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Characterization and Control of the Material Properties in DLP 3D Printed Parts for Use in Tissue Engineering and Regeneration.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization and Control of the Material Properties in DLP 3D Printed Parts for Use in Tissue Engineering and Regeneration./
作者:	Uzcategui, Asais Camila.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	141 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-03, Section: B.
Contained By:	Dissertations Abstracts International83-03B.
標題:	Mechanical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28544230
ISBN:	9798538119134

Characterization and Control of the Material Properties in DLP 3D Printed Parts for Use in Tissue Engineering and Regeneration.
Uzcategui, Asais Camila.

Characterization and Control of the Material Properties in DLP 3D Printed Parts for Use in Tissue Engineering and Regeneration. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 141 p.

Source: Dissertations Abstracts International, Volume: 83-03, Section: B.

Thesis (Ph.D.)--University of Colorado at Boulder, 2021.

This item must not be sold to any third party vendors.

A predominant challenge in tissue engineering is the need for a robust technique for producing structures with precise three-dimensional control of mechanical properties. This is in part because stem cell differentiation is sensitive to biomechanical cues and scaffolds must be able to withstand the mechanical forces in vivo. 3D printing hydrogels via digital light projection (DLP) lithography is a promising method for creating mechanically designed regenerative medical implants. However, the mechanical and chemical properties of materials patterned using light-based 3D printing techniques are largely unpredictable and often undesirable. Furthermore, the 3D printing process parameters play a pivotal role in the properties of the final part, which begs the question: Can these be used to control the crosslink density and mechanical properties at the micro and macro scale?The goal of this dissertation was to provide a fundamental understanding of the DLP 3D printing process and use that knowledge to spatially control the material properties of 3D printed parts for eventual use in biomedical applications. Initial work focused on using polymerization kinetics to improve the understanding of resin behavior during DLP 3D printing. An improved working curve model was developed to account for the resin's sublinear response to irradiation intensity, giving a more accurate method to determine cure depth and determining z-resolution. The understanding gained by this model was then used to implement a dual-cure material design that reduced the staircase effect, achieved micro-scale vertical feature precision, and enhanced the mechanical properties at the macro-scale. These experiments demonstrated microscale control of geometry and the fabrication of parts indistinguishable from uniformly bulk-cured parts. A scaled exposure relationship was used to explore microscale mechanical control to direct inter and intra-layer gradient profiles. Smooth gradients of 30 MPa over ≈75 µm and sharp step-changes of 30 MPa over ≈5 µm (25x steeper than the cure depth) were achieved in acrylate-based 3D constructs tested by atomic force microscopy. Transport as an additional knob for macro- and micro-scale property control was explored. Changes in 3D printing wait time impacted the interlayer and intralayer modulus gradients. In collaborations across the University of Colorado, mechanically enhanced 3D printed scaffolds were used for in vivo and in vitro tissue engineering studies. This dissertation demonstrated that a fundamental understanding of the DLP 3D printing process extends the scope of 3D printing by fabricating parts with high spatial resolution (1-10's microns), functional mechanical gradients, and enhanced interfacial properties. With this fundamental understanding, demonstration of mechanical control in 3D printed parts, and application to tissue engineering, this work provides a basis for creating biomimetic 3D tissue scaffolds.

ISBN: 9798538119134Subjects--Topical Terms:

649730
Mechanical engineering.
Subjects--Index Terms:

3D printing

Characterization and Control of the Material Properties in DLP 3D Printed Parts for Use in Tissue Engineering and Regeneration.
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