東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Linked to FindBook

Google Book

Amazon

博客來

Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell./
Author:	Oliveira, Ryan David.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	79 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
Subject:	Veterinary services. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28644507
ISBN:	9798471133617

Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell.
Oliveira, Ryan David.

Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 79 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (Ph.D.)--Washington State University, 2021.

This item must not be sold to any third party vendors.

Coxiella burnetii is a bacterial pathogen distributed around the globe that causes disease in both animals and humans. Humans can develop a syndrome called "Q fever", characterized by a flulike syndrome or pneumonia and hepatitis in the acute phase and by potentially fatal endocarditis or vascular infection in some chronic cases. Sheep and goats infected with C. burnetii can suffer clusters of fetal loss that, via aerosol from birth fluids and the placenta, are a major source of infection for humans. The infectious dose in humans is as low as one bacterium, and the organism is hardy in the environment. On a cellular level, C. burnetii preferentially infects macrophages, a cell type derived in many cases from circulating monocytes, and macrophages are considered important in both the survival and clearance of C. burnetii infection. To better understand and control this transmission cycle, we sought to characterize the infection in sheep both by observing shedding in the natural setting and by conducting a genome-wide association with absolute monocyte counts in sheep blood. In chapter one, we measure prevalence to antibodies against C. burnetii (seroprevalence) and shedding by multiple routes on a large sheep farm that had been associated with a human transmission event of Q fever three decades prior. Our findings demonstrate no detectable levels of C. burnetii by polymerase chain reaction and a seroprevalence similar to that of the average large sheep farm in the United States, challenging a previously held assumption that infection persists indefinitely once established on a premises. Our discussion speculates on reasons shedding may have ceased in the absence of targeted intervention and considers the implications of this finding. In chapter two, we perform a genome-wide association with absolute counts of circulating monocytes in sheep. Several significant sites are discovered, and the regions for which they are predictive includes genes involved in hematopoiesis, macrophage activation, and monocyte differentiation. The results have implications for genetic influences on an important effector cell in C. burnetii infection. Together, these data shed light on both the natural transmission cycle and possible immunologic genes involved in infection.

ISBN: 9798471133617Subjects--Topical Terms:

3433982
Veterinary services.
Subjects--Index Terms:

Coxiella burnetii

Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell.
LDR:03453nmm a2200361 4500 001 2342382
005 20220318093129.5
008 241004s2021 ||||||||||||||||| ||eng d
020 $a 9798471133617
035 $a (MiAaPQ)AAI28644507
035 $a AAI28644507
040 $a MiAaPQ $c MiAaPQ
100 1 $a Oliveira, Ryan David. $3 3680734
245 1 0 $a Characterizing the Duration of Coxiella Burnetii Shedding and the Genetic Influence Behind a Key Immune Effector Cell.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 79 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
500 $a Advisor: White, Stephen;Neibergs, Holly.
502 $a Thesis (Ph.D.)--Washington State University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Coxiella burnetii is a bacterial pathogen distributed around the globe that causes disease in both animals and humans. Humans can develop a syndrome called "Q fever", characterized by a flulike syndrome or pneumonia and hepatitis in the acute phase and by potentially fatal endocarditis or vascular infection in some chronic cases. Sheep and goats infected with C. burnetii can suffer clusters of fetal loss that, via aerosol from birth fluids and the placenta, are a major source of infection for humans. The infectious dose in humans is as low as one bacterium, and the organism is hardy in the environment. On a cellular level, C. burnetii preferentially infects macrophages, a cell type derived in many cases from circulating monocytes, and macrophages are considered important in both the survival and clearance of C. burnetii infection. To better understand and control this transmission cycle, we sought to characterize the infection in sheep both by observing shedding in the natural setting and by conducting a genome-wide association with absolute monocyte counts in sheep blood. In chapter one, we measure prevalence to antibodies against C. burnetii (seroprevalence) and shedding by multiple routes on a large sheep farm that had been associated with a human transmission event of Q fever three decades prior. Our findings demonstrate no detectable levels of C. burnetii by polymerase chain reaction and a seroprevalence similar to that of the average large sheep farm in the United States, challenging a previously held assumption that infection persists indefinitely once established on a premises. Our discussion speculates on reasons shedding may have ceased in the absence of targeted intervention and considers the implications of this finding. In chapter two, we perform a genome-wide association with absolute counts of circulating monocytes in sheep. Several significant sites are discovered, and the regions for which they are predictive includes genes involved in hematopoiesis, macrophage activation, and monocyte differentiation. The results have implications for genetic influences on an important effector cell in C. burnetii infection. Together, these data shed light on both the natural transmission cycle and possible immunologic genes involved in infection.
590 $a School code: 0251.
650 4 $a Veterinary services. $3 3433982
650 4 $a Animal diseases. $3 3181862
650 4 $a Pathology. $3 643180
653 $a Coxiella burnetii
653 $a Monocytes
653 $a Q fever
653 $a Sheep
690 $a 0778
690 $a 0476
690 $a 0571
710 2 $a Washington State University. $b College of Veterinary Medicine. $3 3680669
773 0 $t Dissertations Abstracts International $g 83-05B.
790 $a 0251
791 $a Ph.D.
792 $a 2021
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28644507