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Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli./
作者:
Gargatte, Sweta.
出版者:
Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:
39 p.
附註:
Source: Masters Abstracts International, Volume: 83-02.
Contained By:
Masters Abstracts International83-02.
標題:
Chemical engineering. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28322846
ISBN:
9798534660159
Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
Gargatte, Sweta.
Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
- Ann Arbor : ProQuest Dissertations & Theses, 2021 - 39 p.
Source: Masters Abstracts International, Volume: 83-02.
Thesis (M.S.)--Rutgers The State University of New Jersey, School of Graduate Studies, 2021.
This item must not be sold to any third party vendors.
4-hydroxystyrene is a phenolic chemical compound used in polymer and flavor industries. Microbial biosynthesis of this compound has been studied over recent years as a sustainable green alternative to synthetic chemical production. In this current study, 4-hydroxystyrene was biosynthesized using engineered E. coli. To improve the hydroxystyrene biosynthesis utilizing the carbon source following strategies were adopted. The pathway for bioproduction was bifurcated, and the co-culture of E. coli was implemented over monoculture. An amino acid exporter protein PhpCAT was utilized in combination biosensor-assisted cell selection mechanism to maximize the pathway intermediate tyrosine's biosynthesis. The exporter improved tyrosine's mass transfer between the co-culture members, and the resulting co-culture produced 298 mg/L 4-hydroxystyrene from 5 g/L glucose, which is 96% higher than the control co-culture without the exporter. The achieved yield of 0.06 g/g glucose is the highest among previous studies on de novo microbial biosynthesis. It shows that the exporter protein's utilization and biosensors enhance the pathway intermediate in a co-culture study and boost the hydroxystyrene biosynthesis performance. This strategy can pave the path for microbial biosynthesis for all the potential aromatic-derived compounds.
ISBN: 9798534660159Subjects--Topical Terms:
560457
Chemical engineering.
Subjects--Index Terms:
Amino acid exporter
Engineering for 4-Hydroxystyrene Biosynthesis by E. Coli.
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4-hydroxystyrene is a phenolic chemical compound used in polymer and flavor industries. Microbial biosynthesis of this compound has been studied over recent years as a sustainable green alternative to synthetic chemical production. In this current study, 4-hydroxystyrene was biosynthesized using engineered E. coli. To improve the hydroxystyrene biosynthesis utilizing the carbon source following strategies were adopted. The pathway for bioproduction was bifurcated, and the co-culture of E. coli was implemented over monoculture. An amino acid exporter protein PhpCAT was utilized in combination biosensor-assisted cell selection mechanism to maximize the pathway intermediate tyrosine's biosynthesis. The exporter improved tyrosine's mass transfer between the co-culture members, and the resulting co-culture produced 298 mg/L 4-hydroxystyrene from 5 g/L glucose, which is 96% higher than the control co-culture without the exporter. The achieved yield of 0.06 g/g glucose is the highest among previous studies on de novo microbial biosynthesis. It shows that the exporter protein's utilization and biosensors enhance the pathway intermediate in a co-culture study and boost the hydroxystyrene biosynthesis performance. This strategy can pave the path for microbial biosynthesis for all the potential aromatic-derived compounds.
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http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28322846
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