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Identifying Nutraceutical Supplement...

Bishop, Erin J.

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Identifying Nutraceutical Supplements that Suppress Exercise-Induced Muscle Damage Using a Novel Immune/Muscle Cell Co-culture System.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Identifying Nutraceutical Supplements that Suppress Exercise-Induced Muscle Damage Using a Novel Immune/Muscle Cell Co-culture System./
作者:	Bishop, Erin J.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:	107 p.
附註:	Source: Masters Abstracts International, Volume: 81-12.
Contained By:	Masters Abstracts International81-12.
標題:	Biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27959788
ISBN:	9798635272978

Identifying Nutraceutical Supplements that Suppress Exercise-Induced Muscle Damage Using a Novel Immune/Muscle Cell Co-culture System.
Bishop, Erin J.

Identifying Nutraceutical Supplements that Suppress Exercise-Induced Muscle Damage Using a Novel Immune/Muscle Cell Co-culture System. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 107 p.

Source: Masters Abstracts International, Volume: 81-12.

Thesis (M.S.)--University of Massachusetts Lowell, 2020.

This item must not be sold to any third party vendors.

Members of our active-duty military often endure intensive physical exercise during combat missions or even training exercises that often lead to exercise-induced muscle damage (EIMD), increasing risk of injury and diminished combat readiness. NSAIDs (nonsteroidal anti-inflammatory drugs) are commonly used to treat pain and suppress inflammation, but these drugs have multiple side effects including organ damage, thus there is great need for an alternative method of suppressing the pain, inflammation, and fatigue caused by inflammation in over-stressed muscle tissue. Currently, there is no widely utilized in vitro model that is needed to test such alternative methods, which combines muscle cells the innate immune cells that mediate inflammation, called neutrophils and macrophages. Our goal has been to create a co-culture system that accurately recapitulates in an in vitro environment the in vivo molecular interactions between injured muscles and these immune cells. Our research first identified optimal conditions for the co-culture of mouse muscle cells as they differentiate into mature muscle fibers together with differentiating neutrophils or macrophages. Next shown were the normal functional characteristics of the immune cells while being cultured in this optimal co-culture condition, including release of reactive oxygen species (ROS), one of the primary causes of inflammation during EIMD. Importantly, antioxidants are commonly advertised to combat these free radicals; however, little is known about their impact beyond neutralizing the oxidizing agents. Antioxidants can be provided by an easily digested nutritional supplement, such as fruit extracts. Therefore, studies were performed to test the capacity of nutrients to suppress ROS produced by the immune cells. Results presented indicate that antioxidant contained in the tested nutrients sequester ROS produced by both immune cell types, indicating this can be used as a nutritional intervention to suppress oxidative damage associated with EIMD. The results support our overall goal of identifying dietary supplements that will reduce muscle inflammation and fatigue commonly experienced by the Warfighter.

ISBN: 9798635272978Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

Antioxidant

Identifying Nutraceutical Supplements that Suppress Exercise-Induced Muscle Damage Using a Novel Immune/Muscle Cell Co-culture System.
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