東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus./
作者:	Ramos, Brigette.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:	39 p.
附註:	Source: Masters Abstracts International, Volume: 82-02.
Contained By:	Masters Abstracts International82-02.
標題:	Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27957313
ISBN:	9798662441613

The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus.
Ramos, Brigette.

The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 39 p.

Source: Masters Abstracts International, Volume: 82-02.

Thesis (M.S.)--Rutgers The State University of New Jersey, School of Graduate Studies, 2020.

This item must not be sold to any third party vendors.

Fetal Alcohol Syndrome (FAS) is a condition, particularly found in children, characterized by damage to the central nervous system as a result of alcohol exposure during a woman's pregnancy (Wilhoit et al., 2017). Areas of the brain that are affected include the hippocampus, basal ganglia, cerebellum, and hypothalamus. Once exposed to alcohol, the production of Beta-endorphins (βEP), a neuronal peptide involved in inhibiting stress in the hypothalamic arcuate nucleus, is reduced (Sprouse-Blum et al., 2010). On the other hand, there are cells, such as microglia, that can be stimulated during alcohol exposure. Microglia perform several essential functions in the brain, such as a role in neuroinflammation, regulation of brain development, and work as CNS macrophages (Colonna et al., 2017). Microglia are also capable of releasing exosomes, small membrane vesicles involved in neuronal communication and immune functions, including adaptive and innate immune responses (Fruhbeis et al., 2012).Using a rat animal model, our overall objective revolved around how exosomes, derived from genetically modified microglial cells, could be introduced through the periphery to influence βEP and glial interaction in the brain, and whether transportation of these exosomes could be altered by alcohol administration. We observed that the exosomes successfully passed through the blood-brain barrier (BBB) and were able to deposit into the hypothalamic arcuate nucleus. There was an increase in exosome deposition and a reduction in βEP neurons in alcohol treated rats, in comparison to the control or non-treated animal models. However, the exosomes displayed an inability to colocalize within the βEP neurons in both alcohol and non-treated animals. Therefore, we found that genetically modified exosomes can bypass the BBB and travel into the brain to localize into the hypothalamic arcuate area, more so through alcohol administration, and affect glial and βEP communication. Further research in observing where the exosomes are colocalizing is essential in finding what other roles the exosomes are involved in.

ISBN: 9798662441613Subjects--Topical Terms:

518431
Physiology.
Subjects--Index Terms:

Fetal Alcohol Syndrome

The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus.
LDR:03281nmm a2200361 4500 001 2347039
005 20220711065231.5
008 241004s2020 ||||||||||||||||| ||eng d
020 $a 9798662441613
035 $a (MiAaPQ)AAI27957313
035 $a AAI27957313
040 $a MiAaPQ $c MiAaPQ
100 1 $a Ramos, Brigette. $3 3686248
245 1 4 $a The Effect of Alcohol on Genetically Modified Exosome Deposition in the Hypothalamus.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 39 p.
500 $a Source: Masters Abstracts International, Volume: 82-02.
500 $a Advisor: Sarkar, Dipak;Fan, Huizhou.
502 $a Thesis (M.S.)--Rutgers The State University of New Jersey, School of Graduate Studies, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Fetal Alcohol Syndrome (FAS) is a condition, particularly found in children, characterized by damage to the central nervous system as a result of alcohol exposure during a woman's pregnancy (Wilhoit et al., 2017). Areas of the brain that are affected include the hippocampus, basal ganglia, cerebellum, and hypothalamus. Once exposed to alcohol, the production of Beta-endorphins (βEP), a neuronal peptide involved in inhibiting stress in the hypothalamic arcuate nucleus, is reduced (Sprouse-Blum et al., 2010). On the other hand, there are cells, such as microglia, that can be stimulated during alcohol exposure. Microglia perform several essential functions in the brain, such as a role in neuroinflammation, regulation of brain development, and work as CNS macrophages (Colonna et al., 2017). Microglia are also capable of releasing exosomes, small membrane vesicles involved in neuronal communication and immune functions, including adaptive and innate immune responses (Fruhbeis et al., 2012).Using a rat animal model, our overall objective revolved around how exosomes, derived from genetically modified microglial cells, could be introduced through the periphery to influence βEP and glial interaction in the brain, and whether transportation of these exosomes could be altered by alcohol administration. We observed that the exosomes successfully passed through the blood-brain barrier (BBB) and were able to deposit into the hypothalamic arcuate nucleus. There was an increase in exosome deposition and a reduction in βEP neurons in alcohol treated rats, in comparison to the control or non-treated animal models. However, the exosomes displayed an inability to colocalize within the βEP neurons in both alcohol and non-treated animals. Therefore, we found that genetically modified exosomes can bypass the BBB and travel into the brain to localize into the hypothalamic arcuate area, more so through alcohol administration, and affect glial and βEP communication. Further research in observing where the exosomes are colocalizing is essential in finding what other roles the exosomes are involved in.
590 $a School code: 0190.
650 4 $a Physiology. $3 518431
650 4 $a Toxicology. $3 556884
650 4 $a Neurosciences. $3 588700
653 $a Fetal Alcohol Syndrome
653 $a Beta-endorphins
653 $a Microglia
653 $a Hypothalamus
690 $a 0719
690 $a 0383
690 $a 0317
710 2 $a Rutgers The State University of New Jersey, School of Graduate Studies. $b Physiology and Integrative Biology. $3 3686249
773 0 $t Masters Abstracts International $g 82-02.
790 $a 0190
791 $a M.S.
792 $a 2020
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27957313