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Studies on the Structure, Properties...

Scott, Liam.

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Studies on the Structure, Properties, and Potential Applications of Virus-Like Particles for Biomedical Applications.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Studies on the Structure, Properties, and Potential Applications of Virus-Like Particles for Biomedical Applications./
Author:	Scott, Liam.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	56 p.
Notes:	Source: Masters Abstracts International, Volume: 82-12.
Contained By:	Masters Abstracts International82-12.
Subject:	Biomechanics. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28493217
ISBN:	9798505545980

Studies on the Structure, Properties, and Potential Applications of Virus-Like Particles for Biomedical Applications.
Scott, Liam.

Studies on the Structure, Properties, and Potential Applications of Virus-Like Particles for Biomedical Applications. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 56 p.

Source: Masters Abstracts International, Volume: 82-12.

Thesis (M.S.)--Indiana University, 2021.

This item must not be sold to any third party vendors.

Viruses are infectious biological particles that cause illness and death across all clades of life. However, some viruses have been identified as helpful agents for a variety of biomedical applications. As such, studies on virus particles are doubly important, as they provide insight into how to stop viral infections, and into how to design better virus-based biomedical tools. In the first section of this work, we describe studies focused on better understanding how the ssRNA virus, Brome mosaic virus (BMV), responds to high levels of osmotic pressure that are encountered in biological fluids and cell interiors. We also study the influence of genetic cargo on the BMV capsid, and find that genetic cargo can act to pre-stress and even reinforce BMV capsids. In the second section of this work, we describe experiments where we labeled BMV with high densities of the fluorescent dye Oregon Green-488 (OG-488), and then excited these labeled particles with ultrafast laser pulses. We did this in an attempt to overcome the phenomenon of concentration quenching, which significantly limits the brightness of labeled structures in fluorescence guided surgery (FGS). From these experiments, we found that BMV particles labeled with greater than 180 dye molecules display a recovery from concentration quenching. Therefore, we decided to build upon this phenomenon by moving to a larger virus, MPyV, in order to label a virus particle with an even greater numbers of dyes. We found that MPyV could be labeled with a greater number of OG-488 dyes than BMV, however the particles were heterogeneous in their labeling. Therefore, in the third section of this work, we describe the design, purification, and assembly of a fusion between the capsid protein of MPyV and super folder green fluorescent protein (sfGFP). This provides proof-of-principle that it is possible to assemble a virus like particle that is homogeneously labeled with exactly one fluorophore per structural protein. Overall, the studies detailed in this text provide insight into the mechanical properties of viruses, as well as how those properties might be utilized for biomedical applications.

ISBN: 9798505545980Subjects--Topical Terms:

548685
Biomechanics.
Subjects--Index Terms:

Brome mosaic virus

Studies on the Structure, Properties, and Potential Applications of Virus-Like Particles for Biomedical Applications.
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520 $a Viruses are infectious biological particles that cause illness and death across all clades of life. However, some viruses have been identified as helpful agents for a variety of biomedical applications. As such, studies on virus particles are doubly important, as they provide insight into how to stop viral infections, and into how to design better virus-based biomedical tools. In the first section of this work, we describe studies focused on better understanding how the ssRNA virus, Brome mosaic virus (BMV), responds to high levels of osmotic pressure that are encountered in biological fluids and cell interiors. We also study the influence of genetic cargo on the BMV capsid, and find that genetic cargo can act to pre-stress and even reinforce BMV capsids. In the second section of this work, we describe experiments where we labeled BMV with high densities of the fluorescent dye Oregon Green-488 (OG-488), and then excited these labeled particles with ultrafast laser pulses. We did this in an attempt to overcome the phenomenon of concentration quenching, which significantly limits the brightness of labeled structures in fluorescence guided surgery (FGS). From these experiments, we found that BMV particles labeled with greater than 180 dye molecules display a recovery from concentration quenching. Therefore, we decided to build upon this phenomenon by moving to a larger virus, MPyV, in order to label a virus particle with an even greater numbers of dyes. We found that MPyV could be labeled with a greater number of OG-488 dyes than BMV, however the particles were heterogeneous in their labeling. Therefore, in the third section of this work, we describe the design, purification, and assembly of a fusion between the capsid protein of MPyV and super folder green fluorescent protein (sfGFP). This provides proof-of-principle that it is possible to assemble a virus like particle that is homogeneously labeled with exactly one fluorophore per structural protein. Overall, the studies detailed in this text provide insight into the mechanical properties of viruses, as well as how those properties might be utilized for biomedical applications.
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