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Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application = = Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application =/
其他題名:	Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar.
作者:	Iskecman, Nilsu.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	91 p.
附註:	Source: Masters Abstracts International, Volume: 84-01.
Contained By:	Masters Abstracts International84-01.
標題:	Biocompatibility. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29154020
ISBN:	9798835547326

Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application = = Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar.
Iskecman, Nilsu.

Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application =Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 91 p.

Source: Masters Abstracts International, Volume: 84-01.

Thesis (M.Sc.)--Izmir Institute of Technology (Turkey), 2021.

This item must not be sold to any third party vendors.

Nowadays, modern functional wound dressings have become prominence due to their biocompatible, biodegradable, and non-toxic nature and ability to mimic ECM of skin. To enhance their mechanical and prolonged cumulative release of drugs in media, environmentally friendly inorganic nanofillers are preferred. Recently, halloysite nanotubes (HNTs) which is a new class of inorganic filler are using to improve mechanical properties and thanks to their tubular structure various agents encapsulated into these tubes to make drug release more controlled. In this thesis, it was aimed to fabricate and characterize bilayer wound dressing by using Zein-Vancomycin loaded HNTs nanofibers as upper layer to mimic ECM of skin and provide antibacterial protection and chitosan sponge as bottom layer to absorb the excess exudates of wound, provide gas transmission and facilitate the migration of inflammatory and fibroblast cells into the healing wounds. The morphology of nanofibers and encapsulation efficiency of HNTs are optimized to achieve similar homogeneous fiber structure with skin tissue and controlled release of Vancomycin drug. Chemical interaction between Vancomycin-HNT and Zein-HNT were characterized by FT-IR. The surface charge differences of HNTs by encapsulating drug into tubes was determined by zeta potential analysis. The weight loss percentage of nanofiber with the addition of HNTs into zein media was characterized by using thermogravimetric analysis (TGA). Bilayer sponges were characterized by SEM, FT-IR, porosity, mechanical properties, contact angle, water vapor transmission rate, swelling, degradation, cumulative drug release and their kinetics and antimicrobial activity analyzes. The diameter of drug loaded HNT-Zein nanofibers were found 202,7±0,05-225,2±0,06 nm which is in the range of native skin collagen fibril (50-500 nm) than can mimic ECM. The water vapor permeability of the wound dressings is between 2833 and 2490 g/m2day which is found in the appropriate range for wound healing. Bilayer dressings reached 78% cumulative release at the end of 14 days and the release medium showed antimicrobial activity against E. coli and for S. epidermidis. In conclusion, the developed drug-loaded bilayer mat has been found as a potential candidate for wound dressings applications to treat the chronic infections.

ISBN: 9798835547326Subjects--Topical Terms:

