Among the available technologies, a proniosome gel is a suitable dermal delivery platform comprising non-ionic surfactants that self-assemble into de-hydrated vesicles. By exploiting the enhanced skin permeation properties of non-ionic surfactants and by modulating the hydration ability of the proniosome gel, the active ingredients can be efficiently released at the administration site with minimal mechanical force In thisstudy, berberine was used as a model anti-inflammatory compound to demonstrate that a combination of Span® 80 and Tween® 20 in a Span® 60-based proniosome gel led to a high loading of berberine and its successful release from the formulation and into the skin. This was confirmed by advanced in silico simulations as well as through an ex vivo skin permeation study. Concurrently, an in vitro model of OA using primary mouse chondrocytes demonstrated that berberine, at a concentration of 1 µg/mL, was sufficient to restore the production of sulphated glycosaminoglycans (sGAG) to levels comparable to healthy chondrocytes. This amount proved to be below the cytotoxic concentration (IC50 = 33 µg/mL) on skin keratinocytes. In a mouse model of OA, the optimised proniosome gel formulation was consistently able to deliver berberine to exert its pharmacological effects by attenuating inflammation and pain, while minimising cartilage degeneration. Taken together, these data demonstrate the feasibility of adopting proniosome gel as a dermal delivery platform for berberine in managing inflammation and pain in OA.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Nitric oxide. $3 750512
650 4 $a Drug delivery systems. $3 657987
650 4 $a Knee. $3 1364375
650 4 $a Scanning electron microscopy. $3 551366
650 4 $a Chronic illnesses. $3 3550688
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Chemistry. $3 516420
650 4 $a Medicine. $3 641104
650 4 $a Pharmacology. $3 634543
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0486
690 $a 0485
690 $a 0564
690 $a 0419
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a National University of Singapore (Singapore). $3 3352228
773 0 $t Dissertations Abstracts International $g 84-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29352268 $z click for full text (PQDT)