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NMR Metabolomic Analysis of Whole Bl...

Bailey, Stasia Ann Bembenek.

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NMR Metabolomic Analysis of Whole Blood and Tissues from Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	NMR Metabolomic Analysis of Whole Blood and Tissues from Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit./
作者:	Bailey, Stasia Ann Bembenek.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	80 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-10, Section: B.
Contained By:	Dissertations Abstracts International81-10B.
標題:	Fisheries. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27785591
ISBN:	9781392549599

NMR Metabolomic Analysis of Whole Blood and Tissues from Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit.
Bailey, Stasia Ann Bembenek.

NMR Metabolomic Analysis of Whole Blood and Tissues from Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 80 p.

Source: Dissertations Abstracts International, Volume: 81-10, Section: B.

Thesis (Ph.D.)--North Carolina State University, 2019.

This item must not be sold to any third party vendors.

Sea turtles are charismatic and ecologically important animals whose populations are threatened by multiple anthropogenic factors, including chemical pollution. In heavily used shipping lanes, near oil platforms, and in the proximity of major harbors, crude oil is a pollutant that can affect sea turtles across all life stages. This research used nuclear magnetic resonance (NMR) spectroscopy to assess impacts of crude oil and Corexit 5900A, a mixture used in oil spill mitigation, on hatchling sea turtle physiology. Whole blood and tissue samples of hatchling loggerhead sea turtles (Caretta caretta) were collected after a 4-day exposure to crude oil, Corexit 5900A, a mixture of crude oil and Corexit 5900A, or a seawater control. Whole blood was processed for NMR using Drabkin's Reagent. Skeletal muscle, heart, and liver were processed with a modified Folch extraction consisting of chloroform, methanol, and water. One dimensional 1H NMR spectra were generated for each sample extract, and 2D 1H-1H and 1H-13C spectra were generated from a single or pooled sample of blood and tissue. We identified 17 metabolites in whole blood extracts, 38 metabolites in the aqueous extracts of the liver, and 30 metabolites in both the skeletal and heart muscle aqueous extracts, including organic acids/osmolytes, energy compounds, amino acids, ketone bodies, nucleosides, and nucleotides. Skeletal muscle lactate, creatines, and taurine concentrations were significantly lower in hatchlings exposed to crude oil than in control hatchlings. There was variation between hatchlings that may reflect individual response to crude oil and/or Corexit exposure. This work represents a foundation for future toxicological and physiological studies of loggerhead sea turtles and other threatened and endangered species.

ISBN: 9781392549599Subjects--Topical Terms:

609669
Fisheries.
Subjects--Index Terms:

Hatchling loggerhead sea turtles

NMR Metabolomic Analysis of Whole Blood and Tissues from Hatchling Loggerhead Sea Turtles (Caretta caretta) Experimentally Exposed to Crude Oil and/or Corexit.
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520 $a Sea turtles are charismatic and ecologically important animals whose populations are threatened by multiple anthropogenic factors, including chemical pollution. In heavily used shipping lanes, near oil platforms, and in the proximity of major harbors, crude oil is a pollutant that can affect sea turtles across all life stages. This research used nuclear magnetic resonance (NMR) spectroscopy to assess impacts of crude oil and Corexit 5900A, a mixture used in oil spill mitigation, on hatchling sea turtle physiology. Whole blood and tissue samples of hatchling loggerhead sea turtles (Caretta caretta) were collected after a 4-day exposure to crude oil, Corexit 5900A, a mixture of crude oil and Corexit 5900A, or a seawater control. Whole blood was processed for NMR using Drabkin's Reagent. Skeletal muscle, heart, and liver were processed with a modified Folch extraction consisting of chloroform, methanol, and water. One dimensional 1H NMR spectra were generated for each sample extract, and 2D 1H-1H and 1H-13C spectra were generated from a single or pooled sample of blood and tissue. We identified 17 metabolites in whole blood extracts, 38 metabolites in the aqueous extracts of the liver, and 30 metabolites in both the skeletal and heart muscle aqueous extracts, including organic acids/osmolytes, energy compounds, amino acids, ketone bodies, nucleosides, and nucleotides. Skeletal muscle lactate, creatines, and taurine concentrations were significantly lower in hatchlings exposed to crude oil than in control hatchlings. There was variation between hatchlings that may reflect individual response to crude oil and/or Corexit exposure. This work represents a foundation for future toxicological and physiological studies of loggerhead sea turtles and other threatened and endangered species.
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