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Transcriptional Regulator Coding-Seq...

Britton, Candace.

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Transcriptional Regulator Coding-Sequence Evolution Preceded cis-Regulatory Changes in the Origin of a New Transcriptional Circuit.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Transcriptional Regulator Coding-Sequence Evolution Preceded cis-Regulatory Changes in the Origin of a New Transcriptional Circuit./
作者:	Britton, Candace.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	127 p.
附註:	Source: Dissertations Abstracts International, Volume: 80-10, Section: B.
Contained By:	Dissertations Abstracts International80-10B.
標題:	Molecular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13807533
ISBN:	9781392048436

Transcriptional Regulator Coding-Sequence Evolution Preceded cis-Regulatory Changes in the Origin of a New Transcriptional Circuit.
Britton, Candace.

Transcriptional Regulator Coding-Sequence Evolution Preceded cis-Regulatory Changes in the Origin of a New Transcriptional Circuit. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 127 p.

Source: Dissertations Abstracts International, Volume: 80-10, Section: B.

Thesis (Ph.D.)--University of California, San Francisco, 2019.

This item must not be sold to any third party vendors.

Life often diversifies through changes in gene expression patterns. These patterns evolve via changes in transcriptional regulatory circuits that are determined by transcriptional regulatory proteins and the cis-regulatory sequences they bind in the genome. While it has long been known that changes in cis-regulatory sequences can affect the evolution of gene expression patterns and that transcriptional regulatory proteins can themselves evolve, we know little of how these two types of regulatory changes occur together to generate new circuits. I discerned a stepwise order of evolutionary events in which both regulator protein-coding and cis-regulatory changes were necessary to evolve a new transcriptional regulatory circuit (repression of the a-specific genes by Matα2 in yeast). The two changes evolved at separate points in time, millions of years apart. First to evolve were coding-sequence changes in the regulator that formed new protein-protein interaction regions. In one lineage, these new protein-protein interactions became necessary for Matα2's ancestral gene regulatory function (repression of the haploid-specific genes with Mata1). In another lineage, millions of years after the coding-sequence changes to Matα2, cis-regulatory changes occurred in the a-specific genes, thereby co-opting Matα2 for regulation of this new set of target genes. We propose that this evolutionary trajectory is an example of constructive neutral evolution in that Matα2's new protein-protein interactions initially had no consequence to the logic of cell-type specific gene regulation, but eventually allowed for the creation of a novel circuit (Chapter 2). In the course of these investigations, I also observed additional coding-sequence changes in the DNA-binding domain of Matα2 (Chapter 3), and evolutionary changes in the identities of some of the yeast cell-type specific genes (Chapter 4). The results presented here add to our understanding of the ways in which transcriptional regulatory circuits diversify.

ISBN: 9781392048436Subjects--Topical Terms:

517296
Molecular biology.

Transcriptional Regulator Coding-Sequence Evolution Preceded cis-Regulatory Changes in the Origin of a New Transcriptional Circuit.
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