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Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease = = INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease =/
其他題名:	INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu.
作者:	Terlemez, Gamze.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	118 p.
附註:	Source: Masters Abstracts International, Volume: 83-11.
Contained By:	Masters Abstracts International83-11.
標題:	Pathogens. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29079296
ISBN:	9798426877061

Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease = = INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu.
Terlemez, Gamze.

Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease =INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 118 p.

Source: Masters Abstracts International, Volume: 83-11.

Thesis (Master's)--Izmir Institute of Technology (Turkey), 2021.

This item must not be sold to any third party vendors.

Inflammatory bowel disease (IBD) is complex, multifactorial, polygenic diseases without a certain cure. Patients require lifelong surveillance, expensive treatments, risky surgeries, have increased cancer and other systemic disease risks. IBD consists of two groups as Ulcerative colitis (UC) and Crohn's disease (CD). Although pathogenesis of IBD is not clear, a dysregulated immune response to environmental factors (intestinal microbiota) develops in genetically susceptible individuals. As early diagnosis is hard, most patients are diagnosed at a later stage, however, they suffer from colon damage and lose the chance of early treatment.This thesis aimed to identify biomarkers that can analyze IBD patient risk profiles to control disease and reveal individuals at risk before the disease progresses further. Firstly, mutations in Turkish IBD patients were investigated in candidate regions of innate immunity (NOD2), adaptive immunity (IL-23R), and autophagy (ATG16L1) genes. Then, microbiota profiles of patients were revealed by NGS and qPCR. Finally, to identify relationship between mutations and microbiota, statistical analyses were performed with non-parametric methods. As a result of this study, Shannon index showed significant reduction of bacterial diversity in IBD patients versus the control group (p-value, 0.048). At the species level, relative abundance of F. prausnitzii decreased while abundance of E. coli increased in IBD patients. Significant relationship (p-value, 0.034) was found between rs11209026 genotype and F. prausnitzii reduction in CD but not in UC. In conclusion, converting significant observations into a molecular test kit can provide a molecular guide that helps gastroenterologists in planning personalized treatments in IBD patients.

ISBN: 9798426877061Subjects--Topical Terms:

3540520
Pathogens.

Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease = = INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu.
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245 1 0 $a Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease = $b INflamatuvar Bagirsak Hastaligi'nda Genetik ve Mikrobiyal Degisikliklerin Korelasyonu.
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300 $a 118 p.
500 $a Source: Masters Abstracts International, Volume: 83-11.
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506 $a This item must not be sold to any third party vendors.
520 $a Inflammatory bowel disease (IBD) is complex, multifactorial, polygenic diseases without a certain cure. Patients require lifelong surveillance, expensive treatments, risky surgeries, have increased cancer and other systemic disease risks. IBD consists of two groups as Ulcerative colitis (UC) and Crohn's disease (CD). Although pathogenesis of IBD is not clear, a dysregulated immune response to environmental factors (intestinal microbiota) develops in genetically susceptible individuals. As early diagnosis is hard, most patients are diagnosed at a later stage, however, they suffer from colon damage and lose the chance of early treatment.This thesis aimed to identify biomarkers that can analyze IBD patient risk profiles to control disease and reveal individuals at risk before the disease progresses further. Firstly, mutations in Turkish IBD patients were investigated in candidate regions of innate immunity (NOD2), adaptive immunity (IL-23R), and autophagy (ATG16L1) genes. Then, microbiota profiles of patients were revealed by NGS and qPCR. Finally, to identify relationship between mutations and microbiota, statistical analyses were performed with non-parametric methods. As a result of this study, Shannon index showed significant reduction of bacterial diversity in IBD patients versus the control group (p-value, 0.048). At the species level, relative abundance of F. prausnitzii decreased while abundance of E. coli increased in IBD patients. Significant relationship (p-value, 0.034) was found between rs11209026 genotype and F. prausnitzii reduction in CD but not in UC. In conclusion, converting significant observations into a molecular test kit can provide a molecular guide that helps gastroenterologists in planning personalized treatments in IBD patients.
520 $a Inflamatuvar Bagirsak Hastaligi (IBH) gunumuzun sik rastlanan, kesin bir tedavisi olmayan, etkiledigi kisilerde hayat boyu takibi, pahali tedavileri ve riskli ameliyatlari gerektiren, kanser riski ve diger sistematik rahatsizliklari artiran, ciddi is gucu ve yasam kalitesi kayiplarina yol acan karmasik, multifaktoriyel, multigenik bir hastaliktir. IBH temel olarak Ulseratif kolit (UK) ve Crohn hastaligi (CH) olarak iki grupta incelenir. Hastaligin patogenezi net olarak bilinmemekle birlikte, genetik olarak yatkin bireylerde, cevresel faktorlere (intestinal mikrobiyota) karsi disregule immun yanit olusmaktadir. Erken tanisinda zorluklar yasandigi icin hastalar, hastaligin ilerlemis safhalarinda bir uzman tarafindan teshis bulmakta, zaman kaybindan dolayi bagirsak dokusunda zararlar artmakta, erken tedavi sansi kaybolmaktadir.Bu tezde, hastaligi kontrol altina almak ve hastalik daha fazla ilerlemeden risk altindaki bireylerin ortaya cikarilmasi icin IBH hasta risk profilini analiz edecek biyobelirteclerin belirlenmesi amaclanmistir. Ilk olarak dogal immunite (NOD2), adaptif immunite (IL-23R) ve otofaji (ATG16L1) genlerinin aday bolgelerinde Turk IBD hastalarina ozgu mutasyonlar arastirildi. Ardindan hastalarin mikrobiyota profilleri, amplikon dizileme ve qPCR ile ortaya cikarildi. Son olarak, Turk IBD hastalarinda, mutasyonlar ve mikrobiyota arasindaki iliskiyi belirlemek icin parametrik olmayan yontemlerle istatistiksel analizler yapildi. Calisma sonucunda, Shannon indeksi, kontrol grubuna kiyasla IBD hastalarinda bakteri cesitliliginde onemli bir azalma oldugunu gostermistir (p-degeri, 0.048). IBD hastalarinda tur duzeyinde F. prausnitzii bakterisinin goreceli bollugu azalirken E. coli bakterisinin artti. UK'in aksine, rs11209026 genotipi ile CH' deki F. prausnitzii azalmasi arasinda anlamli iliski (p-degeri, 0.034) bulundu. Sonuc olarak, tum bu gozlemlerin molekuler bir test kitine donusturulmesi, gastroenterologlarin IBH hastalarinda kisisellestirilmis tedaviler planlamasina yardimci olacak bir altyapi saglayabilir.
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