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Polyamines Regulate Chilling Respons...

Wan Hussin, Wan Mohd Reza Ikwan Bin.

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Polyamines Regulate Chilling Response in Ripening Tomato Fruits by De Novo Transcription.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Polyamines Regulate Chilling Response in Ripening Tomato Fruits by De Novo Transcription./
Author:	Wan Hussin, Wan Mohd Reza Ikwan Bin.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
Description:	100 p.
Notes:	Source: Masters Abstracts International, Volume: 79-09.
Contained By:	Masters Abstracts International79-09.
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10685189
ISBN:	9780355612974

Polyamines Regulate Chilling Response in Ripening Tomato Fruits by De Novo Transcription.
Wan Hussin, Wan Mohd Reza Ikwan Bin.

Polyamines Regulate Chilling Response in Ripening Tomato Fruits by De Novo Transcription. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 100 p.

Source: Masters Abstracts International, Volume: 79-09.

Thesis (M.S.)--Purdue University, 2017.

This item must not be sold to any third party vendors.

Plants response to various abiotic stress, including chilling injury, by differentially regulating genes responsible for protection against these stresses. Polyamines (PAs); putrescine (Put), spermidine (Spd), and spermine (Spm), are ubiquitous metabolite in all organisms and have been implicated in abiotic stresses in plants. Dr. Handa laboratory has previously developed high Spd/Spm tomato fruits genotypes by expressing yeast S-adenosylmethionine decarboxylase (SAMDC). In present investigation, the role of Spd/Spm in chilling injury was evaluated by using two independent transgenic lines with high Spd/Spm (556 and 579) and isogenic parental Ohio 8245 (WT) genotypes. Fruits at onset of ripening (Breaker, BR) and fully ripe (Breaker+8 days, BR+8) from both transgenic lines and WT were subjected to chilling by storing at 5°C or 8°C. Results showed that the 556 and 579 fruits exhibit more chilling tolerance phenotype at BR+8 stage but performed poorly at BR stage compared to WT fruit. RNA-seq analyses of transcriptome showed differential expression of a large number of genes in response to chilling treatment of high Spd/Spm fruit compared to WT fruit, which suggesting a significant effect of PAs in chilling response mechanism. PAs are implicated in regulating genes related to redox homeostasis (Thioredoxin family protein), stress signaling pathway (MAPK cascade), various cold responsive genes, protein chaperon (heat shock proteins) and cellular antioxidant system (CAT, SOD and APX). It can be postulated that, being positive charged molecules, PAs bind to DNA, RNA and transcription factors and differentially regulate expression of genes associated with chilling tolerance in ripened fruits that help acclimate fruits to sub optimal chilling temperatures.

ISBN: 9780355612974Subjects--Topical Terms:

517296
Molecular biology.

Polyamines Regulate Chilling Response in Ripening Tomato Fruits by De Novo Transcription.
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