東華大學圖書館 |

Evaluation of Strategies for Sustainable Production of Black Cohosh.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Evaluation of Strategies for Sustainable Production of Black Cohosh./
作者:	Robinson, Julia.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
面頁冊數:	122 p.
附註:	Source: Masters Abstracts International, Volume: 80-03.
Contained By:	Masters Abstracts International80-03.
標題:	Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10840751
ISBN:	9780438338296

Evaluation of Strategies for Sustainable Production of Black Cohosh.
Robinson, Julia.

Evaluation of Strategies for Sustainable Production of Black Cohosh. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 122 p.

Source: Masters Abstracts International, Volume: 80-03.

Thesis (M.S.)--North Carolina Agricultural and Technical State University, 2018.

This item must not be sold to any third party vendors.

With a reemergence of medicinal plants as medical alternatives, this research looked to help farmers develop sustainable growing strategies for Black Cohosh, a medicinal plant commonly used to treat medical issues, such as menopause. The ultimate goal was to help increase crop profitability and productivity, and decrease the habitat destruction caused by wild harvesters as well as the chance of adulterated Black Cohosh in medicine. The specific objectives were to: (1.) Determine the effect of amount of time in darkness on in vitro callus induction and shoot multiplication; (2.) Evaluate the micro- and macro- nutrient uptake of black cohosh and its impact on plant growth; and (3.) Investigate influences of shade conditions on growth and yield of plants in a high tunnel setting. Data were collected and analyzed for each growing strategy to determine the optimum growth strategy that could be implemented. Initiation of culture from leaf explants under different darkness treatments were analyzed at two different stages of tissue development. Stage 1 measured the callus development as well as shoot growth that occurred in explants, while Stage 2 compared the effect of two plant growth regulators Kinetin and Benzyladenine, on shoot growth in Black Cohosh. Leaf explants were graded at Stage 1 based on physiological characteristics of leaf explants, including color, callus development, and presence of shoot growth. Stage 2 measured the number of shoots produced per explant within culture containers between plant growth regulators and number of weeks in darkness. Overall trends indicated that increasing the length of time under darkness decreased the rate of death that occurred in leaf explants, but that the healthiest callus growth occurred when leaf explants were cultured under five weeks of darkness. Nutrient uptake was analyzed on test black cohosh plants grown under 80% shade in a greenhouse and exposed to two nutrient application treatments: Single Exposure and Continuous Exposure at 0%, 2%, 4%, 6%, 8% and 10% Fusion 360 Gold SunBurst Company Fertilizer. Growth data were collected when plants reached full growth. Nutrient uptake analysis occurred at different stages of the plant life cycle on the total amount of elements (Ca, Fe, K, Mg, Mn and Zn) after being exposed to a single dose of fertilizer solution. Continuous exposure lead to a significant decrease in stem height and diameter, and number of leaves, fresh and dry weight of rhizome and canopy size. A significant increase in number of stems was observed as fertilizer concentration increased. No noticeable plant growth change was observed with single exposure of fertilizer solution, except for a decrease in stem length when fertilizer concentration increased. In a high tunnel Black Cohosh was exposed to four treatments, 0%, 40%, 60% and 80% shading. Fully mature plants were measured and analyzed using Statistical Analysis Software University Edition, measuring the average and significant difference at the 0.05 level of significance. Canopy width (mm), canopy length (mm), total number of stems, stem diameter (mm), and stem length (mm) were all measured. Ribulose-1,5 -bisphosphate carboxylase-oxygenase was also measured in leaf samples from different shading percentages, to measure photosynthetic activity, and determine if the amount of light Black Cohosh was exposed to, would have a decreased or increased reaction on the volume of RuBisCO produced in leaves. Canopy width and canopy length measurements determined that canopy was significantly larger at the 40% level of shading, than the other shading levels. There was no significant difference in canopy from 60% - 80% shading level. Likewise, stem diameter and stem length were highest in the 40% level of shading. They were lowest at the 0% level of shading. Stem length demonstrated no significant change from 60-80% shading level, while stem length was significantly lower at 0% level then the other shading level. In reverse, total number of stems per plant is highest at the 0% shading level and lowest at the 80% level, with no significant difference between 40-60% and 40-80% shading. There was no significant change in shading levels, except between 0-40% shading for the dry weight of rhizomes. RuBisCO protein bands were determined to occur in 0%, 40%, and 60% shade levels (80% shade was not measured) with molecular weights of 55 kDa and 14 kDa, but no trends were observed based on shade level due to inferior plant samples. Protein samples were additionally measured at 60% shade level, because of longevity of 60% shade plants, but no average of RuBisCO volume could be determined due to technical error.

