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Improving Viral Pathogen Discovery, Characterization, and Surveillance through High-Throughput Antibody Screening Methods.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Improving Viral Pathogen Discovery, Characterization, and Surveillance through High-Throughput Antibody Screening Methods./
作者:	Heffron, Anna Siobhan.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	296 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-01, Section: B.
Contained By:	Dissertations Abstracts International83-01B.
標題:	Virology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28547501
ISBN:	9798516930799

Improving Viral Pathogen Discovery, Characterization, and Surveillance through High-Throughput Antibody Screening Methods.
Heffron, Anna Siobhan.

Improving Viral Pathogen Discovery, Characterization, and Surveillance through High-Throughput Antibody Screening Methods. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 296 p.

Source: Dissertations Abstracts International, Volume: 83-01, Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2021.

This item must not be sold to any third party vendors.

Six Public Health Emergencies of International Concern have been declared since the establishment of the World Health Organization (WHO)'s Emergency Committee in 2005. These emergencies were all viral diseases: pandemic H1N1 influenza in 2009, poliovirus resurgence in 2014, the outbreak of Ebola in West Africa in 2014, the emergence of Zika virus in the Americas in 2016, another Ebola outbreak in central Africa in 2018, and most recently the emergency of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing the coronavirus disease of 2019 (COVID-19) pandemic. With the exception of poliovirus, most of these are zoonotic, vector-borne, or both. Without exception, the human response to these emergences has been reactive, perpetually caught by surprise by these outbreaks and often forced to start from a place of catching up with the developments of these new or newly-emerging viral pathogens. The increasing frequency of these emergences demonstrates the need for ready pathways for rapid development of diagnostic, preventive, and treatment modalities, and they further demonstrate the need for better viral surveillance and virus discovery systems, so that we might not be caught quite so unaware with the next viral emergence. Efforts at an efficient, effective response to each recent viral emergence has been hindered by a lack of rapid, accessible and accurate diagnostic testing and prepared, easily adaptable medical countermeasures.In this thesis, I combine a variety of microbiology techniques-including microscopy, use of animal models, polymerase chain reaction, enzyme-linked immunosorbent assays, and neutralization assays-alongside the emerging and evolving technologies of NGS and ultradense peptide array analysis to probe the limits of our understanding of rapid viral discovery and characterization. By combining these technologies to study novel viruses and viruses emerging to cause new disease, my work aims to provide a window into how future emerging infectious threats may be more effectively detected and characterized. In chapter 2, we use next generation sequencing and novel virus discovery computational methods to investigate a potential infectious cause for an increased rate of lymphocytic enterocolitis in common marmosets at the Wisconsin National Primate Research Center. In chapter 3, we use a macaque model to test and develop a novel ultradense peptide microarray to discern the cross-reactivity and the unique aspects of close-related flaviviruses. In chapter 4, using similar technology and bioinformatic pipelines to those in chapter 3, we are able to apply the technology developed in chapter 3 to the emerging viral threat SARS-CoV-2. Building upon the work presented here will increase our understanding of and capacity to detect and combat current and future emerging pathogens.

ISBN: 9798516930799Subjects--Topical Terms:

642304
Virology.
Subjects--Index Terms:

Antibody

Improving Viral Pathogen Discovery, Characterization, and Surveillance through High-Throughput Antibody Screening Methods.
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