656157
Biocompatibility.

Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application = = Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar.
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245 1 0 $a Bilayer Chitosan/zein Based Nanofibers for Antimicrobial Wound Dressing Application = $b Antimikrobiyal yara ortusu uygulamalarina yonelik cift tabakali kitosan/zein temelli nanoelyaflar.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 91 p.
500 $a Source: Masters Abstracts International, Volume: 84-01.
500 $a Advisor: Tihminlioglu, Funda.
502 $a Thesis (M.Sc.)--Izmir Institute of Technology (Turkey), 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Nowadays, modern functional wound dressings have become prominence due to their biocompatible, biodegradable, and non-toxic nature and ability to mimic ECM of skin. To enhance their mechanical and prolonged cumulative release of drugs in media, environmentally friendly inorganic nanofillers are preferred. Recently, halloysite nanotubes (HNTs) which is a new class of inorganic filler are using to improve mechanical properties and thanks to their tubular structure various agents encapsulated into these tubes to make drug release more controlled. In this thesis, it was aimed to fabricate and characterize bilayer wound dressing by using Zein-Vancomycin loaded HNTs nanofibers as upper layer to mimic ECM of skin and provide antibacterial protection and chitosan sponge as bottom layer to absorb the excess exudates of wound, provide gas transmission and facilitate the migration of inflammatory and fibroblast cells into the healing wounds. The morphology of nanofibers and encapsulation efficiency of HNTs are optimized to achieve similar homogeneous fiber structure with skin tissue and controlled release of Vancomycin drug. Chemical interaction between Vancomycin-HNT and Zein-HNT were characterized by FT-IR. The surface charge differences of HNTs by encapsulating drug into tubes was determined by zeta potential analysis. The weight loss percentage of nanofiber with the addition of HNTs into zein media was characterized by using thermogravimetric analysis (TGA). Bilayer sponges were characterized by SEM, FT-IR, porosity, mechanical properties, contact angle, water vapor transmission rate, swelling, degradation, cumulative drug release and their kinetics and antimicrobial activity analyzes. The diameter of drug loaded HNT-Zein nanofibers were found 202,7±0,05-225,2±0,06 nm which is in the range of native skin collagen fibril (50-500 nm) than can mimic ECM. The water vapor permeability of the wound dressings is between 2833 and 2490 g/m2day which is found in the appropriate range for wound healing. Bilayer dressings reached 78% cumulative release at the end of 14 days and the release medium showed antimicrobial activity against E. coli and for S. epidermidis. In conclusion, the developed drug-loaded bilayer mat has been found as a potential candidate for wound dressings applications to treat the chronic infections.
520 $a Gunumuzde modern biyoaktif yara ortuleri, biyolojik olarak uyumlu, biyolojik olarak parcalanabilen, toksik olmayan ve cildin ekstraseluler matris yapisini taklit etme yetenekleri sayesinde on plana cikmislardir. Bu yara ortulerinin mekanik ozelliklerini iyilestirmek ve kumulatif ilac salim surelerini arttirmak icin cevre dostu inorganik nano-dolgular tercih edilir. Son zamanlarda, yeni bir inorganik dolgu sinifi olan halloysit nanotupler (HNT'ler) kullanilmaktadir. Karakteristik tubuler yapilari sayesinde ilac salinimini daha kontrollu hale getirmek icin cesitli ajanlar bu tuplere enkapsule edilebilmektedir. Bu tez calismasinda, cildin ECM'sini taklidi ve antibakteriyel koruma ozellikleri nedeniyle ust katman olarak Zein-Vancomycin yuklu HNTs nanofiberleri kullanilmistir. Ayrica alt tabaka olarak yaranin fazla eksudalarini absorbe etmesi, hava ve su buhari gecirgenligi saglamasi icin kitosan sunger kullanilmistir. Bu calismada iki katmandan olusan yara ortusunun uretilmesi ve karakterize edilmesi amaclanmistir. Nanoliflerin morfolojisi, HNT'lerin kapsulleme verimliligi ve kontrollu Vankomisin ilac salimi optimize edilmistir. Ust ve alt tabakalarin morfolojileri, taramali elektron mikroskobu (SEM) kullanilarak analiz edilmistir. Vankomisin-HNT ve Zein-HNT arasindaki kimyasal etkilesim, FT-IR ile karakterize edilmistir. Ilacin tuplere yuklenmesiyle olusan HNT'lerin yuzey yuk farkliliklari, zeta potansiyel analizi ile belirlenmistir. Zein polimer solusyonuna HNT'lerin eklenmesiyle degisen agirlik kaybi yuzdesi, termogravimetrik analiz (TGA) kullanilarak karakterize edilmistir. Nanolif tabakasinin hidrofilik karakterinin degisimi temas acisi olcumu ile hesaplanmistir. Cift katmanli sungerler SEM, FTIR, gozeneklilik, mekanik ozellikler, su buhari iletim hizi, sisme, bozunma, kumulatif ilac salimi, kinetik salim modelleri ve antimikrobiyal aktivite analizleri yapilmistir. Ilac yuklu HNT-Zein nanoliflerinin caplari 202,7±0,05-225,2±0,06 nm bulunmustur. Yara ortulerinin su buhari gecirgenligi 2833,3 ile 2490 /m2gun arasindadir ve gecirgenlik degerleri yara iyilesmesi icin uygun aralikta bulunmustur. Cift tabakali pansumanlar 14 gunun sonunda %78 kumulatif salim oranina ulasilmistir. Ayrica gram negatif (E. Coli) ve gram pozitif bakterilere (S. Epidermidis) karsi antimikrobiyal aktivite tespit edilmistir. Sonuc olarak, gelistirilen ilac yuklu cift katmanli yara ortusu, yara pansumanlari icin potansiyel aday olarak bulunmustur.
590 $a School code: 2215.
650 4 $a Biocompatibility. $3 656157
650 4 $a Physiology. $3 518431
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650 4 $a Nanoparticles. $3 605747
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650 4 $a Thermogravimetric analysis. $3 3681837
650 4 $a Bacteria. $3 550366
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650 4 $a Collagen. $3 892448
650 4 $a Permeability. $3 915594
650 4 $a Antibiotics. $3 670318
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650 4 $a Electric fields. $3 880423
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650 4 $a Developmental biology. $3 592588
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