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Vibrationally Sensitive Nonlinear Optical Microscopy with Infrared Light.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Vibrationally Sensitive Nonlinear Optical Microscopy with Infrared Light./
作者:
Hanninen, Adam M.
面頁冊數:
1 online resource (134 pages)
附註:
Source: Dissertations Abstracts International, Volume: 80-09, Section: B.
Contained By:
Dissertations Abstracts International80-09B.
標題:
Biomedical engineering. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13420250click for full text (PQDT)
ISBN:
9781392001837
Vibrationally Sensitive Nonlinear Optical Microscopy with Infrared Light.
Hanninen, Adam M.
Vibrationally Sensitive Nonlinear Optical Microscopy with Infrared Light.
- 1 online resource (134 pages)
Source: Dissertations Abstracts International, Volume: 80-09, Section: B.
Thesis (Ph.D.)--University of California, Irvine, 2018.
Includes bibliographical references
Sum frequency generation microscopy (SFG) is a nonlinear optical technique used to visualize interfaces and noncentrosymmetric structures that lack inversion symmetry. The advantage of SFG, which uses two collinearly coherent lasers over its degenerate counterpart, second harmonic generation (SHG), is that it enables chemical contrast through molecular vibrational absorptions in the infrared known as the fingerprint region. IR-active molecular modes are energized into a vibrationally excited state through a direct dipole excitation, and then probed with higher energy photon(s) before parametrically radiating a photon at the sum of the incident frequencies. Incorporating this technique into a multi-modal microscope is an important development as it allows for high resolution imaging with spectroscopic information without exogenous labels. Following the development of the SFG microscope came the discovery of vibrationally resonant third-order sum frequency generation (TSFG), a four-wave mixing process that probes resonant contributions of the χ(3) tensor. This technique,comparable in sensitivity to the coherent anti-Stokes Raman scattering (CARS) modality,was applied to label-free imaging of cells rich in lipid droplet content. This work contributes to the study of high resolution IR-microscopy by merging it with the field of nonlinear optical microscopy.
Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023
Mode of access: World Wide Web
ISBN: 9781392001837Subjects--Topical Terms:
535387
Biomedical engineering.
Subjects--Index Terms:
FingerprintIndex Terms--Genre/Form:
542853
Electronic books.
Vibrationally Sensitive Nonlinear Optical Microscopy with Infrared Light.
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Sum frequency generation microscopy (SFG) is a nonlinear optical technique used to visualize interfaces and noncentrosymmetric structures that lack inversion symmetry. The advantage of SFG, which uses two collinearly coherent lasers over its degenerate counterpart, second harmonic generation (SHG), is that it enables chemical contrast through molecular vibrational absorptions in the infrared known as the fingerprint region. IR-active molecular modes are energized into a vibrationally excited state through a direct dipole excitation, and then probed with higher energy photon(s) before parametrically radiating a photon at the sum of the incident frequencies. Incorporating this technique into a multi-modal microscope is an important development as it allows for high resolution imaging with spectroscopic information without exogenous labels. Following the development of the SFG microscope came the discovery of vibrationally resonant third-order sum frequency generation (TSFG), a four-wave mixing process that probes resonant contributions of the χ(3) tensor. This technique,comparable in sensitivity to the coherent anti-Stokes Raman scattering (CARS) modality,was applied to label-free imaging of cells rich in lipid droplet content. This work contributes to the study of high resolution IR-microscopy by merging it with the field of nonlinear optical microscopy.
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