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The germline-specific factor OEF-1 f...

McManus, Catherine Evans.

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The germline-specific factor OEF-1 facilitates coordinated progression through germ cell development.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The germline-specific factor OEF-1 facilitates coordinated progression through germ cell development./
作者:	McManus, Catherine Evans.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
面頁冊數:	154 p.
附註:	Source: Dissertations Abstracts International, Volume: 80-02, Section: B.
Contained By:	Dissertations Abstracts International80-02B.
標題:	Molecular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10927844
ISBN:	9780438194144

The germline-specific factor OEF-1 facilitates coordinated progression through germ cell development.
McManus, Catherine Evans.

The germline-specific factor OEF-1 facilitates coordinated progression through germ cell development. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 154 p.

Source: Dissertations Abstracts International, Volume: 80-02, Section: B.

Thesis (Ph.D.)--Yale University, 2018.

This item must not be added to any third party search indexes.

As the only cells able to transmit genetic material from generation to generation, the germ line of an organism is critical to the survival of the entire species. Germ cells are totipotent and undergo specialized processes such as meiosis and gametogenesis, meaning that somatic differentiation must be repressed in germline precursors in order to protect their unique fate. To develop into mature gametes, germ cells must transition into meiosis, initiate gamete differentiation, and pass through cell cycle checkpoints. How germ cells coordinate developmental events while protecting their unique fate is not well understood. This dissertation describes the characterization of a germ cell-specific nuclear protein OEF-1 that is important for smooth transitioning between phases of germ cell development in the nematode Caenorhabditis elegans. OEF-1 is expressed early in embryonic development in the nucleus of the germline precursor cell exclusively. Furthermore, OEF-1 expression within the adult gonad depends on gamete fate, with distinct expression patterns for the spermatogenesis program versus the oogenesis program. In oef-1 mutants, the rate of germ cell progression is accelerated, resulting in subtle defects at multiple stages of germ cell development. OEF-1 is primarily associated with the bodies of germline-expressed genes on autosomes and is excluded from the X chromosome, which is markedly depleted for germline-expressed genes. Molecularly, loss of OEF-1 intensifies the enrichment of a repressive histone modification on the X chromosome of germ cells, disrupting the balance of X to autosomal gene expression. Lastly, OEF-1 displays synthetic embryonic lethality with the methyltransferase MES-4, which plays an essential role in establishing the asymmetric chromatin status between the X and autosomes. With early, highly regulated, and tissue-specific expression, and with a potential role in chromatin regulation, this characterization of the expression, regulation, and function of OEF-1 helps to illuminate how germ cell maturation events are coordinated and coupled to chromatin regulation.

ISBN: 9780438194144Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

C.Elegans

The germline-specific factor OEF-1 facilitates coordinated progression through germ cell development.
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