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Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology./
Author:	Redford, Glen Ivan.
Description:	1 online resource (239 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 68-01, Section: B.
Contained By:	Dissertations Abstracts International68-01B.
Subject:	Biophysics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3202159click for full text (PQDT)
ISBN:	9780542505690

Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology.
Redford, Glen Ivan.

Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology. - 1 online resource (239 pages)

Source: Dissertations Abstracts International, Volume: 68-01, Section: B.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.

Includes bibliographical references

A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use lifetime imager has proven useful for many applications and essential for several. Examples of applications where such an instrument is needed (and where a conventional system is inadequate) include: any system with large photo-bleaching effects, systems where the intensity changes on the timescale of the measurement, and situations that require real-time display of lifetime images. Measuring photo-porphyrin IX in cells is an example of a system where photo-bleaching makes it difficult to measure. Measuring the lifetime changes in dark adapted plant leaves upon exposure to light is an example of a system with complicated intensity changes. Real-time display of lifetime images is required for clinical work. This paper includes details of the hardware and software for the instrument as well as data taken with the instrument.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780542505690Subjects--Topical Terms:

518360
Biophysics.
Subjects--Index Terms:

Fluorescence lifetimeIndex Terms--Genre/Form:

542853
Electronic books.

Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology.
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245 1 0 $a Fast fluorescence lifetime imaging using a full-field homodyne system with applications in biology.
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500 $a Source: Dissertations Abstracts International, Volume: 68-01, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Clegg, Robert M.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
504 $a Includes bibliographical references
520 $a A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use lifetime imager has proven useful for many applications and essential for several. Examples of applications where such an instrument is needed (and where a conventional system is inadequate) include: any system with large photo-bleaching effects, systems where the intensity changes on the timescale of the measurement, and situations that require real-time display of lifetime images. Measuring photo-porphyrin IX in cells is an example of a system where photo-bleaching makes it difficult to measure. Measuring the lifetime changes in dark adapted plant leaves upon exposure to light is an example of a system with complicated intensity changes. Real-time display of lifetime images is required for clinical work. This paper includes details of the hardware and software for the instrument as well as data taken with the instrument.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
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650 4 $a Optics. $3 517925
653 $a Fluorescence lifetime
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