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Dawn of a New Era in Sponge Biotechn...

van Deinsen‐Hesp, Kylie .

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Dawn of a New Era in Sponge Biotechnology.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Dawn of a New Era in Sponge Biotechnology./
作者:	van Deinsen‐Hesp, Kylie .
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	169 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-02, Section: B.
Contained By:	Dissertations Abstracts International83-02B.
標題:	Water shortages. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28591326
ISBN:	9798522949419

Dawn of a New Era in Sponge Biotechnology.
van Deinsen‐Hesp, Kylie .

Dawn of a New Era in Sponge Biotechnology. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 169 p.

Source: Dissertations Abstracts International, Volume: 83-02, Section: B.

Thesis (Ph.D.)--Wageningen University and Research, 2021.

This item must not be sold to any third party vendors.

Sponges are ancient filter‐feeding animals that seem to have barely changed since they evolved over 600 million years ago. Water enters through pores connecting to a myriad of channels, pumped through chambers where choanocytes take up particles or dissolved nutrients, and then expelled through oscula. However, this seemingly simple system conceals a complex conglomerate of chemical compounds, many of which are biologically active. These chemicals allow sponges to interact with their surroundings and protect themselves. For example, antibacterial and antiviral compounds ward off potential pathogens, while cytostatic compounds prevent other competing organisms from growing. When such compounds were first discovered, sponges were quickly recognized as a treasure trove of potential new drugs. However, a bottleneck emerged that would stymie sponge cell culture for decades: obtaining enough biomass to produce compounds at a large enough scale for clinical trials. Many studiesfocused on developing sponge cell lines, which would provide a scalable platform to produce compounds in a controlled environment. Despite all efforts and promising results, no sponge cell lines were established. In Chapter 2 we reported a long‐awaited breakthrough: cells of 9 sponge species divided rapidly in amino acid‐optimized nutrient medium M1, based on mammalian cell culture medium 199 (M199). The fastest dividing cells doubled in under an hour. Cells of speciesthat responded to M1 medium could also divide in the more basal marine‐adjusted M199, albeit usually slower and to a lower density. Among these species were 3 members of the genus Geodia, G. neptuni, Geodia sp., and G. barretti, which showed most consistent results between different individuals. The Geodia spp. were subcultured 3 ‐ 5 times over a period of 21 ‐ 35 days and reached an average total of 6 population doublings. All 3 species were able to proliferate at both 4 °C, the temperature used for G. barretti, and 22 °C, at which G. neptuni and Geodia sp. were incubated. The finite cell lines we developed represented the first real leads to develop stable or continuous marine sponge cell lines.We followed up on this lead for G. barretti in Chapter 3 and established the first continuous marine sponge cell line. Cells of 3 individuals G. barretti were cultured in OpM1 medium, the successor of M1 with added growth factors, lipids, vitamins, and other nutrients. In OpM1, G. barretti cells proliferated even more rapidly and to a higher density than in M1 medium. We analyzed the impact of the individual components in OpM1 and determined that phytohemagglutinin (PHA) was a critical component. Besides this finding, all‐but‐ one of the added nutrient mixtures in OpM1 contributed to the differences in cell division between OpM1 and M1. In sub culturing experiments, G. barretti cells of 3 individuals could double nearly 100 times, compared to 5 doublings cells of the same individuals reached in M1. The maximum number of doublings of the G. barretti cell line has yet to be determined. Subcultured cells could be cryopreserved and thawed to inoculate new cultures. These results brought us one step closer to sponge cells producing biopharmaceuticals at industrial scale.

ISBN: 9798522949419Subjects--Topical Terms:

3560043
Water shortages.

Dawn of a New Era in Sponge Biotechnology.
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