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Effects of Cytotoxicity, Cellular Uptake, and Genotoxicity of Various Sizes and Concentrations of Chitosan Nanoparticles on Human Dental Pulp Cells.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Effects of Cytotoxicity, Cellular Uptake, and Genotoxicity of Various Sizes and Concentrations of Chitosan Nanoparticles on Human Dental Pulp Cells./
作者:	Alhomrany, Rami Mohammed.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	214 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-03, Section: B.
Contained By:	Dissertations Abstracts International83-03B.
標題:	Nanotechnology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28717701
ISBN:	9798538110650

Effects of Cytotoxicity, Cellular Uptake, and Genotoxicity of Various Sizes and Concentrations of Chitosan Nanoparticles on Human Dental Pulp Cells.
Alhomrany, Rami Mohammed.

Effects of Cytotoxicity, Cellular Uptake, and Genotoxicity of Various Sizes and Concentrations of Chitosan Nanoparticles on Human Dental Pulp Cells. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 214 p.

Source: Dissertations Abstracts International, Volume: 83-03, Section: B.

Thesis (D.Sc.D.)--Boston University, 2021.

This item must not be sold to any third party vendors.

This study evaluated the potential toxicity, genotoxicity, and cellular uptake of various sizes and concentrations of chitosan (CS) nanoparticles cultured with normal human dental pulp cells. Normal human dental pulp cells (hDPCs) were derived from human dental pulp tissues and cultured with (50-67) nm and (318-350) nm CS-nanoparticles in concentrations of 0.1 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL, and 4 mg/mL as study groups and 0 mg/mL as a control group for time intervals of 16 hours, 24 hours, 3 days, 7 days and 14 days. Attachment efficiency and proliferation rate were assessed by measuring the optical density of crystal violet-stained cells. Cell viability was determined by the activity of mitochondrial dehydrogenase enzymes. Genotoxicity was assessed using the cytokinesis-block micronucleus method and by measuring the fluorescent intensity of phosphorylated H2AX nuclear foci. Cellular uptake was determined by tagging chitosan nanoparticles with FITC stain and then measuring the fluorescence intensity of FITC-tagged chitosan nanoparticles using a spectrophotometer. Statistical analysis was performed using chi-square, one-way ANOVA, and post-hoc Tukey tests.All concentrations of the (50-67) nm group significantly reduced attachment efficiency in comparison with control (P< 0.01) and with (318-350) nm group (p<0.01). Proliferation rate and cell viability were significantly reduced in cells exposed to various concentrations of (50-67) nm chitosan when compared to (318-350) nm group (P<0.05) and control group (P<0.05). For both size groups, higher concentrations significantly showed lower proliferation rate and cell viability when compared to lower concentration (P< 0.01). CS-nanoparticles were able to internalize hDPCs and significantly induced micronuclei, nuclear buds, and pH2AX at concentrations of 0.5 mg/mL and 2 mg/mL as compared to 0.1 mg/mL (P<0.01) and control groups (P< 0.01). At both the 0.5 mg/mL and 2 mg/mL concentrations, (50-67) nm chitosan significantly induced higher proportions of micronuclei (P= 0.001), nuclear buds (P= 0.009), and pH2AX nuclear foci (P= 0.00004) compared to (318-350) nm chitosan. In conclusion, CS-nanoparticles at sizes (50-67) nm and (318-350) nm at a concentration of (0.5-4) mg/mL internalized hDPCs and exhibited cytotoxic and genotoxic effects in dose-dependent and size-associated manners.

ISBN: 9798538110650Subjects--Topical Terms:

526235
Nanotechnology.
Subjects--Index Terms:

Chitosan

Effects of Cytotoxicity, Cellular Uptake, and Genotoxicity of Various Sizes and Concentrations of Chitosan Nanoparticles on Human Dental Pulp Cells.
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