語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Local Nucleic Acid Base Conformation...
~
Ji, Huiying.
FindBook
Google Book
Amazon
博客來
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA./
作者:
Ji, Huiying.
出版者:
Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:
180 p.
附註:
Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.
Contained By:
Dissertation Abstracts International80-08B(E).
標題:
Chemistry. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13425709
ISBN:
9781392059418
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
Ji, Huiying.
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
- Ann Arbor : ProQuest Dissertations & Theses, 2019 - 180 p.
Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.
Thesis (Ph.D.)--University of Oregon, 2019.
Understanding the local conformations of DNA at the level of individual nucleic acid bases is important for the study of the mechanism of DNA sequence-dependent behavior. Here we apply linear absorption, circular dichroism (CD), and fluorescence spectroscopy to study the DNA local base conformation using 6-methyl Isoxanthopterin (6-MI) labeled DNA. We interpret excitation--emission peak shift (EES) measurements of the 6-MI, both as a ribonucleotide monophosphate in solution and as a site-specific substituent for guanine in various DNA constructs, by implementing a simple two-state model. We show that the spectroscopic properties of the 6-MI probe in DNA can be used to obtain detailed information about local base conformations and conformational heterogeneity and fluctuations. Based on these findings, we apply a simple theoretical model to calculate CD of 6-MI substituted DNA constructs. We find that our model can be used to extract basesequence- dependent information about the local conformation of the 6-MI probe as modulated by the local base or base-pair environment. We next apply 6-MI to probe the ligand insertion of small molecules to duplex DNA, further extending the potential of 6- MI as a useful reporter of local nucleic acid base conformation. These studies served to establish a new level of sophistication in qualitatively analyzing 6-MI structural behavior in terms of local base stacking and unstacking conformations.
ISBN: 9781392059418Subjects--Topical Terms:
516420
Chemistry.
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
LDR
:02583nmm a2200325 4500
001
2204061
005
20190624102129.5
008
201008s2019 ||||||||||||||||| ||eng d
020
$a
9781392059418
035
$a
(MiAaPQ)AAI13425709
035
$a
(MiAaPQ)oregon:12387
035
$a
AAI13425709
040
$a
MiAaPQ
$c
MiAaPQ
100
1
$a
Ji, Huiying.
$0
(orcid)0000-0002-5036-2611
$3
3430879
245
1 0
$a
Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6-Methyl Isoxanthopterin (6-MI) Labeled DNA.
260
1
$a
Ann Arbor :
$b
ProQuest Dissertations & Theses,
$c
2019
300
$a
180 p.
500
$a
Source: Dissertation Abstracts International, Volume: 80-08(E), Section: B.
500
$a
Advisers: Andrew H. Marcud; Peter H. von Hippel.
502
$a
Thesis (Ph.D.)--University of Oregon, 2019.
520
$a
Understanding the local conformations of DNA at the level of individual nucleic acid bases is important for the study of the mechanism of DNA sequence-dependent behavior. Here we apply linear absorption, circular dichroism (CD), and fluorescence spectroscopy to study the DNA local base conformation using 6-methyl Isoxanthopterin (6-MI) labeled DNA. We interpret excitation--emission peak shift (EES) measurements of the 6-MI, both as a ribonucleotide monophosphate in solution and as a site-specific substituent for guanine in various DNA constructs, by implementing a simple two-state model. We show that the spectroscopic properties of the 6-MI probe in DNA can be used to obtain detailed information about local base conformations and conformational heterogeneity and fluctuations. Based on these findings, we apply a simple theoretical model to calculate CD of 6-MI substituted DNA constructs. We find that our model can be used to extract basesequence- dependent information about the local conformation of the 6-MI probe as modulated by the local base or base-pair environment. We next apply 6-MI to probe the ligand insertion of small molecules to duplex DNA, further extending the potential of 6- MI as a useful reporter of local nucleic acid base conformation. These studies served to establish a new level of sophistication in qualitatively analyzing 6-MI structural behavior in terms of local base stacking and unstacking conformations.
520
$a
This dissertation contains previously published and unpublished co-authored material.
590
$a
School code: 0171.
650
4
$a
Chemistry.
$3
516420
650
4
$a
Physical chemistry.
$3
1981412
650
4
$a
Molecular biology.
$3
517296
690
$a
0485
690
$a
0494
690
$a
0307
710
2
$a
University of Oregon.
$b
Department of Chemistry and Biochemistry.
$3
3181305
773
0
$t
Dissertation Abstracts International
$g
80-08B(E).
790
$a
0171
791
$a
Ph.D.
792
$a
2019
793
$a
English
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13425709
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9380610
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入