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Injectable Gels with Potential Use i...

Montatskiy, Maksim.

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Injectable Gels with Potential Use in Endodontics.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Injectable Gels with Potential Use in Endodontics./
Author:	Montatskiy, Maksim.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	63 p.
Notes:	Source: Masters Abstracts International, Volume: 80-10.
Contained By:	Masters Abstracts International80-10.
Subject:	Dentistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13813281
ISBN:	9781392027615

Injectable Gels with Potential Use in Endodontics.
Montatskiy, Maksim.

Injectable Gels with Potential Use in Endodontics. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 63 p.

Source: Masters Abstracts International, Volume: 80-10.

Thesis (M.S.)--Marquette University, 2019.

This item must not be added to any third party search indexes.

Objective: To formulate a thermoreversible hydrogel using methyl-cellulose, carbon dots and inorganic salts to achieve physiologically compatible gelation temperature; to test cytotoxic effects of formulation against dental pulp stem cells. Materials/Methods: A 5% w/v methylcellulose (MC) solution was prepared and equal volumes of NaCl aqueous solution were added to make various sodium chloride salt concentrations and reach the final MC concentration of 2.5%. Samples were analyzed using a shear rheometer to monitor complex shear viscosity as a function of temperature to arrive at onset gelation temperature closest to targeted temperature. Carbon dots were synthesized by thermal method using ammonium citrate as nitrogen precursor. Dental pulp stem cells were isolated from extracted third molars and cultured under standard aseptic conditions. Cells were seeded onto well plates containing optimized hydrogels and cell proliferations were monitored at 1, 2, 5 and 14 days. Cytotoxicity of the samples was tested against a positive control (UltraCal Calcium Hydroxide) and a negative control (cells with no gel). Results: The optimal gelation temperature (30-37 °C) was achieved by using 2.5% methylcellulose, 8.0% sodium chloride and 5 ug/mL carbon dots. The gelation temperature was found to be significantly affected by the salt concentration and slightly affected by concentration of carbon dots. The gelation temperature was inversely proportional to the NaCl concentration. Cytotoxicity testing of hydrogels revealed adequate proliferation of dental pulp stem cells when compared to values produced by calcium hydroxide, indicating a level of biocompatibility conducive to proliferation of these cells. Conclusion: Cellulose-based hydrogels incorporating carbon dots can be considered as a potential formulation for use as a scaffold during regenerative endodontic procedure. This study utilizes a simple, inexpensive approach to fabricate such an injectable non- cytotoxic gel. However, antibacterial effect of the gel needs to be evaluated and is the subject of our future studies.

ISBN: 9781392027615Subjects--Topical Terms:

828971
Dentistry.

Injectable Gels with Potential Use in Endodontics.
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245 1 0 $a Injectable Gels with Potential Use in Endodontics.
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300 $a 63 p.
500 $a Source: Masters Abstracts International, Volume: 80-10.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Tayebi, Lobat.
502 $a Thesis (M.S.)--Marquette University, 2019.
506 $a This item must not be added to any third party search indexes.
506 $a This item must not be sold to any third party vendors.
520 $a Objective: To formulate a thermoreversible hydrogel using methyl-cellulose, carbon dots and inorganic salts to achieve physiologically compatible gelation temperature; to test cytotoxic effects of formulation against dental pulp stem cells. Materials/Methods: A 5% w/v methylcellulose (MC) solution was prepared and equal volumes of NaCl aqueous solution were added to make various sodium chloride salt concentrations and reach the final MC concentration of 2.5%. Samples were analyzed using a shear rheometer to monitor complex shear viscosity as a function of temperature to arrive at onset gelation temperature closest to targeted temperature. Carbon dots were synthesized by thermal method using ammonium citrate as nitrogen precursor. Dental pulp stem cells were isolated from extracted third molars and cultured under standard aseptic conditions. Cells were seeded onto well plates containing optimized hydrogels and cell proliferations were monitored at 1, 2, 5 and 14 days. Cytotoxicity of the samples was tested against a positive control (UltraCal Calcium Hydroxide) and a negative control (cells with no gel). Results: The optimal gelation temperature (30-37 °C) was achieved by using 2.5% methylcellulose, 8.0% sodium chloride and 5 ug/mL carbon dots. The gelation temperature was found to be significantly affected by the salt concentration and slightly affected by concentration of carbon dots. The gelation temperature was inversely proportional to the NaCl concentration. Cytotoxicity testing of hydrogels revealed adequate proliferation of dental pulp stem cells when compared to values produced by calcium hydroxide, indicating a level of biocompatibility conducive to proliferation of these cells. Conclusion: Cellulose-based hydrogels incorporating carbon dots can be considered as a potential formulation for use as a scaffold during regenerative endodontic procedure. This study utilizes a simple, inexpensive approach to fabricate such an injectable non- cytotoxic gel. However, antibacterial effect of the gel needs to be evaluated and is the subject of our future studies.
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