東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Determining the Source of Fecal Poll...

Scopp, Anna Marie.

FindBook

Google Book

Amazon

博客來

Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking./
作者:	Scopp, Anna Marie.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
面頁冊數:	58 p.
附註:	Source: Masters Abstracts International, Volume: 80-02.
Contained By:	Masters Abstracts International80-02.
標題:	Biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10808766
ISBN:	9780438207295

Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking.
Scopp, Anna Marie.

Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 58 p.

Source: Masters Abstracts International, Volume: 80-02.

Thesis (M.S.)--University of Colorado at Denver, 2018.

This item must not be sold to any third party vendors.

Freshwater ecosystems are routinely monitored for fecal contamination to protect human health and preserve natural biodiversity. Traditionally, cultivation of fecal indicator bacteria (FIB) such as Escherichia coli is used to signify fecal contamination in a water body; however, this approach provides no information about the source of fecal contamination. In this study, Microbial Source Tracking (MST) was used to identify potential sources of fecal pollution affecting Bear Creek and Cherry Creek (Denver, CO, USA) using high-throughput 16S rRNA gene sequencing and quantitative Polymerase Chain Reaction (qPCR) of human-associated Bacteroides 16S rRNA genes. This new, multifaceted approach utilizing both qPCR and gene sequencing suggested that human fecal pollution contributes to the water column microbial communities in Bear Creek and Cherry Creek. In Bear Creek, bacteria associated with human fecal matter were distributed evenly along the creek, suggesting that there are multiple locations where human fecal contamination is entering the creek. Human fecal bacteria increased at downstream sites in Cherry Creek, suggesting that the predominant sources of human fecal pollution occur downstream of the CHERRY-07 site. Levels of potential human fecal pollution were very low in the sediments of both creeks. Sequence analyses suggested that fecal matter from Canadian geese made a very small contribution to the overall microbial community structure in water column and sediment samples (0.4% contribution on average and across nearly half of the samples). Fecal matter from other animals (e.g., duck, dog) were not identified as significant contributing sources to the Bear Creek and Cherry Creek microbial communities. Overall, this study highlights the strengths and limitations of utilizing a multifaceted MST approach (sequencing and qPCR) in ambient waters, which few other studies have demonstrated to date. Future efforts aimed at improving our understanding of fecal pollution in Denver waterways should include seasonal sampling, direct correlations between E. coli culture counts and human-associated Bacteroides qPCR counts, and testing how long human-associated Bacteroides persist in these freshwater streams after fecal contamination.

ISBN: 9780438207295Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

High-throughput gene sequencing

Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking.
LDR:03584nmm a2200409 4500 001 2272213
005 20201105105959.5
008 220629s2018 ||||||||||||||||| ||eng d
020 $a 9780438207295
035 $a (MiAaPQ)AAI10808766
035 $a (MiAaPQ)ucdenver:11030
035 $a AAI10808766
040 $a MiAaPQ $c MiAaPQ
100 1 $a Scopp, Anna Marie. $3 3549643
245 1 0 $a Determining the Source of Fecal Pollution in Urban Streams through Microbial Source Tracking.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 58 p.
500 $a Source: Masters Abstracts International, Volume: 80-02.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Mosier, Annika C.
502 $a Thesis (M.S.)--University of Colorado at Denver, 2018.
506 $a This item must not be sold to any third party vendors.
520 $a Freshwater ecosystems are routinely monitored for fecal contamination to protect human health and preserve natural biodiversity. Traditionally, cultivation of fecal indicator bacteria (FIB) such as Escherichia coli is used to signify fecal contamination in a water body; however, this approach provides no information about the source of fecal contamination. In this study, Microbial Source Tracking (MST) was used to identify potential sources of fecal pollution affecting Bear Creek and Cherry Creek (Denver, CO, USA) using high-throughput 16S rRNA gene sequencing and quantitative Polymerase Chain Reaction (qPCR) of human-associated Bacteroides 16S rRNA genes. This new, multifaceted approach utilizing both qPCR and gene sequencing suggested that human fecal pollution contributes to the water column microbial communities in Bear Creek and Cherry Creek. In Bear Creek, bacteria associated with human fecal matter were distributed evenly along the creek, suggesting that there are multiple locations where human fecal contamination is entering the creek. Human fecal bacteria increased at downstream sites in Cherry Creek, suggesting that the predominant sources of human fecal pollution occur downstream of the CHERRY-07 site. Levels of potential human fecal pollution were very low in the sediments of both creeks. Sequence analyses suggested that fecal matter from Canadian geese made a very small contribution to the overall microbial community structure in water column and sediment samples (0.4% contribution on average and across nearly half of the samples). Fecal matter from other animals (e.g., duck, dog) were not identified as significant contributing sources to the Bear Creek and Cherry Creek microbial communities. Overall, this study highlights the strengths and limitations of utilizing a multifaceted MST approach (sequencing and qPCR) in ambient waters, which few other studies have demonstrated to date. Future efforts aimed at improving our understanding of fecal pollution in Denver waterways should include seasonal sampling, direct correlations between E. coli culture counts and human-associated Bacteroides qPCR counts, and testing how long human-associated Bacteroides persist in these freshwater streams after fecal contamination.
590 $a School code: 0765.
650 4 $a Biology. $3 522710
650 4 $a Microbiology. $3 536250
650 4 $a Environmental management. $3 535182
653 $a High-throughput gene sequencing
653 $a Human fecal pollution
653 $a Microbial source tracking
653 $a Public health risk
653 $a QPCR
653 $a Recreational water quality
690 $a 0306
690 $a 0410
690 $a 0474
710 2 $a University of Colorado at Denver. $b Biology. $3 3549644
773 0 $t Masters Abstracts International $g 80-02.
790 $a 0765
791 $a M.S.
792 $a 2018
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10808766