東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

A Molecular Approach to Identify Det...

Tan, Liming.

FindBook

Google Book

Amazon

博客來

A Molecular Approach to Identify Determinants of Synaptic Specificity.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A Molecular Approach to Identify Determinants of Synaptic Specificity./
作者:	Tan, Liming.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
面頁冊數:	109 p.
附註:	Source: Dissertations Abstracts International, Volume: 78-01, Section: B.
Contained By:	Dissertations Abstracts International78-01B.
標題:	Neurosciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10112101
ISBN:	9781339752747

A Molecular Approach to Identify Determinants of Synaptic Specificity.
Tan, Liming.

A Molecular Approach to Identify Determinants of Synaptic Specificity. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 109 p.

Source: Dissertations Abstracts International, Volume: 78-01, Section: B.

Thesis (Ph.D.)--University of California, Los Angeles, 2016.

This item is not available from ProQuest Dissertations & Theses.

Information processing in the nervous system relies on precise patterns of synaptic connections between neurons. The level of synaptic specificity within the nervous system is remarkable given its tremendous complexity. How neurons can distinguish their correct synaptic partners from many other neurons during circuit assembly remains a central question in neuroscience. Although important progress has been made on molecular mechanisms regulating neural circuit assembly, the cellular recognition mechanisms mediating synaptic specificity are still poorly understood. The Drosophila visual system is well suited to uncovering the molecular mechanisms underlying synaptic specificity, because of the availability of diverse genetic tools, cell type specific markers and electron microscopic reconstruction data. In the medulla neuropil of the Drosophila visual system, different neurons form synaptic connections in different layers. Within a layer, neurons form synapses with a unique set of multiple neuronal types, which represents only a subset of neurons with processes in that layer. In my thesis research, I sought to identify candidate cell recognition molecules underlying this specificity. I did RNA sequencing on closely related neurons with different layer-specific synaptic specificities, lamina neurons L1-L5 and photoreceptor R7 and R8, at the onset of synapse formation. I showed that each of the seven cell types expresses a unique set of hundreds of genes encoding cell surface and secreted proteins. Using these data and additional localization studies on proteins tagged through modification of the endogenous locus, I demonstrated that 21 paralogs of the Dpr family, a subclass of Immunoglobulin (Ig) domain containing cell surface proteins, are expressed in unique combinations in each of the seven cell types during synapse formation. Dpr interacting proteins (DIPs), comprising nine paralogs of another subclass of Ig-containing proteins, are expressed in a complementary layer-specific fashion in subsets of synaptic partners for each of the seven cell types. Thus, I demonstrated that interacting Dprs and DIPs are expressed in synaptic partners during synapse formation in the Drosophila visual system. Furthermore, I generated null mutants of Dprs and DIPs via CRISPR-based methods, and showed that mutants of two DIPs and their cognate Dprs had similar phenotypes: neurons normally expressing these DIPs had reduced number of cells in corresponding DIP and cognate Dpr mutants. These data suggested that cognate Dprs and DIPs play important roles in the development of synaptic partners expressing them.

ISBN: 9781339752747Subjects--Topical Terms:

588700
Neurosciences.
Subjects--Index Terms:

Defective proboscis extension response

A Molecular Approach to Identify Determinants of Synaptic Specificity.
LDR:04142nmm a2200433 4500 001 2268066
005 20200810100533.5
008 220629s2016 ||||||||||||||||| ||eng d
020 $a 9781339752747
035 $a (MiAaPQ)AAI10112101
035 $a (MiAaPQ)ucla:14431
035 $a AAI10112101
040 $a MiAaPQ $c MiAaPQ
100 1 $a Tan, Liming. $3 3545321
245 1 0 $a A Molecular Approach to Identify Determinants of Synaptic Specificity.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2016
300 $a 109 p.
500 $a Source: Dissertations Abstracts International, Volume: 78-01, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Zipursky, Stephen Lawrence.
502 $a Thesis (Ph.D.)--University of California, Los Angeles, 2016.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be added to any third party search indexes.
506 $a This item must not be sold to any third party vendors.
520 $a Information processing in the nervous system relies on precise patterns of synaptic connections between neurons. The level of synaptic specificity within the nervous system is remarkable given its tremendous complexity. How neurons can distinguish their correct synaptic partners from many other neurons during circuit assembly remains a central question in neuroscience. Although important progress has been made on molecular mechanisms regulating neural circuit assembly, the cellular recognition mechanisms mediating synaptic specificity are still poorly understood. The Drosophila visual system is well suited to uncovering the molecular mechanisms underlying synaptic specificity, because of the availability of diverse genetic tools, cell type specific markers and electron microscopic reconstruction data. In the medulla neuropil of the Drosophila visual system, different neurons form synaptic connections in different layers. Within a layer, neurons form synapses with a unique set of multiple neuronal types, which represents only a subset of neurons with processes in that layer. In my thesis research, I sought to identify candidate cell recognition molecules underlying this specificity. I did RNA sequencing on closely related neurons with different layer-specific synaptic specificities, lamina neurons L1-L5 and photoreceptor R7 and R8, at the onset of synapse formation. I showed that each of the seven cell types expresses a unique set of hundreds of genes encoding cell surface and secreted proteins. Using these data and additional localization studies on proteins tagged through modification of the endogenous locus, I demonstrated that 21 paralogs of the Dpr family, a subclass of Immunoglobulin (Ig) domain containing cell surface proteins, are expressed in unique combinations in each of the seven cell types during synapse formation. Dpr interacting proteins (DIPs), comprising nine paralogs of another subclass of Ig-containing proteins, are expressed in a complementary layer-specific fashion in subsets of synaptic partners for each of the seven cell types. Thus, I demonstrated that interacting Dprs and DIPs are expressed in synaptic partners during synapse formation in the Drosophila visual system. Furthermore, I generated null mutants of Dprs and DIPs via CRISPR-based methods, and showed that mutants of two DIPs and their cognate Dprs had similar phenotypes: neurons normally expressing these DIPs had reduced number of cells in corresponding DIP and cognate Dpr mutants. These data suggested that cognate Dprs and DIPs play important roles in the development of synaptic partners expressing them.
590 $a School code: 0031.
650 4 $a Neurosciences. $3 588700
650 4 $a Genetics. $3 530508
650 4 $a Developmental biology. $3 592588
653 $a Defective proboscis extension response
653 $a DPR interacting proteins
653 $a Drosophila
653 $a Immunoglobulin superfamily proteins
653 $a Synaptic specificity
653 $a Visual system
690 $a 0317
690 $a 0369
690 $a 0758
710 2 $a University of California, Los Angeles. $b Biological Chemistry. $3 3545322
773 0 $t Dissertations Abstracts International $g 78-01B.
790 $a 0031
791 $a Ph.D.
792 $a 2016
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10112101