ISBN: 9780438338296Subjects--Topical Terms:

555447
Horticulture.
Subjects--Index Terms:

Actaea racemosa

Evaluation of Strategies for Sustainable Production of Black Cohosh.
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500 $a Source: Masters Abstracts International, Volume: 80-03.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Yang, Guochen.
502 $a Thesis (M.S.)--North Carolina Agricultural and Technical State University, 2018.
506 $a This item must not be sold to any third party vendors.
520 $a With a reemergence of medicinal plants as medical alternatives, this research looked to help farmers develop sustainable growing strategies for Black Cohosh, a medicinal plant commonly used to treat medical issues, such as menopause. The ultimate goal was to help increase crop profitability and productivity, and decrease the habitat destruction caused by wild harvesters as well as the chance of adulterated Black Cohosh in medicine. The specific objectives were to: (1.) Determine the effect of amount of time in darkness on in vitro callus induction and shoot multiplication; (2.) Evaluate the micro- and macro- nutrient uptake of black cohosh and its impact on plant growth; and (3.) Investigate influences of shade conditions on growth and yield of plants in a high tunnel setting. Data were collected and analyzed for each growing strategy to determine the optimum growth strategy that could be implemented. Initiation of culture from leaf explants under different darkness treatments were analyzed at two different stages of tissue development. Stage 1 measured the callus development as well as shoot growth that occurred in explants, while Stage 2 compared the effect of two plant growth regulators Kinetin and Benzyladenine, on shoot growth in Black Cohosh. Leaf explants were graded at Stage 1 based on physiological characteristics of leaf explants, including color, callus development, and presence of shoot growth. Stage 2 measured the number of shoots produced per explant within culture containers between plant growth regulators and number of weeks in darkness. Overall trends indicated that increasing the length of time under darkness decreased the rate of death that occurred in leaf explants, but that the healthiest callus growth occurred when leaf explants were cultured under five weeks of darkness. Nutrient uptake was analyzed on test black cohosh plants grown under 80% shade in a greenhouse and exposed to two nutrient application treatments: Single Exposure and Continuous Exposure at 0%, 2%, 4%, 6%, 8% and 10% Fusion 360 Gold SunBurst Company Fertilizer. Growth data were collected when plants reached full growth. Nutrient uptake analysis occurred at different stages of the plant life cycle on the total amount of elements (Ca, Fe, K, Mg, Mn and Zn) after being exposed to a single dose of fertilizer solution. Continuous exposure lead to a significant decrease in stem height and diameter, and number of leaves, fresh and dry weight of rhizome and canopy size. A significant increase in number of stems was observed as fertilizer concentration increased. No noticeable plant growth change was observed with single exposure of fertilizer solution, except for a decrease in stem length when fertilizer concentration increased. In a high tunnel Black Cohosh was exposed to four treatments, 0%, 40%, 60% and 80% shading. Fully mature plants were measured and analyzed using Statistical Analysis Software University Edition, measuring the average and significant difference at the 0.05 level of significance. Canopy width (mm), canopy length (mm), total number of stems, stem diameter (mm), and stem length (mm) were all measured. Ribulose-1,5 -bisphosphate carboxylase-oxygenase was also measured in leaf samples from different shading percentages, to measure photosynthetic activity, and determine if the amount of light Black Cohosh was exposed to, would have a decreased or increased reaction on the volume of RuBisCO produced in leaves. Canopy width and canopy length measurements determined that canopy was significantly larger at the 40% level of shading, than the other shading levels. There was no significant difference in canopy from 60% - 80% shading level. Likewise, stem diameter and stem length were highest in the 40% level of shading. They were lowest at the 0% level of shading. Stem length demonstrated no significant change from 60-80% shading level, while stem length was significantly lower at 0% level then the other shading level. In reverse, total number of stems per plant is highest at the 0% shading level and lowest at the 80% level, with no significant difference between 40-60% and 40-80% shading. There was no significant change in shading levels, except between 0-40% shading for the dry weight of rhizomes. RuBisCO protein bands were determined to occur in 0%, 40%, and 60% shade levels (80% shade was not measured) with molecular weights of 55 kDa and 14 kDa, but no trends were observed based on shade level due to inferior plant samples. Protein samples were additionally measured at 60% shade level, because of longevity of 60% shade plants, but no average of RuBisCO volume could be determined due to technical error.
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653 $a Rubisco
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