東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Salmonella enterica Stress Tolerance...

Wahlig, Taylor.

FindBook

Google Book

Amazon

博客來

Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy./
作者:	Wahlig, Taylor.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	204 p.
附註:	Source: Dissertation Abstracts International, Volume: 80-06(E), Section: B.
Contained By:	Dissertation Abstracts International80-06B(E).
標題:	Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13427529
ISBN:	9780438830684

Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy.
Wahlig, Taylor.

Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 204 p.

Source: Dissertation Abstracts International, Volume: 80-06(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2019.

Salmonella enterica is a successful pathogen with a broad host range. Phenotypic heterogeneity between the more than 5000 Salmonella serovars and heterogeneity within strains contribute to this success. Additionally, robust and redundant stress response mechanisms allow for survival in the environment and host. This thesis presents an example of redundant reactive oxygen species (ROS) neutralizing enzymes that contribute to growth on plants and examples of phenotypic heterogeneity driven by the pleiotropic effects of a single nucleotide polymorphism in the Lipopolysaccharide Transport G (lptG) gene.

ISBN: 9780438830684Subjects--Topical Terms:

536250
Microbiology.

Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy.
LDR:03577nmm a2200349 4500 001 2203778
005 20190606091703.5
008 201008s2019 ||||||||||||||||| ||eng d
020 $a 9780438830684
035 $a (MiAaPQ)AAI13427529
035 $a (MiAaPQ)wisc:15989
035 $a AAI13427529
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wahlig, Taylor. $3 3430583
245 1 0 $a Salmonella enterica Stress Tolerance: Genetic Redundancy and Pleiotropy.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 204 p.
500 $a Source: Dissertation Abstracts International, Volume: 80-06(E), Section: B.
500 $a Adviser: Charles Kaspar.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2019.
520 $a Salmonella enterica is a successful pathogen with a broad host range. Phenotypic heterogeneity between the more than 5000 Salmonella serovars and heterogeneity within strains contribute to this success. Additionally, robust and redundant stress response mechanisms allow for survival in the environment and host. This thesis presents an example of redundant reactive oxygen species (ROS) neutralizing enzymes that contribute to growth on plants and examples of phenotypic heterogeneity driven by the pleiotropic effects of a single nucleotide polymorphism in the Lipopolysaccharide Transport G (lptG) gene.
520 $a Salmonella has efficient and redundant mechanisms to neutralize ROS, which allow it to overcome ROS bursts both within macrophage and when eliciting plant immune responses. In Chapter 2, I show that growth of Salmonella enterica strain 14028S was not impacted by plant produced ROS. In contrast, growth of strain LT2, which has reduced ROS tolerance due to inactivation of rpoS, was impacted by plant produced ROS.
520 $a Broad acting stress tolerance regulons, like the RpoS regulon, significantly impact Salmonella stress tolerance. Minor genetic alterations outside of central regulators can also impact Salmonella stress tolerance. In chapters 3 and 4, I characterized a Salmonella enterica strain E40 isolate (E40V) with increased tolerance to bile salts that was driven by a single nucleotide polymorphism (SNP) in lptG. Transcriptional profiling of E40 and E40V cells grown in TSB revealed greater expression of yjbE (an extracellular polymeric substance (EPS) production gene), yciE (a putative stress response protein) and Acid Shock Protein Precursor in E40V. In addition to increased bile salts tolerance, E40V had increased survival in acid and EPS production, which corresponded with the observed transcriptional differences.
520 $a While only three genes were differentially expressed between E40 and E40V in TSB, hundreds of genes were differentially expressed when the cells were grown in TSB with bile salts. E40V had greater expression of stress response regulators and chaperones, while E40 had greater expression of fimbrial genes and Salmonella Pathogenicity Island 1 (SPI-1) genes. Compared to E40V, E40 had more pellicle formation and had greater invasion of Caco-2 cells. Salmonella utilize a variety of strategies to succeed as pathogens. Presented here is an example of redundancy in a critical function that contributes to growth outside of the host and an example of phenotypic heterogeneity that impacts population level fitness.
590 $a School code: 0262.
650 4 $a Microbiology. $3 536250
650 4 $a Genetics. $3 530508
650 4 $a Physiology. $3 518431
690 $a 0410
690 $a 0369
690 $a 0719
710 2 $a The University of Wisconsin - Madison. $b Microbiology. $3 2099954
773 0 $t Dissertation Abstracts International $g 80-06B(E).
790 $a 0262
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13427529