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Simultaneous Quantitative Detection ...

Frig, Natasha Marie.

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Simultaneous Quantitative Detection of Nonsteroidal Anti-inflammatory Drugs in Equine Plasma Using High Performance Liquid Chromatography Coupled with Strong Anion Solid Phase Extraction.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Simultaneous Quantitative Detection of Nonsteroidal Anti-inflammatory Drugs in Equine Plasma Using High Performance Liquid Chromatography Coupled with Strong Anion Solid Phase Extraction./
Author:	Frig, Natasha Marie.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	63 p.
Notes:	Source: Masters Abstracts International, Volume: 58-01.
Contained By:	Masters Abstracts International58-01(E).
Subject:	Analytical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10842736
ISBN:	9780438353756

Simultaneous Quantitative Detection of Nonsteroidal Anti-inflammatory Drugs in Equine Plasma Using High Performance Liquid Chromatography Coupled with Strong Anion Solid Phase Extraction.
Frig, Natasha Marie.

Simultaneous Quantitative Detection of Nonsteroidal Anti-inflammatory Drugs in Equine Plasma Using High Performance Liquid Chromatography Coupled with Strong Anion Solid Phase Extraction. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 63 p.

Source: Masters Abstracts International, Volume: 58-01.

Thesis (M.S.)--Western Illinois University, 2018.

Doping control has become an important regulation by the United States Equestrian Federation (USEF). Nonsteroidal anti-inflammatory drugs (NSAIDs) are therapeutically used to treat joint, musculoskeletal, and bone inflammation, laminitis, and gastrointestinal pain through the inhibition of cyclooxygenase (COX) 2 enzyme. The USEF set unique thresholds for the seven NSAIDs to limit the adverse effects caused by the inhibition of COX-1 enzymes. Concentrations above these thresholds cause ulcers, damage to repair mechanisms, and can mask the lameness of the horse. As a result, the quantification and detection of NSAIDs in equine plasma is of interest. In this research, method development optimized both a high-performance liquid chromatography (HPLC) and a strong anion solid phase extraction (SPE) method with internal standard calibration. A reverse phase C18 column with UV-Vis detection (254 nm) determined the optimized HPLC conditions as isocratic 95:5 methanol:acetonitrile (70%) and water (30%) in 0.1% acetic acid. Optimization of the strong anion SPE led to discovery that the presence of plasma residues slightly overlapped with NSAID signals and therefore 68% organic solvent was used rather than 70% to resolve the issue. Recovery of NSAIDs after strong anion SPE were as follows: oxyphenbutazone (76%), ketoprofen (97%), naproxen (99%), phenylbutazone (69%), flunixin (61%), diclofenac (94%), and meclofenamic acid (94%). Method validation parameters determined by ISO 17025 were successfully applied, i.e. precision, accuracy, and limit of quantification (LOQ). The precision (%RSD) for low, medium, and high controls were less than 17%, 8%, and 2%, respectively. Accuracy (% error) for low, medium, and high controls were less than +/-10%, +/-5%, and +/-2%, respectively. The LOQ values for oxyphenbutazone, ketoprofen, naproxen, phenylbutazone, flunixin, diclofenac, and meclofenamic acid in equine plasma (200 microL) were determined as 0.03, 0.01, 0.03, 0.01, 0.01, 0.04, and 0.06, respectively.

ISBN: 9780438353756Subjects--Topical Terms:

3168300
Analytical chemistry.

Simultaneous Quantitative Detection of Nonsteroidal Anti-inflammatory Drugs in Equine Plasma Using High Performance Liquid Chromatography Coupled with Strong Anion Solid Phase Extraction.
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520 $a Doping control has become an important regulation by the United States Equestrian Federation (USEF). Nonsteroidal anti-inflammatory drugs (NSAIDs) are therapeutically used to treat joint, musculoskeletal, and bone inflammation, laminitis, and gastrointestinal pain through the inhibition of cyclooxygenase (COX) 2 enzyme. The USEF set unique thresholds for the seven NSAIDs to limit the adverse effects caused by the inhibition of COX-1 enzymes. Concentrations above these thresholds cause ulcers, damage to repair mechanisms, and can mask the lameness of the horse. As a result, the quantification and detection of NSAIDs in equine plasma is of interest. In this research, method development optimized both a high-performance liquid chromatography (HPLC) and a strong anion solid phase extraction (SPE) method with internal standard calibration. A reverse phase C18 column with UV-Vis detection (254 nm) determined the optimized HPLC conditions as isocratic 95:5 methanol:acetonitrile (70%) and water (30%) in 0.1% acetic acid. Optimization of the strong anion SPE led to discovery that the presence of plasma residues slightly overlapped with NSAID signals and therefore 68% organic solvent was used rather than 70% to resolve the issue. Recovery of NSAIDs after strong anion SPE were as follows: oxyphenbutazone (76%), ketoprofen (97%), naproxen (99%), phenylbutazone (69%), flunixin (61%), diclofenac (94%), and meclofenamic acid (94%). Method validation parameters determined by ISO 17025 were successfully applied, i.e. precision, accuracy, and limit of quantification (LOQ). The precision (%RSD) for low, medium, and high controls were less than 17%, 8%, and 2%, respectively. Accuracy (% error) for low, medium, and high controls were less than +/-10%, +/-5%, and +/-2%, respectively. The LOQ values for oxyphenbutazone, ketoprofen, naproxen, phenylbutazone, flunixin, diclofenac, and meclofenamic acid in equine plasma (200 microL) were determined as 0.03, 0.01, 0.03, 0.01, 0.01, 0.04, and 0.06, respectively.
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