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Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium./
作者:	Donth, Claudia Maria.
面頁冊數:	1 online resource (265 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-05, Section: B.
Contained By:	Dissertations Abstracts International84-05B.
標題:	Prokaryotes. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29423294click for full text (PQDT)
ISBN:	9798352967119

Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium.
Donth, Claudia Maria.

Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium. - 1 online resource (265 pages)

Source: Dissertations Abstracts International, Volume: 84-05, Section: B.

Thesis (Ph.D.)--Universitaet Bayreuth (Germany), 2022.

Includes bibliographical references

Cells are crowded by a multitude of structures of different sizes. So crowding can be expected to differ significantly depending on the locus within the cell as well as the lengthscale at which it is investigated. This thesis focuses on characterizing intracellular crowding states and crowding heterogeneities on several lengthscales. Living HeLa cells were analysed using fluorescence intensity imaging, ratiometric imaging, ratiometric FRET, FLIM, FLPM and FCS with the fluorescent sensors EGFP, Perceval HR and fCrH2. In addition, fCrH2 was proved to reflect only its surroundings' crowding state. Cytoplasmic crowding levels were found to exceed nucleoplasmic crowding levels, a difference that is retained to some degree after nuclear envelope breakdown. Crowding heterogeneity was found to be more pronounced in the cytoplasm than in the nucleoplasm on intermediate lengthscales, but not on lengthscales below several hundred nanometres. These results were found to be temporally invariant and independent of the existence of a cytoskeleton. Heterogeneity levels in mitotic compartments are very similar on all lengthscales investigated. Exposure to osmolar or oxidative stress as well as ATP depletion was found to influence crowding levels, but not their heterogeneities. A notable exception to this rule is osmolar stress, where intermediate crowding heterogeneity changes as well. The results of this thesis can be used in simulations on intracellular transport, e.g. in medical applications.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798352967119Subjects--Topical Terms:

741052
Prokaryotes.
Index Terms--Genre/Form:

542853
Electronic books.

Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium.
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100 1 $a Donth, Claudia Maria. $3 3695638
245 1 0 $a Heterogeneity of Cellular Compartments Out of Thermodynamic Equilibrium.
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300 $a 1 online resource (265 pages)
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500 $a Source: Dissertations Abstracts International, Volume: 84-05, Section: B.
500 $a Advisor: Weiss, Matthias ; Enders, Axel.
502 $a Thesis (Ph.D.)--Universitaet Bayreuth (Germany), 2022.
504 $a Includes bibliographical references
520 $a Cells are crowded by a multitude of structures of different sizes. So crowding can be expected to differ significantly depending on the locus within the cell as well as the lengthscale at which it is investigated. This thesis focuses on characterizing intracellular crowding states and crowding heterogeneities on several lengthscales. Living HeLa cells were analysed using fluorescence intensity imaging, ratiometric imaging, ratiometric FRET, FLIM, FLPM and FCS with the fluorescent sensors EGFP, Perceval HR and fCrH2. In addition, fCrH2 was proved to reflect only its surroundings' crowding state. Cytoplasmic crowding levels were found to exceed nucleoplasmic crowding levels, a difference that is retained to some degree after nuclear envelope breakdown. Crowding heterogeneity was found to be more pronounced in the cytoplasm than in the nucleoplasm on intermediate lengthscales, but not on lengthscales below several hundred nanometres. These results were found to be temporally invariant and independent of the existence of a cytoskeleton. Heterogeneity levels in mitotic compartments are very similar on all lengthscales investigated. Exposure to osmolar or oxidative stress as well as ATP depletion was found to influence crowding levels, but not their heterogeneities. A notable exception to this rule is osmolar stress, where intermediate crowding heterogeneity changes as well. The results of this thesis can be used in simulations on intracellular transport, e.g. in medical applications.
520 $a Zellen sind dicht gepackte Systeme, die vielfaltige Strukturen unterschiedlicher Grose enthalten. Es ist zu erwarten, dass die lokale Gedrangtheit sich stark unterscheidet, je nachdem, welcher Ort in der Zelle auf welcher Grosenskala betrachtet wird. Ziel dieser Arbeit ist es, die intrazellulare Gedrangtheit sowie deren Heterogenitat auf mehreren Langenskalen zu charakterisieren. Lebende HeLa-Zellen wurden mittels fluoreszenzintensitatsbasierter und ratiometrischer Bildgebung, ratiometrischem FRET, FLIM, FLPM und FCS untersucht. Dabei wurden die Fluoreszenzsensoren EGFP, Perceval HR und fCrH2 verwendet. Bei letzterem wurde zunachst nachgewiesen, dass tatsachlich ausschlieslich die Gedrangtheit der Umgebung wiedergegeben wird. Es wurde gezeigt, dass das Zytoplasma dichter gepackt ist als das Nukleoplasma. Dieser Unterschied wird teilweise auch nach dem Abbau der Kernmembran beibehalten. Die Heterogenitat ist auf mittleren Langenskalen im Zytoplasma starker ausgepragt als im Nukleoplasma. Auf Langenskalen unter einigen hundert Nanometern verschwindet dieser Unterschied. Diese Ergebnisse sind zeitlich invariant und nicht an die Existenz des Zytoskeletts gebunden. In Mitosezellen ahneln sich die Heterogenitaten beider Kompartimente auf allen Langenskalen stark. Die Ausubung von osmolarem oder oxidativem Stress wie auch ATP-Entzug beeinflussen zwar die Gedrangtheit an sich, jedoch nicht deren Heterogenitat. Lediglich unter osmolarem Stress andert sich auch die Heterogenitat der Kompartimente. Die Ergebnisse dieser Arbeit konnen beispielsweise in Simulationen von intrazellularen Transportprozessen verwendet werden und sind daher insbesondere fur medizinische Anwendungen von Interesse.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
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650 4 $a Cytoplasm. $3 3337992
650 4 $a Endoplasmic reticulum. $3 663051
650 4 $a Gene expression. $3 643979
650 4 $a Cytoskeleton. $3 549552
650 4 $a Genetic engineering. $3 530509
650 4 $a Chromosomes. $3 638948
650 4 $a Mitochondria. $3 592563
650 4 $a Cervical cancer. $3 3687017
650 4 $a Genomes. $3 592593
650 4 $a E coli. $3 3556834
650 4 $a Lipids. $3 558980
650 4 $a Expected values. $3 3563993
650 4 $a Cell cycle. $3 766119
650 4 $a Bioengineering. $3 657580
650 4 $a Bioinformatics. $3 553671
650 4 $a Cellular biology. $3 3172791
650 4 $a Genetics. $3 530508
650 4 $a Oncology. $3 751006
650 4 $a Thermodynamics. $3 517304
655 7 $a Electronic books. $2 lcsh $3 542853
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690 $a 0715
690 $a 0379
690 $a 0369
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710 2 $a Universitaet Bayreuth (Germany). $3 3545581
773 0 $t Dissertations Abstracts International $g 84-05B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29423294 $z click for full text (PQDT)