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Coordinated Transcriptional Regulati...

Duduit, James Robert.

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Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars./
作者:	Duduit, James Robert.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:	99 p.
附註:	Source: Masters Abstracts International, Volume: 82-07.
Contained By:	Masters Abstracts International82-07.
標題:	Plant sciences. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28122830
ISBN:	9798664736564

Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars.
Duduit, James Robert.

Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 99 p.

Source: Masters Abstracts International, Volume: 82-07.

Thesis (M.Sc.)--North Carolina State University, 2020.

This item must not be sold to any third party vendors.

Lycopene content in tomato fruit is largely under genetic control and varies greatly among genotypes. Little is known about the molecular mechanisms regulating fruit lycopene content in high-lycopene tomatoes. In the present study, 42 potential high-lycopene tomato cultigens with different genetic backgrounds were collected worldwide. High performance liquid chromatography (HPLC) analysis was used to quantify fruit carotenoid (lycopene, phytofluene, prolycopene, and beta-carotene) contents at four fruit developmental stages (i.e., breaker, orange, pink, and ripe) of each cultigen. Real-time RT-PCR was used to quantify the relative expression levels of all the 25 pathway genes individually at the breaker and ripe stages. In these cultigens, we found i) a general trend of strong expression of upstream genes prior to lycopene biosynthesis and weak expression of most downstream genes at both stages; ii) significant higher expression in 7 upstream genes and 8 downstream genes at the breaker or both stages than in the negative control cultigen Moneymaker; and iii) significant higher phytofluene, lycopene and betacarotene contents during fruit ripening than in Moneymaker. Thus, coordinated transcriptional regulation of the pathway genes contributed to significantly higher metabolic flux flow into the pathway in these cultigens than in Moneymaker, leading to higher fruit lycopene content. This was the first systematic investigation of the role of the complete carotenoid biosynthesis pathway genes in fruit lycopene content across many high-lycopene cultigens, which will enable tomato breeding and gene editing for improved fruit lycopene content.

ISBN: 9798664736564Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

High-lycopene cultigens

Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars.
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