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Progenitor Cell Contributions to Mai...

Weng, Pei-Lun.

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Progenitor Cell Contributions to Maintenance and Regeneration in Salivary Gland and Olfactory Epithelium.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Progenitor Cell Contributions to Maintenance and Regeneration in Salivary Gland and Olfactory Epithelium./
作者:	Weng, Pei-Lun.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
面頁冊數:	160 p.
附註:	Source: Dissertations Abstracts International, Volume: 80-04, Section: B.
Contained By:	Dissertations Abstracts International80-04B.
標題:	Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10842877
ISBN:	9780438380998

Progenitor Cell Contributions to Maintenance and Regeneration in Salivary Gland and Olfactory Epithelium.
Weng, Pei-Lun.

Progenitor Cell Contributions to Maintenance and Regeneration in Salivary Gland and Olfactory Epithelium. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 160 p.

Source: Dissertations Abstracts International, Volume: 80-04, Section: B.

Thesis (Ph.D.)--University of Rochester, 2018.

This item must not be added to any third party search indexes.

Embryonic development of the salivary gland is well characterized, but the origin of adult secretory acinar cells during normal turnover or regeneration has been unclear. There is a significant body of literature suggesting that the adult salivary gland has bipotent stem cells responsible for tissue homeostasis and regeneration after injury. Various putative stem cells have been identified, including cells marked by expression of the basic helix-loop-helix transcription factor Ascl3. Ascl genes, members of the achaete scute-like family, are expressed in progenitor cells of many tissues. Early work in this thesis more closely investigated the nature of the Ascl3+ cells. The results showed that in the olfactory epithelium, Ascl3 expression marks progenitor cells that are lineage committed to generate microvillar cells and Bowman's glands, but that Ascl3 expression does not mark the multipotent stem cells. To further examine the origin of differentiated acinar cells in the salivary gland, two independent genetic models were used to investigate putative stem cells. Excretory and intercalated ducts are proposed to be the location of adult salivary gland stem cells. Keratin 5 (K5) is expressed in basal excretory duct and intercalated duct cells, and Axin 2, a specific target of the Wnt-signaling pathway, labels intercalated duct cells. This work showed that under normal homeostasis, K5- and Axin 2-labeled duct cells do not participate in acinar cell replacement. In addition, these cells are not involved in regeneration of acinar cells after ductal ligation injury. Surprisingly, after severe irradiation-induced salivary gland damage, K5- and Axin 2-labeled intercalated duct cells do generate acinar cells. Furthermore, the duct-derived acinar cells express several functional markers for secretion. This is the first in vivo lineage tracing analysis indicating that duct cells can regenerate acinar cells. These results suggest that the cellular plasticity of the duct cells provides an alternative route to rescue salivary gland function after irradiation-induced injury. This study opens an exciting possibility for future directions in engineering the regeneration of acinar cells for those head and neck cancer patients with radiation-induced dry mouth (xerostomia).

ISBN: 9780438380998Subjects--Topical Terms:

643180
Pathology.

Progenitor Cell Contributions to Maintenance and Regeneration in Salivary Gland and Olfactory Epithelium.
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520 $a Embryonic development of the salivary gland is well characterized, but the origin of adult secretory acinar cells during normal turnover or regeneration has been unclear. There is a significant body of literature suggesting that the adult salivary gland has bipotent stem cells responsible for tissue homeostasis and regeneration after injury. Various putative stem cells have been identified, including cells marked by expression of the basic helix-loop-helix transcription factor Ascl3. Ascl genes, members of the achaete scute-like family, are expressed in progenitor cells of many tissues. Early work in this thesis more closely investigated the nature of the Ascl3+ cells. The results showed that in the olfactory epithelium, Ascl3 expression marks progenitor cells that are lineage committed to generate microvillar cells and Bowman's glands, but that Ascl3 expression does not mark the multipotent stem cells. To further examine the origin of differentiated acinar cells in the salivary gland, two independent genetic models were used to investigate putative stem cells. Excretory and intercalated ducts are proposed to be the location of adult salivary gland stem cells. Keratin 5 (K5) is expressed in basal excretory duct and intercalated duct cells, and Axin 2, a specific target of the Wnt-signaling pathway, labels intercalated duct cells. This work showed that under normal homeostasis, K5- and Axin 2-labeled duct cells do not participate in acinar cell replacement. In addition, these cells are not involved in regeneration of acinar cells after ductal ligation injury. Surprisingly, after severe irradiation-induced salivary gland damage, K5- and Axin 2-labeled intercalated duct cells do generate acinar cells. Furthermore, the duct-derived acinar cells express several functional markers for secretion. This is the first in vivo lineage tracing analysis indicating that duct cells can regenerate acinar cells. These results suggest that the cellular plasticity of the duct cells provides an alternative route to rescue salivary gland function after irradiation-induced injury. This study opens an exciting possibility for future directions in engineering the regeneration of acinar cells for those head and neck cancer patients with radiation-induced dry mouth (xerostomia).
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